Anti-Huntingtin antibody (ab155942)
Key features and details
- Rabbit polyclonal to Huntingtin
- Suitable for: IHC, WB, ELISA, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-Huntingtin antibody
See all Huntingtin primary antibodies -
Description
Rabbit polyclonal to Huntingtin -
Host species
Rabbit -
Specificity
ab155942 recognizes the 552 cleaved fragment without detecting the full-length form. -
Tested applications
Suitable for: IHC, WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Huntingtin aa 544-552 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue).
Sequence:SDPAMDLND
Database link: P42858 -
Positive control
- Huntingtin protein and protein fragments; 293T cells; Mouse cortex tissue; Endogenous Huntingtin lysates, with or without caspase activity.
-
General notes
Human HTT caspase cleavage sites generate fragment-specific forms of the protein. Caspase-3/7 has been shown to generate cleavage sites at animo acids 513 and 552. Caspase-2 cleaves at amino acid 552 and caspase-6 at amino acid 586.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
Human HTT caspase cleavage sites generate fragment-specific forms of the protein. Caspase-3/7 has been shown to generate cleavage sites at animo acids 513 and 552. Caspase-2 cleaves at amino acid 552 and caspase-6 at amino acid 586. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC |
1/50 - 1/200.
|
|
| WB |
1/500. Predicted molecular weight: 348 kDa.
|
|
| ELISA |
1/20 - 1/100.
|
|
| ICC/IF |
1/50 - 1/200.
|
| Notes |
|---|
|
IHC
1/50 - 1/200. |
|
WB
1/500. Predicted molecular weight: 348 kDa. |
|
ELISA
1/20 - 1/100. |
|
ICC/IF
1/50 - 1/200. |
Target
-
Function
May play a role in microtubule-mediated transport or vesicle function. -
Tissue specificity
Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation. -
Involvement in disease
Defects in HTT are the cause of Huntington disease (HD) [MIM:143100]. HD is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Onset of the disease occurs usually in the third or fourth decade of life and symptoms progressively worsen leading to death in 10 to 20 years. Onset and clinical course depend on the degree of poly-Gln repeat expansion, longer expansions resulting in earlier onset and more severe clinical manifestations. HD affects 1 in 10,000 individuals of European origin. Neuropathology of Huntington disease displays a distinctive pattern with loss of neurons, especially in the caudate and putamen (striatum). -
Sequence similarities
Belongs to the huntingtin family.
Contains 10 HEAT repeats. -
Domain
The N-terminal Gln-rich and Pro-rich domain has great conformational flexibility and is likely to exist in a fluctuating equilibrium of alpha-helical, random coil, and extended conformations. -
Post-translational
modificationsCleaved by apopain downstream of the polyglutamine stretch. The resulting N-terminal fragment is cytotoxic and provokes apoptosis.
Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus. The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington's disease neurons. - Information by UniProt
-
Database links
- Entrez Gene: 3064 Human
- Entrez Gene: 15194 Mouse
- Omim: 143100 Human
- Omim: 613004 Human
- SwissProt: P42858 Human
- SwissProt: P42859 Mouse
- Unigene: 518450 Human
- Unigene: 209071 Mouse
see all -
Alternative names
- AI256365 antibody
- C430023I11Rik antibody
- HD antibody
see all
Images
-
Western blot - Anti-Huntingtin antibody (ab155942)All lanes : Anti-Huntingtin antibody (ab155942) at 1/500 dilution
Lane 1 : -
Lane 2 : +C2
Lane 3 : +C3
Lane 4 : +C6
Lane 5 : +C7
Lane 6 : Huntingtin23Q(1-513)
Lane 7 : Huntingtin23Q(1-536)
Lane 8 : Huntingtin23Q(1-552)
Lane 9 : Huntingtin23Q(1-586)
Lysates/proteins at 20 µg per lane.
Predicted band size: 348 kDa
Additional bands at: 62 kDa (possible cleavage fragment)
Lanes 1-5 are 293Tcells expressing the full length Htt protein and treated with the various caspases. Lanes 6-9 are 293T overexpressing the different Htt fragments to demonstrate the antibody’s specificity to that neo-epitope. Probed with N-terminal pan-Huntingtin antibody or (bottom panel) neoepitope specific rabbit polyclonal antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-Huntingtin antibody (ab155942)Immunofluorescent analysis of 293T cells (transfected with Huntingtin23Q and Huntingtin148Q stop constructs ending in amino acid 552), labeling Huntingtin ab155942 at 1/50 dilution. -
Immunohistochemistry - Anti-Huntingtin antibody (ab155942)Immunohistochemistry analysis of Mouse cortex tissue samples, labeling Huntingtin with ab155942 at 1/50 dilution (green). Blue: Tissue counterstained with nuclei stain. -
ELISA - Anti-Huntingtin antibody (ab155942)Sandwich Elisa analysis of 293T cells (overexpressing Huntingtin neo epitope 552), labeling Huntingtin protein (0, 0.5,1,1.5 and 2µg) with ab155942 at 1/20 dilution.
Protocols
Datasheets and documents
-
Datasheet download
References (0)
ab155942 has not yet been referenced specifically in any publications.
