Anti-Hsp60 antibody [1D11BD8] - Mitochondrial Marker (ab110312)
![Western blot - Anti-Hsp60 antibody [1D11BD8] - Mitochondrial Marker (ab110312)](https://www.abcam.com/ps/products/110/ab110312/Images/ab110312-123591-anti-hsp60-antibody-1d11bd8-mitochondrial-marker-western-blot.gif "Western blot - Anti-Hsp60 antibody [1D11BD8] - Mitochondrial Marker (ab110312)")
Key features and details
- Mouse monoclonal [1D11BD8] to Hsp60 - Mitochondrial Marker
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Hsp60 antibody [1D11BD8] - Mitochondrial Marker
See all Hsp60 primary antibodies -
Description
Mouse monoclonal [1D11BD8] to Hsp60 - Mitochondrial Marker -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human heart tissue HepG2 Fibroblast cells HeLa cells Human normal colon FFPE tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
1D11BD8 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 5 µg/ml.
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| WB | (2) |
Use a concentration of 5 µg/ml. Predicted molecular weight: 61 kDa.
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| ICC/IF | (2) |
Use a concentration of 1 µg/ml.
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| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
IHC-P
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 5 µg/ml. Predicted molecular weight: 61 kDa. |
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ICC/IF
Use a concentration of 1 µg/ml. |
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Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. -
Involvement in disease
Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life. -
Sequence similarities
Belongs to the chaperonin (HSP60) family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 3329 Human
- Omim: 118190 Human
- SwissProt: P10809 Human
- Unigene: 595053 Human
- Unigene: 727543 Human
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Alternative names
- 60 kDa chaperonin antibody
- 60 kDa heat shock protein, mitochondrial antibody
- CH60_HUMAN antibody
see all
Images
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Western blot - Anti-Hsp60 antibody [1D11BD8] - Mitochondrial Marker (ab110312)Anti-Hsp60 antibody [1D11BD8] - Mitochondrial Marker (ab110312) at 5 µg/ml + HepG2 whole cells at 10 µg
Predicted band size: 61 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [1D11BD8] - Mitochondrial Marker (ab110312)](https://www.abcam.com/ps/products/110/ab110312/Images/ab110312-245071-anti-hsp60-antibody-1d11bd8-mitochondrial-marker-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [1D11BD8] - Mitochondrial Marker (ab110312)This image is courtesy of an anonymous Abreviewab110312 staining Hsp60 in MDA MB 231 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 1% Triton X-100 and blocked with 10% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/100 in BSA + 0.02% Tween20) for 1 hour at 16°C. A DyLight® 550-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [1D11BD8] - Mitochondrial Marker (ab110312)](https://www.abcam.com/ps/products/110/ab110312/Images/ab110312-123590-anti-hsp60-antibody-1d11bd8-mitochondrial-marker-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [1D11BD8] - Mitochondrial Marker (ab110312)Immunocytochemistry image of stained fibroblast cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the ab110312 antibody (1 µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent. -
![Flow Cytometry - Anti-Hsp60 antibody [1D11BD8] - Mitochondrial Marker (ab110312)](https://www.abcam.com/ps/products/110/ab110312/Images/ab110312-123589-anti-hsp60-antibody-1d11bd8-mitochondrial-marker-flow-cytometry.jpg)
Flow Cytometry - Anti-Hsp60 antibody [1D11BD8] - Mitochondrial Marker (ab110312)HeLa cells were stained with 1 µg/mL ab110312 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [1D11BD8] - Mitochondrial Marker (ab110312)](https://www.abcam.com/ps/products/110/ab110312/Images/ab110312-123588-anti-hsp60-antibody-1d11bd8-mitochondrial-marker-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [1D11BD8] - Mitochondrial Marker (ab110312)IHC image of Hsp60 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110312, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab110312 has been referenced in 4 publications.
- Bie AS et al. An inventory of interactors of the human HSP60/HSP10 chaperonin in the mitochondrial matrix space. Cell Stress Chaperones 25:407-416 (2020). PubMed: 32060690
- Koh CWQ et al. Atlas of quantitative single-base-resolution N6-methyl-adenine methylomes. Nat Commun 10:5636 (2019). PubMed: 31822664
- Liang XH et al. RNase H1-Dependent Antisense Oligonucleotides Are Robustly Active in Directing RNA Cleavage in Both the Cytoplasm and the Nucleus. Mol Ther 25:2075-2092 (2017). PubMed: 28663102
- Magnoni R et al. The Hsp60 folding machinery is crucial for manganese superoxide dismutase folding and function. Free Radic Res 48:168-79 (2014). PubMed: 24151936