Anti-HuD antibody [16C12AA10] (ab171448)
![Western blot - Anti-HuD antibody [16C12AA10] (ab171448)](https://www.abcam.com/ps/products/171/ab171448/Images/ab171448-172137-anti-hud-antibody-16c12aa10-western-blot.jpg "Western blot - Anti-HuD antibody [16C12AA10] (ab171448)")
Key features and details
- Mouse monoclonal [16C12AA10] to HuD
- Suitable for: WB, ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-HuD antibody [16C12AA10]
See all HuD primary antibodies -
Description
Mouse monoclonal [16C12AA10] to HuD -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Does not react with: Mouse, Rat, Cow -
Immunogen
Synthetic peptide corresponding to Human HuD aa 26-39 (N terminal) conjugated to keyhole limpet haemocyanin (Cysteine residue).
Sequence:SNNRNCPSPMQTGA
Database link: P26378 -
Positive control
- ELAVL4 recombinant protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
16C12AA10 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 2 µg/ml. Predicted molecular weight: 42 kDa.
|
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| ELISA |
Use a concentration of 1 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 42 kDa. |
|
ELISA
Use a concentration of 1 µg/ml. |
Target
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Function
May play a role in neuron-specific RNA processing. Protects CDKN1A mRNA from decay by binding to its 3'-UTR (By similarity). Binds to AU-rich sequences (AREs) of target mRNAs, including VEGF and FOS mRNA. -
Tissue specificity
Brain. -
Sequence similarities
Belongs to the RRM elav family.
Contains 3 RRM (RNA recognition motif) domains. -
Post-translational
modificationsMethylation at Arg-243 by CARM1 weakens protective binding to the 3'-UTR of CDKN1A mRNA and down-regulates CDKN1A protein expression, thereby maintaining cells in a proliferative state. Methylation is inhibited by NGF, which facilitates neurite outgrowth. - Information by UniProt
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Database links
- Entrez Gene: 1996 Human
- Omim: 168360 Human
- SwissProt: P26378 Human
- Unigene: 213050 Human
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Alternative names
- ELAV (embryonic lethal abnormal vision Drosophila) like 4 antibody
- ELAV L4 antibody
- ELAV like 4 antibody
see all
Images
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Western blot - Anti-HuD antibody [16C12AA10] (ab171448)Anti-HuD antibody [16C12AA10] (ab171448) at 2 µg/ml + Human ELAV4 recombinant protein at 0.100000001490116 µg
Secondary
Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution
Predicted band size: 42 kDa -
![ELISA - Anti-HuD antibody [16C12AA10] (ab171448)](https://www.abcam.com/ps/products/171/ab171448/Images/ab171448-172138-anti-hud-antibody-16c12aa10-elisa.jpg)
ELISA - Anti-HuD antibody [16C12AA10] (ab171448)Analysis demonstrating the specificity of ab171448 using the ELISA platform.
Antibody concentration is at 1 µg/mL, the concentration of recombinant proteins is at 250 ng/mL. The antibody reacts specifically to HuD/ELAV4 protein.
Datasheets and documents
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Datasheet download
References (0)
ab171448 has not yet been referenced specifically in any publications.
