Recombinant Anti-HuR / ELAVL1 antibody [EPR17397] KO Tested (ab200342)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

Loading size & price…

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17397] to HuR / ELAVL1
Suitable for: IHC-P, WB, ICC/IF, IP, Flow Cyt (Intra)
Knockout validated
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free PE

Product name

Anti-HuR / ELAVL1 antibody [EPR17397]
See all HuR / ELAVL1 primary antibodies
Description

Rabbit monoclonal [EPR17397] to HuR / ELAVL1
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WB, ICC/IF, IP, Flow Cyt (Intra)more details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: SW480, HAP1, 293, HeLa, Jurkat and K562 whole cell lysates; Human fetal brain, fetal kidney and fetal heart lysates; Mouse heart, mouse kidney, rat brain and rat spleen lysates. IHC-P: Human cervix carcinoma, mouse cardiac muscle and rat cerebral cortex tissues. ICC/IF: HeLa cells. IP: HeLa whole cell lysate. Flow: Jurkat (human acute T cell leukemia)
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17397
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human ELAVL1 knockout SW480 cell line (ab270485)
KO cell lysates
- Human ELAVL1 knockout SW480 cell lysate (ab270508)
Recombinant Protein
- Recombinant Human HuR / ELAVL1 protein (ab152360)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab200342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
ICC/IF		1/250.
IP		1/30.
Flow Cyt (Intra)		Use a concentration of 0.1 µg/ml.

Notes
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
ICC/IF 1/250.
IP 1/30.
Flow Cyt (Intra) Use a concentration of 0.1 µg/ml.

Function

Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
Tissue specificity

Ubiquitous.
Sequence similarities

Belongs to the RRM elav family.
Contains 3 RRM (RNA recognition motif) domains.
Post-translational
modifications

Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
Cellular localization

Cytoplasm.
Target information above from: UniProt accession Q15717 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 1994 Human
- Entrez Gene: 15568 Mouse
- Entrez Gene: 363854 Rat
- Omim: 603466 Human
- SwissProt: Q15717 Human
- SwissProt: P70372 Mouse
- Unigene: 184492 Human
- Unigene: 713744 Human
- Unigene: 119162 Mouse
see all
Alternative names
- ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody
- ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody
- ELAV like 1 antibody
- ELAV like RNA binding protein 1 antibody
- ELAV-like protein 1 antibody
- ELAV1 antibody
- ELAV1_HUMAN antibody
- Elavl1 antibody
- Embryonic lethal abnormal vision drosophila homolog like 1 antibody
- Hu Antigen R antibody
- Hu-antigen R antibody
- Hua antibody
- HuR antibody
- MelG antibody
see all

Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)

All lanes : Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) at 1/5000 dilution

Lane 1 : Wild-type SW480 (Human colorectal adenocarcinoma cell line) cell lysate
Lane 2 : ELAVL1 knockout SW480 (Human colorectal adenocarcinoma cell line) cell lysate

Lysates/proteins at 40 µg per lane.

Performed under reducing conditions.

Predicted band size: 36 kDa
Observed band size: 36 kDa

Lanes 1 - 2: Merged signal (red and green). Green - ab200342 observed at 36 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

ab200342 was shown to react with ELAVL1 in wild-type SW480 cells in western blot with loss of signal observed in ELAVL1 knockout sample. Wild-type and ELAVL1 knockout SW480 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab200342 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Flow Cytometry (Intracellular) - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)

ab200342 staining HuR / ELAVL1 in Jurkat (human acute T cell leukemia) cellsby intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/23000. A goat anti rabbit IgG (Alexa Fluor^® 488) at a dilution of 1/2000 was used as the secondary antibody.

Isoytype control: Rabbit monoclonal IgG (Black)

Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)

All lanes : Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) at 1/5000 dilution

Lane 1 : 293 (Human embryonic kidney) whole cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 4 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time: 5 seconds

Blocking/dilution buffer: 5% NFDM/TBST.
Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)

All lanes : Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time: 5 seconds

Blocking/dilution buffer: 5% NFDM/TBST.
Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)

Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) at 1/1000 dilution + Human fetal heart lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time: 1 minute

Blocking/dilution buffer: 5% NFDM/TBST.
Western blot - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)

All lanes : Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) at 1/1000 dilution

Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time: 5 seconds

Blocking/dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)

Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling HuR / ELAVL1 with ab200342 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling HuR / ELAVL1 with ab200342 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on Mouse cardiac muscle tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling HuR / ELAVL1 with ab200342 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on rat cerebral cortex tissue tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling HuR / ELAVL1 with ab200342 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI.
Immunoprecipitation - Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)

HuR / ELAVL1 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200342 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab200342 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HeLa whole cell lysate 10 µg (Input). Lane 2: ab200342 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200342 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab200342? Please let us know so that we can cite the reference in this datasheet.

ab200342 has been referenced in 45 publications.

Zheng R et al. Fine Particulate Matter Induces Childhood Asthma Attacks via Extracellular Vesicle-Packaged Let-7i-5p-Mediated Modulation of the MAPK Signaling Pathway. Adv Sci (Weinh) 9:e2102460 (2022). PubMed: 34816611
Cui YR et al. KPT330 improves Cas9 precision genome- and base-editing by selectively regulating mRNA nuclear export. Commun Biol 5:237 (2022). PubMed: 35301428
Zhou HJ et al. lncRNA MEG3 restrained the M1 polarization of microglia in acute spinal cord injury through the HuR/A20/NF-κB axis. Brain Pathol 32:e13070 (2022). PubMed: 35338543
Chen G et al. Circular RNA circStag1 promotes bone regeneration by interacting with HuR. Bone Res 10:32 (2022). PubMed: 35361779
Du Y et al. SOCS7/HuR/FOXM1 signaling axis inhibited high-grade serous ovarian carcinoma progression. J Exp Clin Cancer Res 41:185 (2022). PubMed: 35624501

View all Publications for this product

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

KO cell lines

KO cell lysates

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (45)

Customer reviews and Q&As

Post-translational
modifications