Recombinant Anti-HSDL2 antibody [EPR13876(B)] (ab181174)
![Western blot - Anti-HSDL2 antibody [EPR13876(B)] (ab181174)](https://www.abcam.com/ps/products/181/ab181174/Images/ab181174-214653-anti-hsdl2-antibody-epr13876b-western-blot.jpg "Western blot - Anti-HSDL2 antibody [EPR13876(B)] (ab181174)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13876(B)] to HSDL2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-HSDL2 antibody [EPR13876(B)]
See all HSDL2 primary antibodies -
Description
Rabbit monoclonal [EPR13876(B)] to HSDL2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, 293 and Human fetal liver lysates, Human kidney tissue, HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13876(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| ICC/IF |
1/50 - 1/100.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 45 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
1/50 - 1/100. |
Target
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Function
Has apparently no steroid dehydrogenase activity. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Contains 1 SCP2 domain. -
Cellular localization
Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 84263 Human
- SwissProt: Q6YN16 Human
- Unigene: 59486 Human
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Alternative names
- C9orf99 antibody
- FLJ25855 antibody
- hsdl2 antibody
see all
Images
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Western blot - Anti-HSDL2 antibody [EPR13876(B)] (ab181174)All lanes : Anti-HSDL2 antibody [EPR13876(B)] (ab181174) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-HSDL2 antibody [EPR13876(B)] (ab181174)](https://www.abcam.com/ps/products/181/ab181174/Images/ab181174-214656-anti-hsdl2-antibody-epr13876b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HSDL2 antibody [EPR13876(B)] (ab181174)Immunofluorescent staining of -20℃ Acetone fixed HeLa cells labeling HSDL2 using ab181174 at a 1/100 dilution and a Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at a 1/200 dilution and counterstained with Dapi.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSDL2 antibody [EPR13876(B)] (ab181174)](https://www.abcam.com/ps/products/181/ab181174/Images/ab181174-214655-anti-hsdl2-antibody-epr13876b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSDL2 antibody [EPR13876(B)] (ab181174)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling HSDL2 using ab181174 at a 1/100 dilution using prediluted HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-HSDL2 antibody [EPR13876(B)] (ab181174)](https://www.abcam.com/ps/products/181/ab181174/Images/ab181174-214654-anti-hsdl2-antibody-epr13876b-western-blot.jpg)
Western blot - Anti-HSDL2 antibody [EPR13876(B)] (ab181174)Anti-HSDL2 antibody [EPR13876(B)] (ab181174) at 1/10000 dilution + Human fetal liver lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 45 kDa -
![Anti-HSDL2 antibody [EPR13876(B)] (ab181174)](https://www.abcam.com/ps/products/181/ab181174/Images/ab181174-5-benefits-of-recombinant-antibodies.png)
Anti-HSDL2 antibody [EPR13876(B)] (ab181174)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab181174 has been referenced in 1 publication.
- Sun Q et al. Role of Hydroxysteroid Dehydrogenase-Like 2 (HSDL2) in Human Ovarian Cancer. Med Sci Monit 24:3997-4008 (2018). PubMed: 29894468