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    products/primary-antibodies/hsp90-alpha-antibody-68hsp90-ab282108.pdf

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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
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Validated using a knockout cell lineRecombinant

Recombinant Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)

  • Datasheet
  • SDS
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Western blot - Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)
  • Western blot - Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)
  • Anti-Hsp90 antibody [68/Hsp90] (ab282108)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [68/Hsp90] to Hsp90 alpha
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Hsp90 alpha antibody [68/Hsp90]
    See all Hsp90 alpha primary antibodies
  • Description

    Mouse monoclonal [68/Hsp90] to Hsp90 alpha
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type HEK-293T,Jurkat and HeLa, K-562 lysates. ICC: 293T, HeLa cells.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    68/Hsp90
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MMPs
    • Cancer
    • Tumor biomarkers
    • Other

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab282108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000.
ICC/IF
1/50.
Notes
WB
1/1000.
ICC/IF
1/50.
Application notes
Is unsuitable for IP.

Target

  • Function

    Molecular chaperone. Has ATPase activity.
  • Sequence similarities

    Belongs to the heat shock protein 90 family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P07900 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3320 Human
    • Omim: 140571 Human
    • SwissProt: P07900 Human
    • Unigene: 525600 Human
    • Unigene: 700831 Human
    • Alternative names

      • EL52 antibody
      • epididymis luminal secretory protein 52 antibody
      • Heat shock 86 kDa antibody
      • heat shock 90kD protein 1, alpha antibody
      • Heat shock 90kD protein 1, alpha like 4 antibody
      • heat shock 90kD protein, alpha-like 4 antibody
      • Heat shock 90kDa protein 1 alpha antibody
      • Heat shock protein 90kDa alpha (cytosolic) class A member 1 antibody
      • Heat shock protein HSP 90-alpha antibody
      • HS90A_HUMAN antibody
      • HSP 86 antibody
      • HSP86 antibody
      • Hsp89 antibody
      • HSP89A antibody
      • Hsp90 antibody
      • HSP90A antibody
      • HSP90AA1 antibody
      • HSP90ALPHA antibody
      • HSP90N antibody
      • HSPC1 antibody
      • HSPCA antibody
      • HSPCAL1 antibody
      • HSPCAL4 antibody
      • HSPN antibody
      • LAP 2 antibody
      • LAP2 antibody
      • lipopolysaccharide-associated protein 2 antibody
      • LPS-associated protein 2 antibody
      • Renal carcinoma antigen NY-REN-38 antibody
      see all

    Images

    • Western blot - Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)
      Western blot - Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)
      All lanes : Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108) at 1/1000 dilution

      Lane 1 : Wild-type HEK-293T (human embryonic kidney epithelial cell) whole cell lysate
      Lane 2 : HSP90AA1 KO HEK293T (human embryonic kidney epithelial cell) whole cell lysate
      Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG H&L (IRDye® 800CW) (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (ab216777) at 1/10000 dilution

      Observed band size: 83 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS.

      Lanes 1-3: Merged signal (red and green). Green - ab282108 observed at 83KDa. Red - loading control ab181602 (Rabbit monoclonal [EPR16891] to GAPDH) observed at 36 kDa.

      Lanes 1-2: ab282108 Anti-Hsp90 alpha antibody was shown to react with Hsp90 alpha in HEK293T cells in Western blot. Loss of signal was observed when HSP90AA1 knockout sample was used. Wild-type and
      HSP90AA1 knockout samples were subjected to SDS-PAGE.

      ab282108 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated at 4? overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680CW) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800RD) preadsorbed (ab216772) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)
      Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells labelling Hsp90 alpha with ab282108 at 1/50 (19.98 ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in 293T cells ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Western blot - Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)
      Western blot - Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)
      All lanes : Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108) at 1/1000 dilution

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate
      Lane 2 : K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

      Observed band size: 83 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Exposure time: 7 seconds

    • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)
      Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling Hsp90 alpha with ab282108 at 1/50 (19.98 ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in HeLa cells ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Anti-Hsp90 antibody [68/Hsp90] (ab282108)
      Anti-Hsp90 antibody [68/Hsp90] (ab282108)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab282108? Please let us know so that we can cite the reference in this datasheet.

    ab282108 has not yet been referenced specifically in any publications.

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