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  1. Link

    products/primary-antibodies/hrp-muscle-actin-antibody-epr8484-loading-control-ab185058.pdf

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Tags & Cell Markers Subcellular Markers Cytoskeleton Actin
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RecombinantRabMAb

Recombinant HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (1) Submit a question References (1)

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Western blot - HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058)
  • HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR8484] to muscle Actin - Loading Control
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Conjugation: HRP

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    HRP Anti-muscle Actin antibody [EPR8484] - Loading Control
    See all muscle Actin primary antibodies
  • Description

    HRP Rabbit monoclonal [EPR8484] to muscle Actin - Loading Control
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human muscle Actin aa 350-450. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P68032

  • Positive control

    • NIH 3T3 cell lysate; Human fetal artery, kidney and heart lisates; Human uterus, stomach and skeletal muscle lysates; Human skeletal muscle tissue; Human smooth muscle in colon tissue; Human heart muscle tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin 300 Solution
    Constituents: 30% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8484
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Troponin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Tropomyosin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Crosslinking
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • RIP
    • Isotype/Loading Controls
    • Loading Controls
    • Actin
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • RIP

Associated products

  • Alternative Versions

    • Anti-muscle Actin antibody [EPR8484] (ab156302)
    • Alexa Fluor® 488 Anti-muscle Actin antibody [EPR8484] (ab190196)
    • Alexa Fluor® 647 Anti-muscle Actin antibody [EPR8484] (ab190567)
    • Anti-muscle Actin antibody [EPR8484] - BSA and Azide free (ab224207)
  • Isotype control

    • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)
  • Positive Controls

    • NIH/3T3 whole cell lysate (ab7179)
  • Related Products

    • Wiskostatin, N-WASP inhibitor (ab141085)
    • CK 666, Actin polymerization inhibitor (ab141231)
    • Jasplakinolide, Actin polymerization and stabilization inducer (ab141409)
    • Cytochalasin E, actin-polymerization inhibitor (ab141788)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab185058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
Notes
WB
1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy, actin, congenital, with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    Oxidation of Met-46 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68133 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 58 Human
    • Entrez Gene: 11459 Mouse
    • Entrez Gene: 29437 Rat
    • Omim: 102610 Human
    • SwissProt: P68133 Human
    • SwissProt: P68033 Mouse
    • SwissProt: P68134 Mouse
    • SwissProt: P68136 Rat
    • Unigene: 1288 Human
    • Unigene: 214950 Mouse
    • Unigene: 82732 Rat
    see all
  • Alternative names

    • a actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • ACTA2 antibody
    • ACTC antibody
    • ACTC1 antibody
    • Actin antibody
    • ACTS_HUMAN antibody
    • ACTSA antibody
    • Alpha 2 actin antibody
    • alpha skeletal muscle antibody
    • Alpha-actin-1 antibody
    • Cardiac muscle alpha actin 1 antibody
    • Skeletal muscle alpha actin 1 antibody
    see all

Images

  • Western blot - HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058)
    Western blot - HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058)
    All lanes : HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058) at 1/10000 dilution

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg
    Lane 2 : Human kidney tissue lysate - total protein (ab30203) at 20 µg
    Lane 3 : Human Fetal Heart at 5 µg
    Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
    Lane 5 : Skeletal Muscle (Rat) Tissue Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 5 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab185058 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406

  • HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058)
    HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (1)

Publishing research using ab185058? Please let us know so that we can cite the reference in this datasheet.

ab185058 has been referenced in 1 publication.

  • Zauri M  et al. CDA directs metabolism of epigenetic nucleosides revealing a therapeutic window in cancer. Nature 524:114-8 (2015). PubMed: 26200337

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Western blot abreview for Anti-Actin antibody [EPR8484] - Loading Control (HRP)

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
10000 cells
Gel Running Conditions
Reduced Denaturing
Sample
Arabidopsis thaliana Tissue lysate - whole (leaf)
Specification
leaf
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 07 2014

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