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  1. Link

    products/primary-antibodies/hur--elavl1-antibody-19f12ae12-ab170193.pdf

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Validated using a knockout cell line

Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193)

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Western blot - Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193)
  • Immunocytochemistry/ Immunofluorescence - Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193)
  • Western blot - Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193)
  • Western blot - Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193)
  • Immunocytochemistry/ Immunofluorescence - Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193)
  • Flow Cytometry - Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193)

Key features and details

  • Mouse monoclonal [19F12AE12] to HuR / ELAVL1
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

You may also be interested in

Protein
Product image
Recombinant Human HuR / ELAVL1 protein (ab152360)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Knockout
Product image
Human ELAVL1 knockout SW480 cell line (ab270485)

View more associated products

Overview

  • Product name

    Anti-HuR / ELAVL1 antibody [19F12AE12]
    See all HuR / ELAVL1 primary antibodies
  • Description

    Mouse monoclonal [19F12AE12] to HuR / ELAVL1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Does not react with: Cow
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SW480, HEK293, HCT116, HepG2, HeLa, SH-SY5Y, Jurkat, Rat H4IIE, Mouse MEF, Mouse 3T3 and Rat H9C2 cell lysates; Human liver and heart homogenates. ICC/IF: SH-SY5Y and HeLa cells. Flow Cyt: HeLa cells.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.5
    Preservative: 0.02% Sodium azide
    Constituent: 99% HEPES buffered saline
  • Concentration information loading...
  • Purity

    Ammonium Sulphate Precipitation
  • Clonality

    Monoclonal
  • Clone number

    19F12AE12
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • RNAi
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Argonaute and Piwi

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • KO cell lines

    • Human ELAVL1 knockout SW480 cell line (ab270485)
  • KO cell lysates

    • Human ELAVL1 knockout SW480 cell lysate (ab270508)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human HuR / ELAVL1 protein (ab152360)
  • Related Products

    • Recombinant Human HuR / ELAVL1 protein (ab152360)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab170193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa.
ICC/IF
Use a concentration of 5 µg/ml.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 

Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa.
ICC/IF
Use a concentration of 5 µg/ml.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 

Target

  • Function

    Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the RRM elav family.
    Contains 3 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q15717 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1994 Human
    • Entrez Gene: 15568 Mouse
    • Entrez Gene: 363854 Rat
    • Omim: 603466 Human
    • SwissProt: Q15717 Human
    • SwissProt: P70372 Mouse
    • Unigene: 184492 Human
    • Unigene: 713744 Human
    • Unigene: 119162 Mouse
    see all
  • Alternative names

    • ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody
    • ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody
    • ELAV like 1 antibody
    • ELAV like RNA binding protein 1 antibody
    • ELAV-like protein 1 antibody
    • ELAV1 antibody
    • ELAV1_HUMAN antibody
    • Elavl1 antibody
    • Embryonic lethal abnormal vision drosophila homolog like 1 antibody
    • Hu Antigen R antibody
    • Hu-antigen R antibody
    • Hua antibody
    • HuR antibody
    • MelG antibody
    see all

Images

  • Western blot - Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193)
    Western blot - Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193)
    All lanes : Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193) at 2 µg/ml

    Lane 1 : Wild-type SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate at 40 µg
    Lane 2 : ELAVL1 knockout SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate at 40 µg
    Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
    Lane 4 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab170193 observed at 36 kDa. Red - loading control, ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.

    ab170193 was shown to react with ELAVL1 in wild-type SW480 cells in western blot. Loss of signal was observed when ELAVL1 knockout sample was used. Wild-type and ELAVL1 knockout SW480 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBS-T (0.1% Tween®) before incubation with ab170193 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 2 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193)
    Immunocytochemistry/ Immunofluorescence - Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193)

    Immunocytochemistry on HeLa cells.

    Cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15minutes). The cells were then incubated with the anti-HuR antibody (5µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody used was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. A cytoplasmic antibody (ab75834) was used as counter stain (green). 10% goat serum was used as a blocking agent for all blocking steps.

    The target protein localizes mainly to the nucleus.

  • Western blot - Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193)
    Western blot - Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193)
    All lanes : Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193) at 1 µg/ml

    Lane 1 : Human Liver Homogenate
    Lane 2 : Human Heart Homogenate
    Lane 3 : HepG2 Cell Lysate
    Lane 4 : HeLa Cell Lysate
    Lane 5 : SH-SY5Y Cell Lysate
    Lane 6 : Jurkat Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-mouse IR800 at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 36 kDa

  • Western blot - Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193)
    Western blot - Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193)
    All lanes : Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193) at 1 µg/ml

    Lane 1 : H9C2 Rat Cell Lysate
    Lane 2 : H4IIE Rat Cell Lysate
    Lane 3 : MEF Mouse Cell Lysate
    Lane 4 : 3T3 Mouse Cell Lysate
    Lane 5 : SH-SY5Y Cell Lysate
    Lane 6 : HepG2 Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-mouse AP at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 36 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193)
    Immunocytochemistry/ Immunofluorescence - Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193)

    Immunocytochemistry on SH-SY5Y cells (Human neuroblastoma cell line).

    Cells cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were then incubated with the anti-HuR antibody (5µg/mL) for 2 hours at room temperature or overnight at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. A cytoplasmic antibody (ab75834) was used as counter stain (Green). 10% Goat serum was used as a blocking agent for all blocking steps.

    The target protein localizes mainly to the nucleus.  

  • Flow Cytometry - Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193)
    Flow Cytometry - Anti-HuR / ELAVL1 antibody [19F12AE12] (ab170193)

    Flow cytometric analysis of methanol-fixed HeLa cells labeling HuR / ELAVL1 with ab170193 at 1µg/mL (blue) or an equal amount of an isotype control antibody (red).

Protocols

  • Flow cytometry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (0)

Publishing research using ab170193? Please let us know so that we can cite the reference in this datasheet.

ab170193 has not yet been referenced specifically in any publications.

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