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RecombinantRabMAb

Recombinant Anti-Hsp70 antibody [EPR16893] (ab194360)

Reviews (1) Submit a question References (2)
Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [EPR16893] (ab194360)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody [EPR16893] (ab194360)
  • Flow Cytometry (Intracellular) - Anti-Hsp70 antibody [EPR16893] (ab194360)
  • Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)
  • Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)
  • Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)
  • Anti-Hsp70 antibody [EPR16893] (ab194360)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16893] to Hsp70
  • Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

Overview

  • Product name

    Anti-Hsp70 antibody [EPR16893]
    See all Hsp70 primary antibodies

  • Description

    Rabbit monoclonal [EPR16893] to Hsp70

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, HepG2, Jurkat, C6, PC12, human testis, mouse testis and rat testis lysates; Human endometrium tissue; A431 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab194360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/120.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB (1)
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
ICC/IF
1/150.
Notes
Flow Cyt (Intra)
1/120.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
ICC/IF
1/150.

Target

  • Relevance

    Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific.

  • Cellular localization

    Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

  • Database links

    see all

  • Alternative names

    • DnaK type molecular chaperone HSP70 1 antibody
    • Epididymis secretory protein Li 103 antibody
    • FLJ54303 antibody
    • FLJ54370 antibody
    • FLJ54392 antibody
    • FLJ54408 antibody
    • FLJ75127 antibody
    • Heat shock 70 kDa protein 1 antibody
    • Heat shock 70 kDa protein 1/2 antibody
    • Heat shock 70 kDa protein 1A antibody
    • Heat shock 70 kDa protein 1A/1B antibody
    • Heat shock 70kDa protein 1B antibody
    • Heat shock induced protein antibody
    • HEL S 103 antibody
    • HSP70 1 antibody
    • HSP70 1A antibody
    • HSP70 1B antibody
    • HSP70 2 antibody
    • HSP70-1 antibody
    • HSP70-1/HSP70-2 antibody
    • HSP70-1A antibody
    • HSP70-2 antibody
    • HSP70.1 antibody
    • HSP70.1/HSP70.2 antibody
    • HSP71_HUMAN antibody
    • HSP72 antibody
    • HSPA1 antibody
    • HSPA1A antibody
    • HSPA1B antibody
    see all

Images

  • Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)
    Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)
    All lanes : Anti-Hsp70 antibody [EPR16893] (ab194360) at 1/2000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : PC12 cell lysate
    Lane 3 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [EPR16893] (ab194360)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [EPR16893] (ab194360)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Hsp70 with ab194360 at 1/1000 (1.67μg/mL) dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody [EPR16893] (ab194360)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody [EPR16893] (ab194360)

    Immunofluorescent analysis of A431 cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling Hsp70 with ab194360 at 1/150 dilution (11 μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).

  • Flow Cytometry (Intracellular) - Anti-Hsp70 antibody [EPR16893] (ab194360)
    Flow Cytometry (Intracellular) - Anti-Hsp70 antibody [EPR16893] (ab194360)

    Intracellular Flow Cytometry analysis of HeLa cells labelling Hsp70 (red) with purified ab194360 at dilution of 1/120. The secondary antibody used was Alexa Fluorr® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody. 

     

     

  • Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)
    Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)
    All lanes : Anti-Hsp70 antibody [EPR16893] (ab194360) at 1/2000 dilution

    Lane 1 : Mouse testis tissue lysate
    Lane 2 : Rat testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 70 kDa

  • Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)
    Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)
    All lanes : Anti-Hsp70 antibody [EPR16893] (ab194360) at 1/20000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 70 kDa

  • Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)
    Western blot - Anti-Hsp70 antibody [EPR16893] (ab194360)
    All lanes : Anti-Hsp70 antibody [EPR16893] (ab194360) at 1/20000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 70 kDa

  • Anti-Hsp70 antibody [EPR16893] (ab194360)
    Anti-Hsp70 antibody [EPR16893] (ab194360)

References (2)

Publishing research using ab194360? Please let us know so that we can cite the reference in this datasheet.

ab194360 has been referenced in 2 publications.

  • Huang C  et al. Circulating plasma exosomal miRNA profiles serve as potential metastasis-related biomarkers for hepatocellular carcinoma. Oncol Lett 21:168 (2021). PubMed: 33552286
  • Wang H  et al. Reciprocal interaction between SIRT6 and APC/C regulates genomic stability. Sci Rep 11:14253 (2021). PubMed: 34244565

Customer reviews and Q&As

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Western blot abreview for Anti-Hsp70 antibody [EPR16893]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Liver Lysate)
Gel Running Conditions
Reduced Denaturing (4-12% gel)
Loading amount
40 µg
Specification
Liver Lysate
Blocking step
commercial blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted May 05 2021

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