Recombinant HRP Anti-TRIM25/EFP antibody [EPR7315] (ab200788)
![Western blot - HRP Anti-TRIM25/EFP antibody [EPR7315] (ab200788)](https://www.abcam.com/ps/products/200/ab200788/Images/ab200788-248450-WBab2007881.jpg "Western blot - HRP Anti-TRIM25/EFP antibody [EPR7315] (ab200788)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR7315] to TRIM25/EFP
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
Overview
-
Product name
HRP Anti-TRIM25/EFP antibody [EPR7315]
See all TRIM25/EFP primary antibodies -
Description
HRP Rabbit monoclonal [EPR7315] to TRIM25/EFP -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: K562 whole cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7315 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
|
| Notes |
|---|
|
WB
1/5000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa). |
Target
-
Function
Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. -
Tissue specificity
Ubiquitous. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger. -
Domain
The RING-type zinc finger is important for ISG15 E3 ligase activity and autoISGylation. AutoISGylation negatively regulates ISG15 E3 ligase activity.
The C-terminal B30.2/SPRY domain interacts with the first N-terminal CARD domain of DDX58. -
Post-translational
modificationsAuto-ISGylated. -
Cellular localization
Cytoplasm. Colocalized with DDX58 at cytoplasmic perinuclear bodies. - Information by UniProt
-
Database links
- Entrez Gene: 7706 Human
- Entrez Gene: 217069 Mouse
- Entrez Gene: 494338 Rat
- Omim: 600453 Human
- SwissProt: Q14258 Human
- SwissProt: Q61510 Mouse
- Unigene: 528952 Human
- Unigene: 248445 Mouse
-
Alternative names
- E3 ubiquitin/ISG15 ligase TRIM25 antibody
- EFP antibody
- Estrogen responsive finger protein antibody
see all
Images
-
Western blot - HRP Anti-TRIM25/EFP antibody [EPR7315] (ab200788)HRP Anti-TRIM25/EFP antibody [EPR7315] (ab200788) at 1/5000 dilution + K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 71 kDa
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab200788 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
-
![HRP Anti-TRIM25/EFP antibody [EPR7315] (ab200788)](https://www.abcam.com/ps/products/200/ab200788/Images/ab200788-3-benefits-of-recombinant-antibodies.png)
HRP Anti-TRIM25/EFP antibody [EPR7315] (ab200788)
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab200788 has been referenced in 1 publication.
- Koliopoulos MG et al. Molecular mechanism of influenza A NS1-mediated TRIM25 recognition and inhibition. Nat Commun 9:1820 (2018). PubMed: 29739942