Anti-Hunchback antibody [F18-1G10.2] (ab197787)
![Immunocytochemistry/ Immunofluorescence - Anti-Hunchback antibody [F18-1G10.2] (ab197787)](https://www.abcam.com/ps/products/197/ab197787/Images/ab197787-238019-anti-hunchback-antibody-f18-1g102-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Hunchback antibody [F18-1G10.2] (ab197787)")
Key features and details
- Mouse monoclonal [F18-1G10.2] to Hunchback
- Suitable for: ICC/IF
- Reacts with: Drosophila melanogaster
- Isotype: IgG1
Overview
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Product name
Anti-Hunchback antibody [F18-1G10.2] -
Description
Mouse monoclonal [F18-1G10.2] to Hunchback -
Host species
Mouse -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Drosophila melanogaster -
Immunogen
The details of the immunogen for this antibody are not available.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Purity
SDS-PAGE -
Clonality
Monoclonal -
Clone number
F18-1G10.2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Gap class segmentation protein that controls development of head structures. -
Tissue specificity
In embryo, expression of maternal transcript is highest in anterior region. Zygotic transcript is expressed in anterior region until the beginning of gastrulation and in posterior region until early gastrulation. After this, it is expressed in developing nervous system. -
Sequence similarities
Belongs to the hunchback C2H2-type zinc-finger protein family.
Contains 6 C2H2-type zinc fingers. -
Developmental stage
Expressed maternally and zygotically. Expression of the maternal transcript decreases until embryonic stage 14, zygotic transcript is first detected at stage 11. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 41032 Drosophila melanogaster
- SwissProt: P05084 Drosophila melanogaster
- Unigene: 1105 Drosophila melanogaster
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Alternative names
- CG9786 antibody
- Dmel\CG9786 antibody
- hb antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Hunchback antibody [F18-1G10.2] (ab197787)
Immunocytochemistry analysis using Hunchback mouse monoclonal F18-1G10 to detect Hunchback protein in all neuroblast nuclei from Drosophila stage 9 embryos. The cells were fixed in 4% formaldehyde for 25 minutes, permeabilized with 0.1% Triton X-100/PBS, and blocked in 0.1% Triton X-100/PBS, 2.5% Normal Goat Serum, 2.5% Normal Donkey Serum. The Hunchback stain used mouse monoclonal F18-1G10 at 10 µg/mL and was detected with Alexa555 conjugated goat anti-mouse (at 10 µg/mL).
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab197787 has been referenced in 1 publication.
- Yang Z et al. The dynamic transmission of positional information in stau- mutants during Drosophila embryogenesis. Elife 9:N/A (2020). PubMed: 32511091
