Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker (ab128567)
![Western blot - Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker (ab128567)](https://www.abcam.com/ps/products/128/ab128567/Images/ab128567-142392-anti-hsp60-antibody-usc127-3-mitochondrial-marker-western-blot.jpg "Western blot - Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker (ab128567)")
Key features and details
- Mouse monoclonal [USC127-3] to Hsp60 - Mitochondrial Marker
- Suitable for: IHC-P, Flow Cyt, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker
See all Hsp60 primary antibodies -
Description
Mouse monoclonal [USC127-3] to Hsp60 - Mitochondrial Marker -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, Flow Cyt, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- HepG2, Hela, Jurkat, HDFn, Hek293T cell lysates; Human Heart Homogenate lysate; Human Liver mitochondrial lysate; HDFn cells; Hela cells
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Purity is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. -
Clonality
Monoclonal -
Clone number
USC127-3 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128567 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 0.2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Flow Cyt |
Use a concentration of 1 - 5 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa.
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| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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| Notes |
|---|
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IHC-P
Use a concentration of 0.2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
Flow Cyt
Use a concentration of 1 - 5 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa. |
|
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
Target
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Function
Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. -
Involvement in disease
Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life. -
Sequence similarities
Belongs to the chaperonin (HSP60) family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 3329 Human
- Omim: 118190 Human
- SwissProt: P10809 Human
- Unigene: 595053 Human
- Unigene: 727543 Human
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Alternative names
- 60 kDa chaperonin antibody
- 60 kDa heat shock protein, mitochondrial antibody
- CH60_HUMAN antibody
see all
Images
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Western blot - Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker (ab128567)All lanes : Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker (ab128567) at 1 µg/ml
Lane 1 : Ladder
Lane 2 : HepG2 cell lysate at 10 µg
Lane 3 : Hela cell lysate at 10 µg
Lane 4 : Jurkat cell lysate at 10 µg
Lane 5 : HDFn cell lysate at 10 µg
Lane 6 : Hek293T cell lysate at 10 µg
Lane 7 : Human Heart Homogenate lysate at 10 µg
Lane 8 : Human Liver mitochondrial lysate at 10 µg
Predicted band size: 61 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker (ab128567)](https://www.abcam.com/ps/products/128/ab128567/Images/ab128567-142391-anti-hsp60-antibody-usc127-3-mitochondrial-marker-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker (ab128567)Immunofluorescent staining of Hsp60 in HDFn cells (human) using ab128567.
The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were then incubated with the antibody (ab128567, 1µg/ml) for 2h at room temperature or overnight at 4°C. The secondary antibody was (Green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix. -
![Flow Cytometry - Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker (ab128567)](https://www.abcam.com/ps/products/128/ab128567/Images/ab128567-142390-anti-hsp60-antibody-usc127-3-mitochondrial-marker-flow-cytometry.jpg)
Flow Cytometry - Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker (ab128567)ab128567 staining Hsp60 in Hela cells by Flow cytometry.
Hela cells were fixed and permeabilized with methanol and stained with 5µg/mL Hsp60 antibody (ab128567) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry. 1% BSA was used as the blocking reagent for all the blocking steps. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker (ab128567)](https://www.abcam.com/ps/products/128/ab128567/Images/ab128567-142389-anti-hsp60-antibody-usc127-3-mitochondrial-marker-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [USC127-3] - Mitochondrial Marker (ab128567)IHC image of Hsp60 staining in human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab128567, 0.2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab128567 has been referenced in 2 publications.
- Nam SE et al. Insights on autophagosome-lysosome tethering from structural and biochemical characterization of human autophagy factor EPG5. Commun Biol 4:291 (2021). PubMed: 33674710
- Zhou Y et al. Topology-dependent, bifurcated mitochondrial quality control under starvation. Autophagy 16:562-574 (2020). PubMed: 31234709
