Recombinant Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)
![Western blot - Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)](https://www.abcam.com/ps/products/282/ab282108/Images/ab282108-4-anti-hsp90-antibody-68hsp90-western-blot-wildtype-hek293t.jpg "Western blot - Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [68/Hsp90] to Hsp90 alpha
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Hsp90 alpha antibody [68/Hsp90]
See all Hsp90 alpha primary antibodies -
Description
Mouse monoclonal [68/Hsp90] to Hsp90 alpha -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HEK-293T,Jurkat and HeLa, K-562 lysates. ICC: 293T, HeLa cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
68/Hsp90 -
Isotype
IgG1 -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab282108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000.
|
|
| ICC/IF |
1/50.
|
| Notes |
|---|
|
WB
1/1000. |
|
ICC/IF
1/50. |
Target
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Function
Molecular chaperone. Has ATPase activity. -
Sequence similarities
Belongs to the heat shock protein 90 family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 3320 Human
- Omim: 140571 Human
- SwissProt: P07900 Human
- Unigene: 525600 Human
- Unigene: 700831 Human
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Alternative names
- EL52 antibody
- epididymis luminal secretory protein 52 antibody
- Heat shock 86 kDa antibody
see all
Images
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Western blot - Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)All lanes : Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : HSP90AA1 KO HEK293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (IRDye® 800CW) (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (ab216777) at 1/10000 dilution
Observed band size: 83 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS.
Lanes 1-3: Merged signal (red and green). Green - ab282108 observed at 83KDa. Red - loading control ab181602 (Rabbit monoclonal [EPR16891] to GAPDH) observed at 36 kDa.
Lanes 1-2: ab282108 Anti-Hsp90 alpha antibody was shown to react with Hsp90 alpha in HEK293T cells in Western blot. Loss of signal was observed when HSP90AA1 knockout sample was used. Wild-type and
HSP90AA1 knockout samples were subjected to SDS-PAGE.
ab282108 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated at 4? overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680CW) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800RD) preadsorbed (ab216772) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging. -
![Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)](https://www.abcam.com/ps/products/282/ab282108/Images/ab282108-1-anti-hsp90-antibody-68hsp90-immunocytochemistry-293t.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells labelling Hsp90 alpha with ab282108 at 1/50 (19.98 ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in 293T cells ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Western blot - Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)](https://www.abcam.com/ps/products/282/ab282108/Images/ab282108-5-anti-hsp90-antibody-68hsp90-western-blot-hela.jpg)
Western blot - Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)All lanes : Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate
Lane 2 : K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Observed band size: 83 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 7 seconds
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![Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)](https://www.abcam.com/ps/products/282/ab282108/Images/ab282108-2-anti-hsp90-antibody-68hsp90-immunocytochemistry-hela.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling Hsp90 alpha with ab282108 at 1/50 (19.98 ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in HeLa cells ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Anti-Hsp90 antibody [68/Hsp90] (ab282108)](https://www.abcam.com/ps/products/282/ab282108/Images/ab282108-3-Benefits-of-Recombinant-antibodies.jpg)
Anti-Hsp90 antibody [68/Hsp90] (ab282108)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab282108 has been referenced in 1 publication.
- Wei J et al. Cucurbitacin B-induced G2/M cell cycle arrest of conjunctival melanoma cells mediated by GRP78-FOXM1-KIF20A pathway. Acta Pharm Sin B 12:3861-3876 (2022). PubMed: 36213538