Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058)

Reviews (1) Submit a question References (1)
Western blot - HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058)
  • HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR8484] to muscle Actin - Loading Control
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Conjugation: HRP

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free Unconjugated

Overview

  • Product name

    HRP Anti-muscle Actin antibody [EPR8484] - Loading Control
    See all muscle Actin primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR8484] to muscle Actin - Loading Control

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human muscle Actin aa 350-450. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P68032

  • Positive control

    • NIH 3T3 cell lysate; Human fetal artery, kidney and heart lisates; Human uterus, stomach and skeletal muscle lysates; Human skeletal muscle tissue; Human smooth muscle in colon tissue; Human heart muscle tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab185058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
Notes
WB
1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

  • Involvement in disease

    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy, actin, congenital, with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.

  • Sequence similarities

    Belongs to the actin family.

  • Post-translational
    modifications

    Oxidation of Met-46 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.

  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • a actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • ACTA2 antibody
    • ACTC antibody
    • ACTC1 antibody
    • Actin antibody
    • ACTS_HUMAN antibody
    • ACTSA antibody
    • Alpha 2 actin antibody
    • alpha skeletal muscle antibody
    • Alpha-actin-1 antibody
    • Cardiac muscle alpha actin 1 antibody
    • Skeletal muscle alpha actin 1 antibody
    see all

Images

  • Western blot - HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058)
    Western blot - HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058)
    All lanes : HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058) at 1/10000 dilution

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg
    Lane 2 : Human kidney tissue lysate - total protein (ab30203) at 20 µg
    Lane 3 : Human Fetal Heart at 5 µg
    Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
    Lane 5 : Skeletal Muscle (Rat) Tissue Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 5 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab185058 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406

  • HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058)
    HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058)

References (1)

Publishing research using ab185058? Please let us know so that we can cite the reference in this datasheet.

ab185058 has been referenced in 1 publication.

  • Zauri M  et al. CDA directs metabolism of epigenetic nucleosides revealing a therapeutic window in cancer. Nature 524:114-8 (2015). PubMed: 26200337

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Western blot abreview for Anti-Actin antibody [EPR8484] - Loading Control (HRP)

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
10000 cells
Gel Running Conditions
Reduced Denaturing
Sample
Arabidopsis thaliana Tissue lysate - whole (leaf)
Specification
leaf
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 07 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com