Recombinant HRP Anti-ELMO1 antibody [EPR12919] (ab204386)
![Western blot - HRP Anti-ELMO1 antibody [EPR12919] (ab204386)](https://www.abcam.com/ps/products/204/ab204386/Images/ab204386-252323-WBab2043861.jpg "Western blot - HRP Anti-ELMO1 antibody [EPR12919] (ab204386)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR12919] to ELMO1
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
Overview
-
Product name
HRP Anti-ELMO1 antibody [EPR12919]
See all ELMO1 primary antibodies -
Description
HRP Rabbit monoclonal [EPR12919] to ELMO1 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human placenta tissue and Jurkat whole cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12919 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
|
| Notes |
|---|
|
WB
1/5000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa). |
Target
-
Function
Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. -
Tissue specificity
Widely expressed, with a higher expression in the spleen and placenta. -
Sequence similarities
Contains 1 ELMO domain.
Contains 1 PH domain. -
Post-translational
modificationsPhosphorylated by HCK. -
Cellular localization
Cytoplasm. Cell membrane. Translocation to plasma membrane seems to be mediated by DOCK1 and CRK. - Information by UniProt
-
Database links
- Entrez Gene: 9844 Human
- Entrez Gene: 140580 Mouse
- Entrez Gene: 361251 Rat
- Omim: 606420 Human
- SwissProt: Q92556 Human
- SwissProt: Q8BPU7 Mouse
- Unigene: 656638 Human
- Unigene: 342392 Mouse
-
Alternative names
- CED 12 antibody
- Ced 12 homolog 1 antibody
- Ced 12 homolog antibody
see all
Images
-
Western blot - HRP Anti-ELMO1 antibody [EPR12919] (ab204386)All lanes : HRP Anti-ELMO1 antibody [EPR12919] (ab204386) at 1/5000 dilution
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab204386 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
-
![HRP Anti-ELMO1 antibody [EPR12919] (ab204386)](https://www.abcam.com/ps/products/204/ab204386/Images/ab204386-3-benefits-of-recombinant-antibodies.png)
HRP Anti-ELMO1 antibody [EPR12919] (ab204386)
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab204386 has not yet been referenced specifically in any publications.