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    products/primary-antibodies/hud--huc-antibody-epr19098-ab184267.pdf

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RecombinantRabMAb

Recombinant Anti-HuD + HuC antibody [EPR19098] (ab184267)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
  • Immunocytochemistry/ Immunofluorescence - Anti-HuD + HuC antibody [EPR19098] (ab184267)
  • Immunohistochemistry (Frozen sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
  • Western blot - Anti-HuD + HuC antibody [EPR19098] (ab184267)
  • Flow Cytometry (Intracellular) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
  • Immunoprecipitation - Anti-HuD + HuC antibody [EPR19098] (ab184267)
  • Immunocytochemistry/ Immunofluorescence - Anti-HuD + HuC antibody [EPR19098] (ab184267)
  • Western blot - Anti-HuD + HuC antibody [EPR19098] (ab184267)
  • Western blot - Anti-HuD + HuC antibody [EPR19098] (ab184267)
  • Western blot - Anti-HuD + HuC antibody [EPR19098] (ab184267)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
  • Immunohistochemistry (Frozen sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
  • Immunohistochemistry (Frozen sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
  • Immunohistochemistry (Frozen sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
  • Flow Cytometry (Intracellular) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
  • Anti-HuD + HuC antibody [EPR19098] (ab184267)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19098] to HuD + HuC
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, IHC-Fr, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-HuD + HuC antibody [EPR19098]
  • Description

    Rabbit monoclonal [EPR19098] to HuD + HuC
  • Host species

    Rabbit
  • Specificity

    This antibody shows very weak cross reactivity to HuB in WB test. Please contact our Scientific support team for more information.

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, IHC-Fr, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged mouse HuC recombinant protein; His-GST-tagged mouse HuD recombinant protein; Human fetal brain lysate; Mouse and rat brain lysates; Neuro-2a and SH-SY5Y cell lysates. IHC-P: Human cerebral cortex, colon and glioma tissues; Mouse cerebral cortex and stomach tissues; Rat cerebral cortex and colon tissues. IHC-Fr: Mouse cerebral cortex and stomach tissues; Rat cerebral cortex and colon tissues. ICC/IF: SH-SY5Y and Neuro-2a cells. Flow Cyt: SH-SY5Y and Neuro-2a cells. IP: Mouse brain lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19098
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Associated products

  • Alternative Versions

    • Anti-HuD + HuC antibody [EPR19098] - BSA and Azide free (ab232416)
    • Alexa Fluor® 488 Anti-HuD + HuC antibody [EPR19098] (ab237234)
    • Alexa Fluor® 647 Anti-HuD + HuC antibody [EPR19098] (ab237235)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/500.
WB
1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 40 kDa).
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/500.

Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).

ICC/IF
1/500.
IP
1/30.
Notes
Flow Cyt (Intra)
1/500.
WB
1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 40 kDa).
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/500.

Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).

ICC/IF
1/500.
IP
1/30.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)

    Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling HuC + HuD with ab184267 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Nuclear and weakly cytoplasmic staining on neurons of human cerebral cortex is observed [PMID: 22007133].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    Immunocytochemistry/ Immunofluorescence - Anti-HuD + HuC antibody [EPR19098] (ab184267)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling HuC + HuD with ab184267 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic and nuclear staining on SH-SY5Y cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    Immunohistochemistry (Frozen sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebral cortex tissue labeling HuC + HuD with ab184267 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Cytoplasmic and nuclear staining on neurons of mouse cerebral cortex is observed.

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Western blot - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    Western blot - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    All lanes : Anti-HuD + HuC antibody [EPR19098] (ab184267) at 1/2000 dilution

    Lane 1 : His-tagged mouse HuC recombinant protein (aa1-367)
    Lane 2 : His-GST-tagged mouse HuD recombinant protein (aa1-385)
    Lane 3 : His-tagged mouse HuR recombinant protein (aa1-360)
    Lane 4 : His-tagged mouse HuB recombinant protein (aa1-360)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 40 kDa
    Observed band size: 39,68 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 0.5 second.

    Please note that the band around 55kDa in lane 2 is most likely the HuD protein with tags.

  • Flow Cytometry (Intracellular) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    Flow Cytometry (Intracellular) - Anti-HuD + HuC antibody [EPR19098] (ab184267)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling HuC + HuDwith ab184267 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Immunoprecipitation - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    Immunoprecipitation - Anti-HuD + HuC antibody [EPR19098] (ab184267)

    HuC + HuD was immunoprecipitated from 0.35 mg of mouse brain lysate with ab184267 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab184267 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse brain lysate, 10 µg (Input).

