Recombinant Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400)
![Western blot - Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400)](https://www.abcam.com/ps/products/250/ab250400/Images/ab250400-407817-anti-hsdl2-antibody-epr13876-b-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13876(B)] to HSDL2 - BSA and Azide free
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free
See all HSDL2 primary antibodies -
Description
Rabbit monoclonal [EPR13876(B)] to HSDL2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250400 is the carrier-free version of ab181174.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13876(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| ICC/IF |
Use at an assay dependent concentration.
|
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
|
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 45 kDa. |
Target
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Function
Has apparently no steroid dehydrogenase activity. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Contains 1 SCP2 domain. -
Cellular localization
Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 84263 Human
- SwissProt: Q6YN16 Human
- Unigene: 59486 Human
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Alternative names
- C9orf99 antibody
- FLJ25855 antibody
- hsdl2 antibody
see all
Images
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Western blot - Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400)All lanes : Anti-HSDL2 antibody [EPR13876(B)] (ab181174) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDaThis data was developed using ab181174, the same antibody clone in a different buffer formulation.
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![Immunocytochemistry/ Immunofluorescence - Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400)](https://www.abcam.com/ps/products/250/ab250400/Images/ab250400-3-anti-hsdl2-antibody-epr13876-b-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400)This data was developed using ab181174, the same antibody clone in a different buffer formulation.Immunofluorescent staining of -20? Acetone fixed HeLa cells labeling HSDL2 using ab181174 at a 1/100 dilution and a Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at a 1/200 dilution and counterstained with Dapi. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400)](https://www.abcam.com/ps/products/250/ab250400/Images/ab250400-2-anti-hsdl2-antibody-epr13876-b-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400)This data was developed using ab181174, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling HSDL2 using ab181174 at a 1/100 dilution using prediluted HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Western blot - Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400)](https://www.abcam.com/ps/products/250/ab250400/Images/ab250400-398661-anti-hsdl2-antibody-epr13876-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400) -
![Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400)](https://www.abcam.com/ps/products/250/ab250400/Images/ab250400-5-benefits-of-recombinant-antibodies.png)
Anti-HSDL2 antibody [EPR13876(B)] - BSA and Azide free (ab250400)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250400 has not yet been referenced specifically in any publications.
