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    products/primary-antibodies/iba1-antibody-ab5076.pdf

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Anti-Iba1 antibody (ab5076)

  • Datasheet
  • SDS
Reviews (100)Q&A (25)References (1145)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076)
  • Western blot - Anti-Iba1 antibody (ab5076)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076)

Key features and details

  • Goat polyclonal to Iba1
  • Suitable for: IHC-P, WB
  • Reacts with: Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Iba1 antibody [EPR16588] (ab178846)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Iba1 antibody
    See all Iba1 primary antibodies
  • Description

    Goat polyclonal to Iba1
  • Host species

    Goat
  • Specificity

    ab5076 is expected to recognize the isoforms represented by NP_001614 and NP_116573 but not NP_004838. 

    Although we received several excellent papers on ab5076 working with rat IHC-P, rat is not a batch tested species for IHC-P and we cannot guarantee.

  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Pig, Macaque monkey
  • Immunogen

    Synthetic peptide corresponding to Human Iba1 aa 135-147 (C terminal). Accession Number(s): NP_116573.1; NP_001614.3
    Sequence:

    C-TGPPAKKAISELP


    Database link: P55008
    (Peptide available as ab23067)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Human lymph node and rat brain tissue lysate. IHC-P: Human spleen tissue.
  • General notes

    The Iba1 antibody (ab5076) is commonly used as a marker of microglia activation in staining and immunohistochemistry, given that ionized calcium binding adaptor molecule 1 (Iba1) is a microglia/macrophage-specific calcium-binding protein with actin-bundling activity that participates in membrane ruffling and phagocytosis in activated microglia.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Immunology
    • Immune Interventions
    • Transplantation
    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Microglia marker
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Signal Transduction
    • Antibodies
    • iba1
    • Neuroscience
    • Development

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human Iba1 protein (ab105593)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
    • Anti-CD68 antibody [FA-11] (ab53444)
    • Anti-CD11b antibody [M1/70] (ab8878)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab5076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (28)
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Although we received several excellent papers on ab5076 working with rat IHC-P, rat is not a batch tested species for IHC-P and we cannot guarantee.

WB (7)
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 16-17 kDa (predicted molecular weight: 17 kDa).

A 1 hour primary incubation is recommended for this product. We received several excellent Abreviews on this antibody working with mouse, however, mouse is not a batch tested species and we cannot guarantee that this antibody will work on mouse. Some of our customers have observed high background in mouse samples. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. Blocking with BSA gives high background.

Notes
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Although we received several excellent papers on ab5076 working with rat IHC-P, rat is not a batch tested species for IHC-P and we cannot guarantee.

WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 16-17 kDa (predicted molecular weight: 17 kDa).

A 1 hour primary incubation is recommended for this product. We received several excellent Abreviews on this antibody working with mouse, however, mouse is not a batch tested species and we cannot guarantee that this antibody will work on mouse. Some of our customers have observed high background in mouse samples. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. Blocking with BSA gives high background.

Target

  • Function

    Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
  • Tissue specificity

    Detected in T-lymphocytes and peripheral blood mononuclear cells.
  • Sequence similarities

    Contains 2 EF-hand domains.
  • Post-translational
    modifications

    Phosphorylated on serine residues.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
  • Target information above from: UniProt accession P55008 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 199 Human
    • Entrez Gene: 397271 Pig
    • Entrez Gene: 29427 Rat
    • Omim: 601833 Human
    • SwissProt: P55008 Human
    • SwissProt: P81076 Pig
    • SwissProt: P55009 Rat
    • Unigene: 76364 Human
    • Unigene: 32080 Rat
    see all
  • Alternative names

    • AIF 1 antibody
    • AIF-1 antibody
    • Aif1 antibody
    • AIF1 protein antibody
    • AIF1_HUMAN antibody
    • Allograft inflammatory factor 1 antibody
    • Allograft inflammatory factor 1 splice variant G antibody
    • allograft inflammatory factor-1 splice variant Hara-1 antibody
    • balloon angioplasty responsive transcription antibody
    • BART 1 antibody
    • G1 antibody
    • G1 putative splice variant of allograft inflamatory factor 1 antibody
    • IBA 1 antibody
    • IBA1 antibody
    • interferon gamma responsive transcript antibody
    • Interferon responsive transcript 1 antibody
    • interferon responsive transcript factor 1 antibody
    • Ionized calcium binding adapter molecule 1 antibody
    • Ionized calcium-binding adapter molecule 1 antibody
    • ionized calcium-binding adapter molecule antibody
    • IRT 1 antibody
    • IRT1 antibody
    • Microglia response factor antibody
    • MRF1 antibody
    • Protein g1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling Iba1 with ab5076 at 8µg/ml.

    Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076)

    Negative control: Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling Iba1 with no primary antibody.

    Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.

  • Western blot - Anti-Iba1 antibody (ab5076)
    Western blot - Anti-Iba1 antibody (ab5076)
    All lanes : Anti-Iba1 antibody (ab5076) at 1 µg/ml

    Lane 1 : Human lymph node tissue lysate in RIPA buffer
    Lane 2 : Rat brain tissue lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 17 kDa



    Detected by chemiluminescence.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076)Image from Iris Margalit Trutzer et al, ACTA neuropathologica communications, 7, 40, Fig. 7; doi: 10.1186/s40478-019-0684-8

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lateral prefrontal cortex tissue labeling iba1 labeled microglia (labeled with black arrows) within the cortex using ab5076 at 1/1000 dilution. (Cropped image).

    Sections were rinsed in 0.01 m PBS, pH 7.4, followed by 10% serum matching the species of the secondary antibody, 5% bovine serum albumin, and 0.1% Triton X-100 in 0.01 m PBS blocking solution for 1 h and incubated for 2 days at 4 °C in primary antibody. The sections were rinsed in PBS and incubated overnight at 4 °C with a horse anti-Goat IgG (Biotinylated) secondary antibody.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1145)

Publishing research using ab5076? Please let us know so that we can cite the reference in this datasheet.

ab5076 has been referenced in 1145 publications.

  • McNamara NB  et al. Microglia regulate central nervous system myelin growth and integrity. Nature 613:120-129 (2023). PubMed: 36517604
  • Yuan X  et al. Spinal voltage-gated potassium channel Kv1.3 contributes to neuropathic pain via the promotion of microglial M1 polarization and activation of the NLRP3 inflammasome. Eur J Pain 27:289-302 (2023). PubMed: 36440534
  • Ijaz L  et al. Human Pluripotent Stem Cell Differentiation to Microglia. Methods Mol Biol 2454:455-471 (2022). PubMed: 33774810
  • Zhuang X  et al. Time- and Stimulus-Dependent Characteristics of Innate Immune Cells in Organ-Cultured Human Corneal Tissue. J Innate Immun 14:98-111 (2022). PubMed: 34182556
  • Augestad IL  et al. Normalisation of glucose metabolism by exendin-4 in the chronic phase after stroke promotes functional recovery in male diabetic mice. Br J Pharmacol 179:677-694 (2022). PubMed: 33973246
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-7 of 7 Abreviews or Q&A

Western blot abreview for Anti-Iba1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampus)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
40 µg
Specification
Hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Hyeon Joo Ham

Verified customer

Submitted Oct 23 2018

Western blot abreview for Anti-Iba1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Adult rat cerebellum (Ce) and pineal gland (PG))
Gel Running Conditions
Reduced Denaturing (20%)
Loading amount
40 µg
Specification
Adult rat cerebellum (Ce) and pineal gland (PG)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Estela Munoz

Verified customer

Submitted Dec 12 2016

Western blot abreview for Anti-Iba1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (P0 mouse cortex tissue)
Gel Running Conditions
Reduced Denaturing (12% Tris-Glycine)
Loading amount
50 µg
Specification
P0 mouse cortex tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ms. Xiaofeng Zhou

Verified customer

Submitted Jun 15 2016

Western blot abreview for Anti-Iba1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12% in MES)
Sample
Mouse Cell lysate - whole cell (Mouse Primary Microglia)
Specification
Mouse Primary Microglia
Blocking step
Li-Cor Block Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Grant Corbett

Verified customer

Submitted Jul 18 2014

Western blot abreview for Anti-Iba1 antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
50 µg
Specification
Brain
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 01 2013

Western blot abreview for Anti-Iba1 antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse retina whole cell lysate)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
mouse retina whole cell lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 27 2011

Western blot abreview for Anti-Iba1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (primary neurons)
Loading amount
30 µg
Specification
primary neurons
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 14 2010

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