Anti-HSDL2 antibody [2A11BG6AH6] (ab110298)
Key features and details
- Mouse monoclonal [2A11BG6AH6] to HSDL2
- Suitable for: ICC, IP
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-HSDL2 antibody [2A11BG6AH6]
See all HSDL2 primary antibodies -
Description
Mouse monoclonal [2A11BG6AH6] to HSDL2 -
Host species
Mouse -
Tested applications
Suitable for: ICC, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Hela cell and human liver mitochondria.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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Purity
Ammonium Sulphate Precipitation -
Purification notes
Purity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation -
Clonality
Monoclonal -
Clone number
2A11BG6AH6 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC |
Use a concentration of 10 µg/ml.
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|
IP |
Use at an assay dependent concentration.
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Notes |
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ICC
Use a concentration of 10 µg/ml. |
IP
Use at an assay dependent concentration. |
Target
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Function
Has apparently no steroid dehydrogenase activity. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Contains 1 SCP2 domain. -
Cellular localization
Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 84263 Human
- SwissProt: Q6YN16 Human
- Unigene: 59486 Human
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Alternative names
- C9orf99 antibody
- FLJ25855 antibody
- hsdl2 antibody
see all
Images
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As an IP antibody, HSDL2 antibody [2A11BG6AH6 ] (ab110298) works with human sample only.
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Immunocytochemistry image of HSDL2 stained human Hela cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with HSDL2 antibody [2A11BG6AH6 ](ab110298) at 10 µg/ml for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab110298 has not yet been referenced specifically in any publications.