Anti-Human Nuclear Antigen antibody [235-1] (ab191181)
![Immunocytochemistry/ Immunofluorescence - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)](https://www.abcam.com/ps/products/191/ab191181/Images/ab191181-11-anti-nuclear-antigen-antibody-235-1-immunocytochemistry-mcf7-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)")
Key features and details
- Mouse monoclonal [235-1] to Human Nuclear Antigen
- Suitable for: Flow Cyt, ICC/IF, IHC-Fr
- Reacts with: Human, Non human primates
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-Human Nuclear Antigen antibody [235-1]
See all Human Nuclear Antigen primary antibodies -
Description
Mouse monoclonal [235-1] to Human Nuclear Antigen -
Host species
Mouse -
Specificity
ab191181 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in frozen tissue sections. It can be used with paraformaldehyde-fixed frozen tissue or cell preparations. -
Tested applications
Suitable for: Flow Cyt, ICC/IF, IHC-Frmore details -
Species reactivity
Reacts with: Human, Non human primates
Does not react with: Mouse, Rat, Chicken -
Immunogen
Tissue, cells or virus corresponding to Human Human Nuclear Antigen. Nuclei of Human myeloid leukemia biopsy cells
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Positive control
- IHC-Fr: Human tonsil tissue; ICC/IF: MCF and K562 cells; Flow Cyt: Raji, MCF7, K562, HeLa, and Jurkat cells.
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General notes
ab190710 is recommended for IHC-P with paraffin embedded sections.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
235-1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | (1) |
Use a concentration of 1 - 2 µg/ml.
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| IHC-Fr |
Use a concentration of 0.5 - 1 µg/ml.
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| Notes |
|---|
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Flow Cyt
Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF
Use a concentration of 1 - 2 µg/ml. |
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IHC-Fr
Use a concentration of 0.5 - 1 µg/ml. |
Target
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Alternative names
- HNA antibody
- HuNu antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)PFA-fixed MCF-7 (human breast adenocarcinoma cell line) cells stained for Human Nuclear Antigen using ab191181 at 2 ug/ml in ICC.
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![Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)](https://www.abcam.com/ps/products/191/ab191181/Images/ab191181-9-anti-nuclear-antigen-antibody-235-1-flow-cytometry-raji-human.jpg)
Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)Flow Cytometric analysis of paraformaldehyde-fixed Raji (human Burkitt's lymphoma cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red). -
![Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)](https://www.abcam.com/ps/products/191/ab191181/Images/ab191181-336808-anti-human-nuclear-antigen-antibody-235-1-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)Rockne et al PLoS One. 2018 Aug 2;13(8):e0199967. doi: 10.1371/journal.pone.0199967. eCollection 2018. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Characterization of LM-NSC008 cells.
Visualization of LM-NSC008 cells in naïve non-tumor bearing mouse brain 6 and 9 months post administration. (A) Immunohistochemistry (IHC) staining of LM-NSC008 cells (stably expressing eGFP/Ffluc) with anti-HNA antibodies (NSC injection site, white arrows indicate the LM-NSC008 cells, scale bar 100 μm). (1a-1d) Insets show LM-NSC008 cells co-expressing HNA and eGFP proteins (inset scale bars [1a-1f] 2 μm).
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![Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)](https://www.abcam.com/ps/products/191/ab191181/Images/ab191181-280636-anti-human-nuclear-antigen-antibody-235-1-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)Cold acetone-fixed frozen human tonsil tissue stained for Human Nuclear Antigen with ab191181 at 0.5 µg/ml (30 mins, RT) in immunohistochemical analysis. Visualised using a HRP-Polymer detection system.
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![Immunocytochemistry/ Immunofluorescence - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)](https://www.abcam.com/ps/products/191/ab191181/Images/ab191181-10-anti-nuclear-antigen-antibody-235-1-immunocytochemistry-k562-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow ) cells stained for Human Nuclear Antigen using ab191181 at 2 ug/ml in ICC. -
![Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)](https://www.abcam.com/ps/products/191/ab191181/Images/ab191181-8-anti-nuclear-antigen-antibody-235-1-flow-cytometry-mcf7-human.jpg)
Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)Flow Cytometric analysis of paraformaldehyde-fixed MCF-7 (human breast adenocarcinoma cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red). -
![Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)](https://www.abcam.com/ps/products/191/ab191181/Images/ab191181-7-anti-nuclear-antigen-antibody-235-1-flow-cytometry-k562-human.jpg)
Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)Flow Cytometric analysis of paraformaldehyde-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow ) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red). -
![Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)](https://www.abcam.com/ps/products/191/ab191181/Images/ab191181-6-anti-nuclear-antigen-antibody-235-1-flow-cytometry-jurkat-human.jpg)
Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)Flow Cytometric analysis of paraformaldehyde-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red). -
![Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)](https://www.abcam.com/ps/products/191/ab191181/Images/ab191181-5-anti-nuclear-antigen-antibody-235-1-flow-cytometry-hela-human.jpg)
Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (ab191181)Flow Cytometric analysis of paraformaldehyde-fixed HeLa (human lymphoblastic leukemia cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (43)
ab191181 has been referenced in 43 publications.
- Zhao Y et al. Human ESC-derived immunity- and matrix- regulatory cells ameliorated white matter damage and vascular cognitive impairment in rats subjected to chronic cerebral hypoperfusion. Cell Prolif 55:e13223 (2022). PubMed: 35437845
- Ikeda M et al. TERT/BMI1-transgenic human dermal papilla cells enhance murine hair follicle formation in vivo. J Dermatol Sci 106:78-85 (2022). PubMed: 35440400
- Chao J et al. Therapeutic development for Canavan disease using patient iPSCs introduced with the wild-type ASPA gene. iScience 25:104391 (2022). PubMed: 35637731
- Zhao P et al. LILRB2-mediated TREM2 signaling inhibition suppresses microglia functions. Mol Neurodegener 17:44 (2022). PubMed: 35717259
- Gonzaga VF et al. Therapeutic Potential of Human Immature Dental Pulp Stem Cells Observed in Mouse Model for Acquired Aplastic Anemia. Cells 11:N/A (2022). PubMed: 35883695