    Lane 2: ab184267 IP in mouse brain lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184267 in mouse brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Immunocytochemistry/ Immunofluorescence - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    Immunocytochemistry/ Immunofluorescence - Anti-HuD + HuC antibody [EPR19098] (ab184267)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cell line) cells labeling HuC + HuD with ab184267 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic and nuclear staining on Neuro-2a cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Western blot - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    Western blot - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    All lanes : Anti-HuD + HuC antibody [EPR19098] (ab184267) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Predicted band size: 40 kDa
    Observed band size: 39 kDa why is the actual band size different from the predicted?


    Exposure time: 8 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile is consistent with what has been described in the literature (PMID: 9016658; PMID:8535975).

  • Western blot - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    Western blot - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    All lanes : Anti-HuD + HuC antibody [EPR19098] (ab184267) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Rat heart lysate
    Lane 6 : Rat kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 40 kDa
    Observed band size: 39 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-3: 1 minute; Lane 4-6: 5 seconds.

    The expression profile is consistent with what has been described in the literature (PMID: 9016658; PMID: 8535975).

  • Western blot - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    Western blot - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    All lanes : Anti-HuD + HuC antibody [EPR19098] (ab184267) at 1/5000 dilution

    Lane 1 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
    Lane 2 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 40 kDa
    Observed band size: 39 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution Buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling HuC + HuD with ab184267 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Nuclear and weakly cytoplasmic staining on myenteric ganglia of human colon is observed [PMID: 16918730].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)

    Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling HuC + HuD with ab184267 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Nuclear and weakly cytoplasmic staining on human glioma is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling HuC + HuD with ab184267 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Nuclear and weakly cytoplasmic staining on neurons of mouse cerebral cortex is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling HuC + HuD with ab184267 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic and nuclear staining on myenteric ganglia of mouse stomach is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling HuC + HuD with ab184267 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Nuclear and weakly cytoplasmic staining on neurons of rat cerebral cortex is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling HuC + HuD with ab184267 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic and nuclear staining on myenteric ganglia of rat colon is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    Immunohistochemistry (Frozen sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling HuC + HuD with ab184267 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Cytoplasmic and nuclear staining on myenteric ganglia of mouse stomach is observed (white arrow).

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    Immunohistochemistry (Frozen sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebral cortex tissue labeling HuC + HuD with ab184267 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Cytoplasmic and nuclear staining on neurons of rat cerebral cortex is observed.

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    Immunohistochemistry (Frozen sections) - Anti-HuD + HuC antibody [EPR19098] (ab184267)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat colon tissue labeling HuC + HuD with ab184267 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Cytoplasmic and nuclear staining on myenteric ganglia of rat colon is observed.

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-HuD + HuC antibody [EPR19098] (ab184267)
    Flow Cytometry (Intracellular) - Anti-HuD + HuC antibody [EPR19098] (ab184267)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Neuro-2a (Mouse neuroblastoma cell line) cells labeling HuC + HuDwith ab184267 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Anti-HuD + HuC antibody [EPR19098] (ab184267)
    Anti-HuD + HuC antibody [EPR19098] (ab184267)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (4)

Publishing research using ab184267? Please let us know so that we can cite the reference in this datasheet.

ab184267 has been referenced in 4 publications.

  • Yuan H  et al. Premigratory neural crest stem cells generate enteric neurons populating the mouse colon and regulating peristalsis in tissue-engineered intestine. Stem Cells Transl Med 10:922-938 (2021). PubMed: 33481357
  • Zeng Y  et al. The Impact of Particulate Matter (PM2.5) on Human Retinal Development in hESC-Derived Retinal Organoids. Front Cell Dev Biol 9:607341 (2021). PubMed: 33644046
  • Lai NY  et al. Gut-Innervating Nociceptor Neurons Regulate Peyer's Patch Microfold Cells and SFB Levels to Mediate Salmonella Host Defense. Cell 180:33-49.e22 (2020). PubMed: 31813624
  • Illing RB  et al. Mitotic activity, modulation of DNA processing, and purinergic signalling in the adult rat auditory brainstem following sensory deafferentation. Eur J Neurosci 50:3985-4003 (2019). PubMed: 31325398

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Immunocytochemistry abreview for Anti-HuD + HuC antibody [EPR19098]

Good
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Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cell (Human neurons)
Permeabilization
Yes - Triton X-100
Specification
Human neurons
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Nov 24 2021

IHC - Wholemount abreview for Anti-HuD + HuC antibody [EPR19098]

Excellent
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Rat Tissue (Jejunum myenteric plexus)
Specification
Jejunum myenteric plexus
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Nov 25 2019

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