Anti-Human Ig light chain antibody [MHK-49] (ab48536)
Key features and details
- Mouse monoclonal [MHK-49] to Human Ig light chain
- Suitable for: ICC/IF, Flow Cyt, ELISA
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Human Ig light chain antibody [MHK-49]
See all Human Ig light chain primary antibodies -
Description
Mouse monoclonal [MHK-49] to Human Ig light chain -
Host species
Mouse -
Specificity
This antibody is specific for both soluble and membrane human immunoglobulin light chain kappa. It does not react with human immunoglobulin light chain lambda or heavy chain mu. -
Tested applications
Suitable for: ICC/IF, Flow Cyt, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human Ig light chain. Human Ig cocktail
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MHK-49 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab48536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ELISA |
Use at an assay dependent concentration.
This antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis. |
| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ELISA
Use at an assay dependent concentration. This antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis. |
Target
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Relevance
There are only two types of light chain: kappa and lambda in mammals. Other types of light chains are found in lower vertebrates as the Ig-Light-Iota chain in Chondrichthyes and Teleostei. In each antibody, only one type is present and the two chains are identical. Each light chain has two successive domains: one constant and one variable domain. In humans 60% of light chains are kappa and 40% lambda,whereas in the mouse 95% of light chains are kappa. The amino acid sequences of lambda chains vary slightly at a few positions, allowing them to be classified into subtypes. The number of subtypes varies between species. Monoclonal immunoglobulin free light chains (FLC) are found in the serum and urine (Bence-Jones protein) of patients with a number of B-cell proliferative disorders, including multiple myeloma. Changes in serum FLC concentrations can provide a rapid and sensitive indication of response to treatment. -
Cellular localization
Cell Membrane: single-pass type I membrane protein and Secreted -
Database links
- Entrez Gene: 3514 Human
- Omim: 147200 Human
- SwissProt: P01834 Human
- SwissProt: Q502W4 Human
- SwissProt: Q569I9 Human
- SwissProt: Q6GMV9 Human
- SwissProt: Q6GMW1 Human
- SwissProt: Q6GMX8 Human
see all -
Alternative names
- HCAK1 antibody
- Ig kappa chain antibody
- IGKC antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab48536 has not yet been referenced specifically in any publications.