Recombinant Anti-ICAM1 antibody [EPR16608] (ab179707)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR16608] (ab179707)](https://www.abcam.com/ps/products/179/ab179707/Images/ab179707-270806-anti-icam1-antibody-epr16608-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR16608] (ab179707)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16608] to ICAM1
- Suitable for: IHC-P, WB
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-ICAM1 antibody [EPR16608]
See all ICAM1 primary antibodies -
Description
Rabbit monoclonal [EPR16608] to ICAM1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse spleen, liver, kidney and lung lysates; bEnd.3, A20, RAW 264.7 and WEHI-231 whole cell lysates. IHC-P: Mouse liver and kidney tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16608 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (3) |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| WB | (1) |
1/1000. Detects a band of approximately 80-110 kDa (predicted molecular weight: 58 kDa).
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| Notes |
|---|
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/1000. Detects a band of approximately 80-110 kDa (predicted molecular weight: 58 kDa). |
Target
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Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. -
Sequence similarities
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsMonoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 15894 Mouse
- SwissProt: P13597 Mouse
- Unigene: 435508 Mouse
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Alternative names
- Antigen identified by monoclonal antibody BB2 antibody
- BB 2 antibody
- BB2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR16608] (ab179707)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling ICAM1 with ab179707 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Positive staining on endothelial cells of mouse liver is observed [PMID: 17058232].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR16608] (ab179707)](https://www.abcam.com/ps/products/179/ab179707/Images/ab179707-270805-anti-icam1-antibody-epr16608-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR16608] (ab179707)Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling ICAM1 with ab179707 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Positive staining on endothelial cells of mouse kidney is observed [PMID: 17058232].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-ICAM1 antibody [EPR16608] (ab179707)](https://www.abcam.com/ps/products/179/ab179707/Images/ab179707-270808-anti-icam1-antibody-epr16608-western-blot.jpg)
Western blot - Anti-ICAM1 antibody [EPR16608] (ab179707)All lanes : Anti-ICAM1 antibody [EPR16608] (ab179707) at 1/1000 dilution
Lane 1 : Mouse spleen lysate at 20 µg
Lane 2 : Mouse liver lysate at 20 µg
Lane 3 : Mouse kidney lysate at 20 µg
Lane 4 : bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate at 10 µg
Lane 5 : A20 (Mouse reticulum sarcoma cell line) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 58 kDa
Observed band size: 80-110 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The observed molecular weight is consistent with the literature (PMID: 1712683).
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![Western blot - Anti-ICAM1 antibody [EPR16608] (ab179707)](https://www.abcam.com/ps/products/179/ab179707/Images/ab179707-270807-anti-icam1-antibody-epr16608-western-blot.jpg)
Western blot - Anti-ICAM1 antibody [EPR16608] (ab179707)All lanes : Anti-ICAM1 antibody [EPR16608] (ab179707) at 1/1000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : WEHI-231 (Mouse lymphoblast B cell lymphoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 58 kDa
Observed band size: 80-110 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/2: 1 second; Lane 3: 10 seconds.
The observed molecular weight is consistent with the literature (PMID: 1712683).
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![Anti-ICAM1 antibody [EPR16608] (ab179707)](https://www.abcam.com/ps/products/179/ab179707/Images/ab179707-5-benefits-of-recombinant-antibodies.png)
Anti-ICAM1 antibody [EPR16608] (ab179707)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (20)
ab179707 has been referenced in 20 publications.
- Sun Y et al. The Role of Intestinal Dysbacteriosis Induced Arachidonic Acid Metabolism Disorder in Inflammaging in Atherosclerosis. Front Cell Infect Microbiol 11:618265 (2021). PubMed: 33816331
- Asad S et al. Proteomics-Informed Identification of Luminal Targets For In Situ Diagnosis of Inflammatory Bowel Disease. J Pharm Sci 110:239-250 (2021). PubMed: 33159915
- Li P et al. Inhibition of long noncoding RNA HIF1A-AS2 confers protection against atherosclerosis via ATF2 downregulation. J Adv Res 26:123-135 (2020). PubMed: 33133688
- Bai B et al. Deep Multilayer Brain Proteomics Identifies Molecular Networks in Alzheimer's Disease Progression. Neuron 105:975-991.e7 (2020). PubMed: 31926610
- Shrestha AK et al. Interactive and independent effects of early lipopolysaccharide and hyperoxia exposure on developing murine lungs. Am J Physiol Lung Cell Mol Physiol 319:L981-L996 (2020). PubMed: 32901520