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  1. Link

    products/primary-antibodies/hsp40-antibody-ab226387.pdf

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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
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Anti-Hsp40 antibody (ab226387)

  • Datasheet
  • SDS
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Western blot - Anti-Hsp40 antibody (ab226387)
  • Immunoprecipitation - Anti-Hsp40 antibody (ab226387)

Key features and details

  • Rabbit polyclonal to Hsp40
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Hsp40 antibody
    See all Hsp40 primary antibodies
  • Description

    Rabbit polyclonal to Hsp40
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Hsp40 aa 290-340. The exact sequence is proprietary.
    Database link: P25685

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab226387 was affinity purified using an epitope specific to Hsp40 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant E. coli Hsp40 protein (ab91598)
  • Related Products

    • Recombinant Human Hsp40 protein (ab172855)
    • Recombinant Human Hsp40 protein (ab43613)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab226387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 38 kDa.
IP
Use at 2-10 µg/mg of lysate.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 38 kDa.
IP
Use at 2-10 µg/mg of lysate.

Target

  • Function

    Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.
  • Sequence similarities

    Contains 1 J domain.
  • Cellular localization

    Cytoplasm. Nucleus. Nucleus > nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
  • Target information above from: UniProt accession P25685 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3337 Human
    • Omim: 604572 Human
    • SwissProt: P25685 Human
    • Unigene: 515210 Human
    • Alternative names

      • DnaJ (Hsp40) homolog subfmaily B member 1 antibody
      • DNAJ 1 antibody
      • DNAJ B1 antibody
      • DnaJ heat shock protein family (Hsp40) member B1 antibody
      • DnaJ homolog subfamily B member 1 antibody
      • DnaJ protein homolog 1 antibody
      • DNAJ1 antibody
      • DNAJB 1 antibody
      • Dnajb1 antibody
      • DNAJB1 protein antibody
      • DNJB1_HUMAN antibody
      • HDJ 1 antibody
      • HDJ-1 antibody
      • HDJ1 antibody
      • Heat shock 40 kDa protein 1 antibody
      • Heat shock 40kD protein 1 antibody
      • Heat shock protein 40 antibody
      • Hsp 40 antibody
      • HSP40 antibody
      • HSPF 1 antibody
      • HSPF1 antibody
      • Human DnaJ protein 1 antibody
      • Radial spoke 16 homolog B antibody
      • RSPH16B antibody
      • Sis1 antibody
      see all

    Images

    • Western blot - Anti-Hsp40 antibody (ab226387)
      Western blot - Anti-Hsp40 antibody (ab226387)
      All lanes : Anti-Hsp40 antibody (ab226387) at 0.4 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 38 kDa


      Exposure time: 3 minutes


      Lysates prepared using NETN lysis buffer.

    • Immunoprecipitation - Anti-Hsp40 antibody (ab226387)
      Immunoprecipitation - Anti-Hsp40 antibody (ab226387)

      Hsp40 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226387 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226387 at 1 µg/ml.

      Lane 1: ab226387 IP in HEK-293T whole cell lysate.

      Lane 2: Control IgG IP in HEK-293T whole cell lysate.

      Detection:  Chemiluminescence with exposure time of 3 minutes.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab226387? Please let us know so that we can cite the reference in this datasheet.

    ab226387 has not yet been referenced specifically in any publications.

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