Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193)
Key features and details
- Mouse monoclonal [19F12AE12] to HuR / ELAVL1
- Suitable for: WB, ICC/IF, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-HuR / ELAVL1 antibody [19F12AE12]
See all HuR / ELAVL1 primary antibodies -
Description
Mouse monoclonal [19F12AE12] to HuR / ELAVL1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Does not react with: Cow -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SW480, HEK293, HCT116, HepG2, HeLa, SH-SY5Y, Jurkat, Rat H4IIE, Mouse MEF, Mouse 3T3 and Rat H9C2 cell lysates; Human liver and heart homogenates. ICC/IF: SH-SY5Y and HeLa cells. Flow Cyt: HeLa cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading...
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Purity
Ammonium Sulphate Precipitation -
Clonality
Monoclonal -
Clone number
19F12AE12 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa.
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ICC/IF |
Use a concentration of 5 µg/ml.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. |
ICC/IF
Use a concentration of 5 µg/ml. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the RRM elav family.
Contains 3 RRM (RNA recognition motif) domains. -
Post-translational
modificationsMethylated at Arg-217 by CARM1 in macrophages in response to LPS challenge. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1994 Human
- Entrez Gene: 15568 Mouse
- Entrez Gene: 363854 Rat
- Omim: 603466 Human
- SwissProt: Q15717 Human
- SwissProt: P70372 Mouse
- Unigene: 184492 Human
- Unigene: 713744 Human
see all -
Alternative names
- ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody
- ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody
- ELAV like 1 antibody
see all
Images
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All lanes : Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193) at 2 µg/ml
Lane 1 : Wild-type SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate at 40 µg
Lane 2 : ELAVL1 knockout SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate at 40 µg
Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 4 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDaLanes 1 - 4: Merged signal (red and green). Green - ab170193 observed at 36 kDa. Red - loading control, ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.
ab170193 was shown to react with ELAVL1 in wild-type SW480 cells in western blot. Loss of signal was observed when ELAVL1 knockout sample was used. Wild-type and ELAVL1 knockout SW480 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBS-T (0.1% Tween®) before incubation with ab170193 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 2 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunocytochemistry on HeLa cells.
Cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15minutes). The cells were then incubated with the anti-HuR antibody (5µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody used was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. A cytoplasmic antibody (ab75834) was used as counter stain (green). 10% goat serum was used as a blocking agent for all blocking steps.
The target protein localizes mainly to the nucleus.
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All lanes : Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193) at 1 µg/ml
Lane 1 : Human Liver Homogenate
Lane 2 : Human Heart Homogenate
Lane 3 : HepG2 Cell Lysate
Lane 4 : HeLa Cell Lysate
Lane 5 : SH-SY5Y Cell Lysate
Lane 6 : Jurkat Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-mouse IR800 at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa -
All lanes : Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193) at 1 µg/ml
Lane 1 : H9C2 Rat Cell Lysate
Lane 2 : H4IIE Rat Cell Lysate
Lane 3 : MEF Mouse Cell Lysate
Lane 4 : 3T3 Mouse Cell Lysate
Lane 5 : SH-SY5Y Cell Lysate
Lane 6 : HepG2 Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-mouse AP at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa -
Immunocytochemistry on SH-SY5Y cells (Human neuroblastoma cell line).
Cells cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were then incubated with the anti-HuR antibody (5µg/mL) for 2 hours at room temperature or overnight at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. A cytoplasmic antibody (ab75834) was used as counter stain (Green). 10% Goat serum was used as a blocking agent for all blocking steps.
The target protein localizes mainly to the nucleus.
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Flow cytometric analysis of methanol-fixed HeLa cells labeling HuR / ELAVL1 with ab170193 at 1µg/mL (blue) or an equal amount of an isotype control antibody (red).
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab170193 has been referenced in 1 publication.
- Rothamel K et al. ELAVL1 primarily couples mRNA stability with the 3' UTRs of interferon-stimulated genes. Cell Rep 35:109178 (2021). PubMed: 34038724