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    products/primary-antibodies/hrp-pd-l1-antibody-28-8-ab209961.pdf

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RecombinantRabMAb

Recombinant HRP Anti-PD-L1 antibody [28-8] (ab209961)

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  • Certificate of Compliance
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Western blot - HRP Anti-PD-L1 antibody [28-8] (ab209961)
  • HRP Anti-PD-L1 antibody [28-8] (ab209961)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [28-8] to PD-L1
  • Suitable for: WB
  • Reacts with: Human
  • Conjugation: HRP

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free FITC PE Unconjugated

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HRP Conjugation Kit - Lightning-Link® (ab102890)
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Recombinant human PD-L1 protein (Active) (ab167713)
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Anti-PD-L1 antibody [73-10] (ab228415)

View more associated products

Overview

  • Product name

    HRP Anti-PD-L1 antibody [28-8]
    See all PD-L1 primary antibodies
  • Description

    HRP Rabbit monoclonal [28-8] to PD-L1
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human PD-L1 (extracellular). The immunogen contains the specific extracellular domain of huPD-L1 (Phe19-Thr239). See reference for more info - www.ncbi.nlm.nih.gov/pmc/articles/PMC4561627/
    Database link: Q9NZQ7

  • Positive control

    • WB: CHO-PD-L1 whole cell lysate.
  • General notes

    Anti-PD-L1 antibody [28-8] has been used as detector antibody in Human PD-L1 SimpleStep ELISA® kit (ab214565).

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin 300 Solution
    Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    28-8
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Non-lineage
    • Immunology
    • Immune System Diseases
    • Autoimmune
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens
    • Cancer
    • Tumor biomarkers
    • Tumor antigens

Associated products

  • Alternative Versions

    • Anti-PD-L1 antibody [28-8] (ab205921)
    • APC Anti-PD-L1 antibody [28-8] (ab206967)
    • Anti-PD-L1 antibody [28-8] - Low endotoxin, Azide free (ab209889)
    • Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959)
    • Alexa Fluor® 647 Anti-PD-L1 antibody [28-8] - Extracellular domain (ab209960)
    • PE Anti-PD-L1 antibody [28-8] (ab209962)
    • Alexa Fluor® 555 Anti-PD-L1 antibody [28-8] (ab213358)
    • Alexa Fluor® 568 Anti-PD-L1 antibody [28-8] (ab213359)
    • Alexa Fluor® 594 Anti-PD-L1 antibody [28-8] (ab213360)
    • FITC Anti-PD-L1 antibody [28-8] (ab224027)
    • Anti-PD-L1 antibody [28-8] - BSA and Azide free (ab228413)
  • Isotype control

    • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)
  • Recombinant Protein

    • Recombinant human PD-L1 protein (Active) (ab167713)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab209961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 33 kDa.
Notes
WB
1/1000. Predicted molecular weight: 33 kDa.

Target

  • Function

    Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
  • Tissue specificity

    Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane and Endomembrane system.
  • Target information above from: UniProt accession Q9NZQ7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 29126 Human
    • Omim: 605402 Human
    • SwissProt: Q9NZQ7 Human
    • Unigene: 521989 Human
    • Alternative names

      • B7 H antibody
      • B7 H1 antibody
      • B7 homolog 1 antibody
      • B7-H1 antibody
      • B7H antibody
      • B7H1 antibody
      • CD 274 antibody
      • CD-274 antibody
      • CD274 antibody
      • CD274 antigen antibody
      • CD274 molecule antibody
      • MGC142294 antibody
      • MGC142296 antibody
      • OTTHUMP00000021029 antibody
      • PD L1 antibody
      • PD-L1 antibody
      • PD1L1_HUMAN antibody
      • PDCD1 ligand 1 antibody
      • PDCD1L1 antibody
      • PDCD1LG1 antibody
      • PDL 1 antibody
      • PDL1 antibody
      • Programmed cell death 1 ligand 1 antibody
      • Programmed death ligand 1 antibody
      • RGD1566211 antibody
      see all

    Images

    • Western blot - HRP Anti-PD-L1 antibody [28-8] (ab209961)
      Western blot - HRP Anti-PD-L1 antibody [28-8] (ab209961)
      All lanes : HRP Anti-PD-L1 antibody [28-8] (ab209961) at 1/1000 dilution

      Lane 1 : CHO-S
      Lane 2 : CHO-PD-L1

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 33 kDa
      Observed band size: 40-60 kDa why is the actual band size different from the predicted?


      Exposure time: 20 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209961 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • HRP Anti-PD-L1 antibody [28-8] (ab209961)
      HRP Anti-PD-L1 antibody [28-8] (ab209961)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab209961? Please let us know so that we can cite the reference in this datasheet.

    ab209961 has not yet been referenced specifically in any publications.

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