Anti-HuR / ELAVL1 antibody [4C8] (ab136542)
![Western blot - Anti-HuR / ELAVL1 antibody [4C8] (ab136542)](https://www.abcam.com/ps/products/136/ab136542/Images/ab136542-397384-anti-hur-elavl1-antibody-4C8-western-blot-sw480-wt-sw480-elavl1-knockout-hek293-hct116.jpg "Western blot - Anti-HuR / ELAVL1 antibody [4C8] (ab136542)")
Key features and details
- Mouse monoclonal [4C8] to HuR / ELAVL1
- Suitable for: Flow Cyt, WB, EMSA
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-HuR / ELAVL1 antibody [4C8]
See all HuR / ELAVL1 primary antibodies -
Description
Mouse monoclonal [4C8] to HuR / ELAVL1 -
Host species
Mouse -
Specificity
ab136542 was specifically selected because of it's abiity to supershift HuR/mRNA ribonucleoprotein complexes. ab136542 does not react with HuD, HuC, or Hel-N1. -
Tested applications
Suitable for: Flow Cyt, WB, EMSAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to a region within HuR/ ELAVL1.
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Positive control
- WB: SW480, HEK293 and HCT116 cell lysates. Flow Cyt: MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
4C8 -
Isotype
IgG1 -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab136542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
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| EMSA |
Use at an assay dependent concentration.
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| Notes |
|---|
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa. |
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EMSA
Use at an assay dependent concentration. |
Target
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Function
Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the RRM elav family.
Contains 3 RRM (RNA recognition motif) domains. -
Post-translational
modificationsMethylated at Arg-217 by CARM1 in macrophages in response to LPS challenge. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1994 Human
- Omim: 603466 Human
- SwissProt: Q15717 Human
- Unigene: 184492 Human
- Unigene: 713744 Human
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Alternative names
- ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody
- ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody
- ELAV like 1 antibody
see all
Images
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Western blot - Anti-HuR / ELAVL1 antibody [4C8] (ab136542)All lanes : Anti-HuR / ELAVL1 antibody [4C8] (ab136542) at 1 µg/ml
Lane 1 : Wild-type SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate at 40 µg
Lane 2 : ELAVL1 knockout SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate at 40 µg
Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 4 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDaLanes 1 - 4: Merged signal (red and green). Green - ab136542 observed at 36 kDa. Red - loading control, ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.
ab136542 was shown to react with ELAVL1 in wild-type SW480 cells in western blot. Loss of signal was observed when ELAVL1 knockout sample was used. Wild-type and ELAVL1 knockout SW480 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBS-T (0.1% Tween®) before incubation with ab136542 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry - Anti-HuR / ELAVL1 antibody [4C8] (ab136542)](https://www.abcam.com/ps/products/136/ab136542/Images/ab136542-204489-anti-hur-elavl1-antibody-4c8-flow-cytometry.jpg)
Flow Cytometry - Anti-HuR / ELAVL1 antibody [4C8] (ab136542)Overlay histogram showing MCF7 cells stained with ab136542 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab136542, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![Electrophoretic Mobility Shift Assay - Anti-HuR / ELAVL1 antibody [4C8] (ab136542)](https://www.abcam.com/ps/products/136/ab136542/Images/ab136542-151164-anti-hur-elavl1-antibody-4c8-electrophoretic-mobility-shift-assay.jpg)
Electrophoretic Mobility Shift Assay - Anti-HuR / ELAVL1 antibody [4C8] (ab136542)Electrophoretic Mobility Shift Assay of HuR/mRNA complex (Lane 1). The complex is significanlty supershifted by the addition of 10ng of ab136542 (Lane 2).
Datasheets and documents
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Datasheet download
References (12)
ab136542 has been referenced in 12 publications.
- Han X et al. Loss of RNA-Binding Protein HuR Leads to Defective Ependymal Cells and Hydrocephalus. J Neurosci 42:202-219 (2022). PubMed: 34815315
- Maniaci M et al. Systematic Analysis of the Impact of R-Methylation on RBPs-RNA Interactions: A Proteomic Approach. Front Mol Biosci 8:688973 (2021). PubMed: 34557518
- Vilmundarson RO et al. IRF2BP2 3'UTR Polymorphism Increases Coronary Artery Calcification in Men. Front Cardiovasc Med 8:687645 (2021). PubMed: 34760935
- Li K et al. Stabilization of oncogenic transcripts by the IGF2BP3/ELAVL1 complex promotes tumorigenicity in colorectal cancer. Am J Cancer Res 10:2480-2494 (2020). PubMed: 32905413
- Chen R et al. LncRNA HOXB-AS1 promotes cell growth in multiple myeloma via FUT4 mRNA stability by ELAVL1. J Cell Biochem 121:4043-4051 (2020). PubMed: 31886581
