Anti-Hsp40 antibody (ab226387)
Key features and details
- Rabbit polyclonal to Hsp40
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Hsp40 antibody
See all Hsp40 primary antibodies -
Description
Rabbit polyclonal to Hsp40 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Hsp40 aa 290-340. The exact sequence is proprietary.
Database link: P25685 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226387 was affinity purified using an epitope specific to Hsp40 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 38 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| Notes |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 38 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. -
Sequence similarities
Contains 1 J domain. -
Cellular localization
Cytoplasm. Nucleus. Nucleus > nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock. - Information by UniProt
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Database links
- Entrez Gene: 3337 Human
- Omim: 604572 Human
- SwissProt: P25685 Human
- Unigene: 515210 Human
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Alternative names
- DnaJ (Hsp40) homolog subfmaily B member 1 antibody
- DNAJ 1 antibody
- DNAJ B1 antibody
see all
Images
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Western blot - Anti-Hsp40 antibody (ab226387)All lanes : Anti-Hsp40 antibody (ab226387) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 38 kDa
Exposure time: 3 minutesLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-Hsp40 antibody (ab226387)Hsp40 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226387 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226387 at 1 µg/ml.
Lane 1: ab226387 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226387 has not yet been referenced specifically in any publications.