HRP Anti-Tubulin antibody [YL1/2] - Loading Control (ab197740)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Tubulin antibody [YL1/2] - Loading Control (ab197740)](https://www.abcam.com/ps/products/197/ab197740/Images/ab197740-248592-anti-tubulin-antibody-yl1-2-loading-control-hrp-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Tubulin antibody [YL1/2] - Loading Control (ab197740)")
Key features and details
- HRP Rat monoclonal [YL1/2] to Tubulin - Loading Control
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
- Isotype: IgG2a
Related conjugates and formulations
Overview
-
Product name
HRP Anti-Tubulin antibody [YL1/2] - Loading Control
See all Tubulin primary antibodies -
Description
HRP Rat monoclonal [YL1/2] to Tubulin - Loading Control -
Host species
Rat -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe, a wide range of other species, Mammals, African green monkey
-
Immunogen
Full length native protein (purified) corresponding to Saccharomyces cerevisiae Tubulin.
-
Epitope
A linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). -
Positive control
- WB: HeLa, NIH 3T3 and PC12 whole cell lysates. IHC-P: Normal human colon tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
YL1/2 -
Isotype
IgG2a -
Research areas
Associated products
-
Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500.
|
|
| WB |
1/5000. Detects a band of approximately 52 kDa.
|
| Notes |
|---|
|
IHC-P
1/500. |
|
WB
1/5000. Detects a band of approximately 52 kDa. |
Target
-
Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. -
Sequence similarities
Belongs to the tubulin family. -
Post-translational
modificationsUndergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
-
Database links
- Entrez Gene: 10376 Human
- Entrez Gene: 203068 Human
- Entrez Gene: 7280 Human
- Entrez Gene: 22143 Mouse
- Entrez Gene: 22151 Mouse
- Entrez Gene: 22153 Mouse
- Entrez Gene: 500929 Rat
- Omim: 191130 Human
see all -
Alternative names
- Tubulin beta 2b antibody
- Alpha tubulin antibody
- Alpha-tubulin ubiquitous antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Tubulin antibody [YL1/2] - Loading Control (ab197740)
IHC image of Tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab197740, 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
-
![Western blot - HRP Anti-Tubulin antibody [YL1/2] - Loading Control (ab197740)](https://www.abcam.com/ps/products/197/ab197740/Images/ab197740-246649-anti-tubulin-antibody-yl1-2-loading-control-hrp-western-blot.jpg)
Western blot - HRP Anti-Tubulin antibody [YL1/2] - Loading Control (ab197740)All lanes : HRP Anti-Tubulin antibody [YL1/2] - Loading Control (ab197740) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197740 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab197740 has been referenced in 1 publication.
- Amirnasr A et al. Expression and inhibition of BRD4, EZH2 and TOP2A in neurofibromas and malignant peripheral nerve sheath tumors. PLoS One 12:e0183155 (2017). WB . PubMed: 28813519