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HRP Anti-Myc tag antibody [9E10] (ab62928)

Reviews (2)Q&A (1)References (30)
Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)
  • Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)

Key features and details

  • HRP Mouse monoclonal [9E10] to Myc tag
  • Suitable for: ELISA, WB
  • Reacts with: Human
  • Conjugation: HRP
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 APC Biotin Biotin DyLight® 488 DyLight® 550 DyLight® 650 FITC PE SureLight® APC Unconjugated

Overview

  • Product name

    HRP Anti-Myc tag antibody [9E10]
    See all Myc tag primary antibodies

  • Description

    HRP Mouse monoclonal [9E10] to Myc tag

  • Host species

    Mouse

  • Conjugation

    HRP

  • Tested applications

    Suitable for: ELISA, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to Human Myc tag aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.

  • Positive control

    • WB: E.coli Whole Cell Lysate (positive control) expressing 11 different epitope tags (ab5395) and Recombinant E.coli Multi Tag protein (ab36791).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab62928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
1/100 - 1/500.
WB (2)
1/10000 - 1/50000. Detects a band of approximately 41,45 kDa (predicted molecular weight: 49 kDa).
Notes
ELISA
1/100 - 1/500.
WB
1/10000 - 1/50000. Detects a band of approximately 41,45 kDa (predicted molecular weight: 49 kDa).

Target

  • Relevance

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

  • Cellular localization

    Nuclear

  • Database links

    • Alternative names

      • c-myc tag antibody
      • Myc Epitope Tag antibody

    Images

    • Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)
      Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)
      HRP Anti-Myc tag antibody [9E10] (ab62928) at 1/10000 dilution + Recombinant E. coli Multi Tag protein (ab36791) at 0.5 µg

      Predicted band size: 49 kDa
      Observed band size: 45 kDa why is the actual band size different from the predicted?


      Exposure time: 4 minutes


      Blocking buffer: 2% BSA
      Gel type: MOPS

    • Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)
      Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)
      HRP Anti-Myc tag antibody [9E10] (ab62928) at 1/10000 dilution + E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg

      Performed under reducing conditions.

      Predicted band size: 49 kDa
      Observed band size: 41 kDa why is the actual band size different from the predicted?

    References (30)

    Publishing research using ab62928? Please let us know so that we can cite the reference in this datasheet.

    ab62928 has been referenced in 30 publications.

    • Daoud M  et al. XIAP promotes melanoma growth by inducing tumour neutrophil infiltration. EMBO Rep 23:e53608 (2022). PubMed: 35437868
    • He W  et al. Targeted demethylation at ZNF154 promotor upregulates ZNF154 expression and inhibits the proliferation and migration of Esophageal Squamous Carcinoma cells. Oncogene 41:4537-4546 (2022). PubMed: 36064578
    • Jin JO  et al. Sequential ubiquitination of p53 by TRIM28, RLIM, and MDM2 in lung tumorigenesis. Cell Death Differ 28:1790-1803 (2021). PubMed: 33328571
    • Shailesh H  et al. Protein arginine methyltransferase 5 (PRMT5) activates WNT/ß-catenin signalling in breast cancer cells via epigenetic silencing of DKK1 and DKK3. J Cell Mol Med 25:1583-1600 (2021). PubMed: 33462997
    • Cho JH  et al. Engineering advanced logic and distributed computing in human CAR immune cells. Nat Commun 12:792 (2021). PubMed: 33542232
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-c-Myc antibody [9E10] (HRP)

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Escherichia coli Recombinant protein (BL21(DE3))
    Loading amount
    0.5 µg
    Specification
    BL21(DE3)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 30 2010

    Western blot abreview for Anti-c-Myc antibody [9E10] (HRP)

     Excellent
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T cells)
    Loading amount
    20 µg
    Specification
    293T cells
    Gel Running Conditions
    Reduced Denaturing (Invitrogen NUPAGE Bis-Tris Gel 4-12% Gradient Gel MES Buffer)
    Blocking step
    Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 25 2010

    Question

    Do you have information on the sensitivity of your anti-Myc-tag antibody? What is the minimal amount of protein I need to load onto a gel to detect my Myc-tagged fusion protein?

    Read More
    Answer

    The laboratory confirmed that they tested 0.1ug E. coli with myc-tag in WB and they obtained a good signal at 1 min exposure. However, they did not test the limits of ab62928 products sensitivity, but they would expect it to be able to detect smaller quantities than they have used.


    Read More

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