Anti-Human IgG4 heavy chain antibody [5C7] (ab1930)
Key features and details
- Mouse monoclonal [5C7] to Human IgG4 heavy chain
- Suitable for: ELISA
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Human IgG4 heavy chain antibody [5C7] -
Description
Mouse monoclonal [5C7] to Human IgG4 heavy chain -
Host species
Mouse -
Specificity
Subclass-specific for human IgG4. No allotype restriction. Fc-region specific. -
Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human Human IgG4 heavy chain.
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General notes
Concentration varies from lot to lot and can be provided on request.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Chromatography on protein A Sepharose -
Clonality
Monoclonal -
Clone number
5C7 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1930 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
Specific antibody titer is 1/100,000 by indirect ELISA.
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| Notes |
|---|
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ELISA
Use at an assay dependent concentration. Specific antibody titer is 1/100,000 by indirect ELISA.
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Target
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Relevance
Immunoglobulin G (IgG), is one of the most abundant protein in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B-lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 3503 Human
- Omim: 147130 Human
- SwissProt: P01861 Human
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Alternative names
- AU044919 antibody
- Gamma 2b immunoglobulin antibody
- Ig gamma 4 chain C region antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab1930 has been referenced in 3 publications.
- Yang F et al. Transcriptome Profiling Reveals B-Lineage Cells Contribute to the Poor Prognosis and Metastasis of Clear Cell Renal Cell Carcinoma. Front Oncol 11:731896 (2021). PubMed: 34485161
- Harlow DE et al. Myelin Proteolipid Protein Complexes with av Integrin and AMPA Receptors In Vivo and Regulates AMPA-Dependent Oligodendrocyte Progenitor Cell Migration through the Modulation of Cell-Surface GluR2 Expression. J Neurosci 35:12018-32 (2015). PubMed: 26311781
- Medeiros MM et al. Natural antibody response to Plasmodium falciparum merozoite antigens MSP5, MSP9 and EBA175 is associated to clinical protection in the Brazilian Amazon. BMC Infect Dis 13:608 (2013). ELISA ; Human . PubMed: 24373342