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Anti-HSD3B2 antibody [373CT9.1.3] (ab75710)

Reviews (2) Submit a question References (10)
Western blot - Anti-HSD3B2 antibody [373CT9.1.3] (ab75710)
  • Flow Cytometry - Anti-HSD3B2 antibody [373CT9.1.3] (ab75710)

Key features and details

  • Mouse monoclonal [373CT9.1.3] to HSD3B2
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-HSD3B2 antibody [373CT9.1.3]
    See all HSD3B2 primary antibodies

  • Description

    Mouse monoclonal [373CT9.1.3] to HSD3B2

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Goat, Horse, Cow, Cat, Dog, Pig, Cynomolgus monkey, Macaque monkey, Rhesus monkey

  • Immunogen

    Synthetic peptide corresponding to Human HSD3B2 aa 1-100 conjugated to keyhole limpet haemocyanin.

  • Positive control

    • This antibody gave a positive signal in human adrenal tissue lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab75710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 42 kDa). Abcam recommends blocking using 3% milk.
Flow Cyt
Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 42 kDa). Abcam recommends blocking using 3% milk.
Flow Cyt
Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

  • Tissue specificity

    Expressed in adrenal gland, testis and ovary.

  • Pathway

    Lipid metabolism; steroid biosynthesis.

  • Involvement in disease

    Defects in HSD3B2 are the cause of adrenal hyperplasia type 2 (AH2) [MIM:201810]. AH2 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: 'salt wasting' (SW, the most severe type), 'simple virilizing' (SV, less severely affected patients), with normal aldosterone biosynthesis, 'non-classic form' or late onset (NC or LOAH), and 'cryptic' (asymptomatic). In AH2, virilization is much less marked or does not occur. AH2 is frequently lethal in early life.
    Note=Mild HSD3B2 deficiency in hyperandrogenic females is associated with characteristic traits of polycystic ovary syndrome, such as insulin resistance and luteinizing hormon hypersecretion.

  • Sequence similarities

    Belongs to the 3-beta-HSD family.

  • Cellular localization

    Endoplasmic reticulum membrane. Mitochondrion membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 3 beta HSD adrenal and gonadal type antibody
    • 3 beta HSD II antibody
    • 3 beta HSD type II antibody
    • 3 beta hydroxy 5 ene steroid dehydrogenase antibody
    • 3 beta hydroxy Delta(5) steroid dehydrogenase antibody
    • 3 beta hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 antibody
    • 3 beta-hydroxysteroid dehydrogenase type II, delta 5-delta 4-isomerase type II, 3 beta-HSD type II antibody
    • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II antibody
    • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 antibody
    • 3-beta-HSD II antibody
    • 3-beta-hydroxy-5-ene steroid dehydrogenase antibody
    • 3-beta-hydroxy-Delta(5)-steroid dehydrogenase antibody
    • 3BHS2_HUMAN antibody
    • ADRENAL HYPERPLASIA II antibody
    • beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 antibody
    • delta 5 delta 4 isomerase type II antibody
    • Delta-5-3-ketosteroid isomerase antibody
    • HSD3B antibody
    • HSD3B2 antibody
    • HSDB antibody
    • HSDB3B antibody
    • hydroxy delta 5 steroid dehydrogenase, 3 beta and steroid delta isomerase 2 antibody
    • Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 antibody
    • Progesterone reductase antibody
    • SDR11E2 antibody
    • short chain dehydrogenase/reductase family 11E, member 2 antibody
    • Steroid Delta-isomerase antibody
    see all

Images

  • Western blot - Anti-HSD3B2 antibody [373CT9.1.3] (ab75710)
    Western blot - Anti-HSD3B2 antibody [373CT9.1.3] (ab75710)
    Anti-HSD3B2 antibody [373CT9.1.3] (ab75710) at 1 µg/ml + Human adrenal normal tissue lysate - total protein (ab29249) at 20 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?
    Additional bands at: 110 kDa, 35 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    Blocked using 3% milk.
  • Flow Cytometry - Anti-HSD3B2 antibody [373CT9.1.3] (ab75710)
    Flow Cytometry - Anti-HSD3B2 antibody [373CT9.1.3] (ab75710)
    Overlay histogram showing JEG3 cells stained with ab75710 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75710, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References (10)

Publishing research using ab75710? Please let us know so that we can cite the reference in this datasheet.

ab75710 has been referenced in 10 publications.

  • Wang J  et al. H2 S catalysed by CBS regulates testosterone synthesis through affecting the sulfhydrylation of PDE. J Cell Mol Med 25:3460-3468 (2021). PubMed: 33713531
  • Li M  et al. Quercetin alleviated H2 O2 -induced apoptosis and steroidogenic impairment in goat luteinized granulosa cells. J Biochem Mol Toxicol N/A:e22527 (2020). PubMed: 32410385
  • Kurowska P  et al. Role of vaspin in porcine ovary: effect on signaling pathways and steroid synthesis via GRP78 receptor and protein kinase A†. Biol Reprod 102:1290-1305 (2020). PubMed: 32149334
  • Maoduo Z  et al. Effects of LPS on the accumulation of lipid droplets, proliferation, and steroidogenesis in goat luteinized granulosa cells. J Biochem Mol Toxicol N/A:e22329 (2019). PubMed: 30934154
  • Ide H  et al. Modulation of AKR1C2 by curcumin decreases testosterone production in prostate cancer. Cancer Sci 109:1230-1238 (2018). WB ; Human . PubMed: 29369461
  • Neubauer E  et al. Up regulation of the steroid hormone synthesis regulator HSD3B2 is linked to early PSA recurrence in prostate cancer. Exp Mol Pathol 105:50-56 (2018). PubMed: 29803408
  • Fadhillah  et al. Hypoxia-inducible factor 1 mediates hypoxia-enhanced synthesis of progesterone during luteinization of granulosa cells. J Reprod Dev 63:75-85 (2017). WB ; Cow . PubMed: 27840375
  • Fadhillah  et al. Hypoxia promotes progesterone synthesis during luteinization in bovine granulosa cells. J Reprod Dev 60:194-201 (2014). PubMed: 24583842
  • Abe H  et al. Lymphatic involvement in the disappearance of steroidogenic cells from the corpus luteum during luteolysis. PLoS One 9:e88953 (2014). IHC ; Cow . PubMed: 24586455
  • Kozai K  et al. Expression of aldo-keto reductase 1C23 in the equine corpus luteum in different luteal phases. J Reprod Dev 60:150-4 (2014). WB ; Horse . PubMed: 24492656

Customer reviews and Q&As

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Western blot abreview for Anti-HSD3B2 antibody [373CT9.1.3]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Dog Tissue lysate - other (adrenal, testes)
Gel Running Conditions
Reduced Denaturing (BioRad Criterion TGX 10%)
Loading amount
20 µg
Specification
adrenal, testes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 02 2020

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