HRP Anti-GST antibody (ab3416)
Key features and details
- HRP Rabbit polyclonal to GST
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-GST antibody
See all GST primary antibodies -
Description
HRP Rabbit polyclonal to GST -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Full length native protein (purified) corresponding to Schistosoma japonicum GST. Glutathione-S-transferase (GST) from Schistosoma japonicum.
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General notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.00
Constituents: 3% Sodium deoxycholate, 3% Triton-X-100, 0.3% Tris HCl, 15% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Immunoaffinity purified using GST immobilized on a solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Recombinant Protein
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Related Products
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/5000.
|
ELISA | (2) |
1/10000 - 1/25000.
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Notes |
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WB
1/1000 - 1/5000. |
ELISA
1/10000 - 1/25000. |
Target
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Relevance
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. -
Alternative names
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
see all
Images
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Graph of ELISA measuring levels of bound GST-tagged mosquito proteins. 5g of target protein was coated onto an ELISA plate and incubated overnight at 4°C. The plate was washed and GST-tagged mosquito proteins were incubated for an hour at RT. The proteins were washed off, GST-HRP antibody (ab3416) was diluted 1/5000 in 1%BSA in PBS and added for 30 min at RT. The plate was washed and TMB substrate was added for 20 min at RT. Stop solution was added and the O.D. of the wells read at 450 nm.
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Graph of ELISA measuring levels of bound GST-tagged mosquito proteins. 5g of target protein was coated onto an ELISA plate and incubated overnight at 4°C. The plate was washed and GST-tagged mosquito proteins were incubated for an hour at RT. The proteins were washed off, GST-HRP antibody (ab3416) was diluted 1/5000 in 1%BSA in PBS and added for 30 min at RT. The plate was washed and TMB substrate was added for 20 min at RT. Stop solution was added and the O.D. of the wells read at 450 nm.
Datasheets and documents
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SDS download
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Datasheet download
References (30)
ab3416 has been referenced in 30 publications.
- Binda O et al. SMA-linked SMN mutants prevent phase separation properties and SMN interactions with FMRP family members. Life Sci Alliance 6:N/A (2023). PubMed: 36375840
- Kim JS et al. Arabidopsis TBP-ASSOCIATED FACTOR 12 ortholog NOBIRO6 controls root elongation with unfolded protein response cofactor activity. Proc Natl Acad Sci U S A 119:N/A (2022). PubMed: 35115407
- Won SY et al. p21-activated kinase 4 controls the aggregation of α-synuclein by reducing the monomeric and aggregated forms of α-synuclein: involvement of the E3 ubiquitin ligase NEDD4-1. Cell Death Dis 13:575 (2022). PubMed: 35773260
- Moreno R et al. The stress-responsive kinase DYRK2 activates heat shock factor 1 promoting resistance to proteotoxic stress. Cell Death Differ 28:1563-1578 (2021). PubMed: 33268814
- Mazloumi Gavgani F et al. Nuclear Phosphatidylinositol 3,4,5-Trisphosphate Interactome Uncovers an Enrichment in Nucleolar Proteins. Mol Cell Proteomics 20:100102 (2021). PubMed: 34048982