Anti-HSP17.6 antibody (ab80183)
Key features and details
- Rabbit polyclonal to HSP17.6
- Suitable for: WB
- Reacts with: Arabidopsis thaliana, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-HSP17.6 antibody -
Description
Rabbit polyclonal to HSP17.6 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Arabidopsis thaliana, Recombinant fragment -
Immunogen
Full length recombinant HSP17.6 protein produced in E. coli and purified by conventional methods (no affinity tag)
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Positive control
- Heat shocked (38° C for 2 hours) Arabidopsis thaliana lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
(ECL) |
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). (ECL) |
Target
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Relevance
HSP17.6 belongs to the class I family of small heat shock proteins. Small Hsps are induced when plant cells are stressed by increased temperature and appear to be involved in chaperone functions by protecting other proteins from irreversible denaturation. They are produced late in the seed maturation process. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 831076 Arabidopsis thaliana
- SwissProt: P13853 Arabidopsis thaliana
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Alternative names
- 17.6 kDa class I heat shock protein antibody
- Arabidopsis thaliana heat shock protein 17.6A antibody
- AT HSP17.6A antibody
see all
Images
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Western blot - Anti-HSP17.6 antibody (ab80183)Lanes 1-2 & 4-7 : Anti-HSP17.6 antibody (ab80183) at 1/1000 dilution
Lane 1 : Total protein from heat shocked Arabidopsis thaliana at 15 µg
Lane 2 : Arabidopsis thaliana control plant
Lane 3 : Empty lane
Lane 4 : Recombinant purified HSP17.6, 1 ng
Lane 5 : Recombinant purified HSP17.6, 2 ng
Lane 6 : Lysates from Arabidopsis thaliana, 5 ng
Lane 7 : recombinant purified HSP17.6, 10 ng
Secondary
Lanes 1-2 & 4-7 : HRP-conjugated goat anti rabbit IgG
Predicted band size: 18 kDa15% SDS-PAGE and blotted 1hour onto nitrocellulose
Datasheets and documents
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Datasheet download
References (3)
ab80183 has been referenced in 3 publications.
- Yamaguchi N et al. H3K27me3 demethylases alter HSP22 and HSP17.6C expression in response to recurring heat in Arabidopsis. Nat Commun 12:3480 (2021). PubMed: 34108473
- Davies M et al. Stress in native grasses under ecologically relevant heat waves. PLoS One 13:e0204906 (2018). PubMed: 30308074
- Weng M et al. Histone chaperone ASF1 is involved in gene transcription activation in response to heat stress in Arabidopsis thaliana. Plant Cell Environ 37:2128-38 (2014). PubMed: 24548003
