Recombinant Anti-Hsp90 alpha antibody [68/Hsp90] - BSA and Azide free (ab282128)
![Western blot - Anti-Hsp90 alpha antibody [68/Hsp90] - BSA and Azide free (ab282128)](https://www.abcam.com/ps/products/282/ab282128/Images/ab282128-4-anti-hsp90-antibody-68hsp90-western-blot-wildtype-hek293t.jpg "Western blot - Anti-Hsp90 alpha antibody [68/Hsp90] - BSA and Azide free (ab282128)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [68/Hsp90] to Hsp90 alpha - BSA and Azide free
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Hsp90 alpha antibody [68/Hsp90] - BSA and Azide free
See all Hsp90 alpha primary antibodies -
Description
Mouse monoclonal [68/Hsp90] to Hsp90 alpha - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HEK-293T,Jurkat and HeLa, K-562 lysates. ICC: 293T, HeLa cells.
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General notes
ab282128 is the carrier-free version of ab282108.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
68/Hsp90 -
Isotype
IgG1 -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab282128 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Molecular chaperone. Has ATPase activity. -
Sequence similarities
Belongs to the heat shock protein 90 family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 3320 Human
- Omim: 140571 Human
- SwissProt: P07900 Human
- Unigene: 525600 Human
- Unigene: 700831 Human
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Alternative names
- EL52 antibody
- epididymis luminal secretory protein 52 antibody
- Heat shock 86 kDa antibody
see all
Images
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Western blot - Anti-Hsp90 alpha antibody [68/Hsp90] - BSA and Azide free (ab282128)All lanes : Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : HSP90AA1 KO HEK293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (IRDye® 800CW) (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (ab216777) at 1/10000 dilution
Observed band size: 83 kDa why is the actual band size different from the predicted?This data was developed using ab282108, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS.
Lanes 1-3: Merged signal (red and green). Green - ab282108 observed at 83KDa. Red - loading control ab181602 (Rabbit monoclonal [EPR16891] to GAPDH) observed at 36 kDa.
Lanes 1-2: ab282108 Anti-Hsp90 alpha antibody was shown to react with Hsp90 alpha in HEK293T cells in Western blot. Loss of signal was observed when HSP90AA1 knockout sample was used. Wild-type and
HSP90AA1 knockout samples were subjected to SDS-PAGE.
ab282108 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated at 4? overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680CW) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800RD) preadsorbed (ab216772) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging. -
![Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 alpha antibody [68/Hsp90] - BSA and Azide free (ab282128)](https://www.abcam.com/ps/products/282/ab282128/Images/ab282128-1-anti-hsp90-antibody-68hsp90-immunocytochemistry-293t.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 alpha antibody [68/Hsp90] - BSA and Azide free (ab282128)This data was developed using ab282108 the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells labelling Hsp90 alpha with ab282108 at 1/50 (19.98 ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in 293T cells ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Western blot - Anti-Hsp90 alpha antibody [68/Hsp90] - BSA and Azide free (ab282128)](https://www.abcam.com/ps/products/282/ab282128/Images/ab282128-5-anti-hsp90-antibody-68hsp90-western-blot-hela.jpg)
Western blot - Anti-Hsp90 alpha antibody [68/Hsp90] - BSA and Azide free (ab282128)All lanes : Anti-Hsp90 alpha antibody [68/Hsp90] (ab282108) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate
Lane 2 : K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Observed band size: 83 kDa why is the actual band size different from the predicted?This data was developed using ab282108, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 7 seconds
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![Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 alpha antibody [68/Hsp90] - BSA and Azide free (ab282128)](https://www.abcam.com/ps/products/282/ab282128/Images/ab282128-2-anti-hsp90-antibody-68hsp90-immunocytochemistry-hela.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 alpha antibody [68/Hsp90] - BSA and Azide free (ab282128)This data was developed using ab282108 the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling Hsp90 alpha with ab282108 at 1/50 (19.98 ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in HeLa cells ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
-
![Anti-Hsp90 alpha antibody [68/Hsp90] - BSA and Azide free (ab282128)](https://www.abcam.com/ps/products/282/ab282128/Images/ab282128-3-Benefits-of-Recombinant-antibodies.jpg)
Anti-Hsp90 alpha antibody [68/Hsp90] - BSA and Azide free (ab282128)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab282128 has not yet been referenced specifically in any publications.
