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RecombinantRabMAb

Recombinant HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab209473)

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Western blot - HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab209473)
  • Western blot - HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab209473)
  • HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab209473)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR16786] to HNF-4-alpha - N-terminal
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Conjugation: HRP

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 594 Carrier Free Unconjugated

Overview

  • Product name

    HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal
    See all HNF-4-alpha primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR16786] to HNF-4-alpha - N-terminal

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2 whole cell and Mouse Liver tissue lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab209473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 47, 53 kDa (predicted molecular weight: 53 kDa).
Notes
WB
1/1000. Detects a band of approximately 47, 53 kDa (predicted molecular weight: 53 kDa).

Target

  • Function

    Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.

  • Involvement in disease

    Defects in HNF4A are the cause of maturity-onset diabetes of the young type 1 (MODY1) [MIM:125850]; also symbolized MODY-1. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.

  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • Post-translational
    modifications

    Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • FLJ39654 antibody
    • FRTS4 antibody
    • Hepatic nuclear factor 4 alpha antibody
    • Hepatocyte nuclear factor 4 alpha antibody
    • Hepatocyte nuclear factor 4 antibody
    • Hepatocyte nuclear factor 4-alpha antibody
    • HNF 4 alpha antibody
    • HNF 4 antibody
    • HNF-4-alpha antibody
    • HNF4 antibody
    • HNF4A antibody
    • HNF4A_HUMAN antibody
    • HNF4a7 antibody
    • HNF4a8 antibody
    • HNF4a9 antibody
    • Hnf4alpha antibody
    • HNF4alpha10/11/12 antibody
    • MODY 1 antibody
    • MODY antibody
    • MODY1 antibody
    • NR2A1 antibody
    • NR2A21 antibody
    • Nuclear receptor subfamily 2 group A member 1 antibody
    • OTTHUMP00000031060 antibody
    • OTTHUMP00000031062 antibody
    • TCF 14 antibody
    • TCF antibody
    • TCF-14 antibody
    • TCF14 antibody
    • Tcf4 antibody
    • Transcription factor 14, hepatic nuclear factor antibody
    • Transcription factor 14 antibody
    • Transcription factor HNF 4 antibody
    • Transcription factor HNF-4 antibody
    • Transcription factor HNF4 antibody
    see all

Images

  • Western blot - HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab209473)
    Western blot - HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab209473)
    All lanes : HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab209473) at 1/1000 dilution

    Lane 1 : HepG2 Whole Cell Lysate
    Lane 2 : Mouse Liver Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 47,53 kDa why is the actual band size different from the predicted?


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk/1x TBST blocking buffer before being incubated with ab209473 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • Western blot - HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab209473)
    Western blot - HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab209473)
    All lanes : HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab209473) at 1/1000 dilution

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 47,53 kDa why is the actual band size different from the predicted?


    Exposure time: 16 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209473 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab209473)
    HRP Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab209473)

References (0)

Publishing research using ab209473? Please let us know so that we can cite the reference in this datasheet.

ab209473 has not yet been referenced specifically in any publications.

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