Recombinant Anti-ICAM1 antibody [EPR19938] (ab206398)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR19938] (ab206398)](https://www.abcam.com/ps/products/206/ab206398/Images/ab206398-277609-anti-icam1-antibody-epr19938-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR19938] (ab206398)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19938] to ICAM1
- Suitable for: IHC-P, WB
- Reacts with: Rat
Related conjugates and formulations
Overview
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Product name
Anti-ICAM1 antibody [EPR19938]
See all ICAM1 primary antibodies -
Description
Rabbit monoclonal [EPR19938] to ICAM1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat spleen, lung, liver and kidney lysates; C6 and L6 whole cell lysates. IHC-P: Rat liver and kidney tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19938 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab206398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/5000. Detects a band of approximately 80-110 kDa (predicted molecular weight: 60 kDa).
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| Notes |
|---|
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/5000. Detects a band of approximately 80-110 kDa (predicted molecular weight: 60 kDa). |
Target
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Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. -
Sequence similarities
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsMonoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 25464 Rat
- SwissProt: Q00238 Rat
- Unigene: 12 Rat
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Alternative names
- Antigen identified by monoclonal antibody BB2 antibody
- BB 2 antibody
- BB2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR19938] (ab206398)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling ICAM1 with ab206398 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Membranous staining on sinusoidal endothelial cells of rat liver [PMID: 17058232].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR19938] (ab206398)](https://www.abcam.com/ps/products/206/ab206398/Images/ab206398-277606-anti-icam1-antibody-epr19938-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR19938] (ab206398)Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling ICAM1 with ab206398 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Positive staining on extracellular matrix and blood vessels of rat kidney [PMID: 9198039] [PMID 7902311].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-ICAM1 antibody [EPR19938] (ab206398)](https://www.abcam.com/ps/products/206/ab206398/Images/ab206398-277605-anti-icam1-antibody-epr19938-western-blot.jpg)
Western blot - Anti-ICAM1 antibody [EPR19938] (ab206398)All lanes : Anti-ICAM1 antibody [EPR19938] (ab206398) at 1/5000 dilution
Lane 1 : Rat spleen lysate at 20 µg
Lane 2 : Rat lung lysate at 20 µg
Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate at 10 µg
Lane 4 : L6 (Rat skeletal muscle cell line) whole cell lysate at 10 µg
Lane 5 : Rat liver lysate at 20 µg
Lane 6 : Rat kidney lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 60 kDa
Observed band size: 80-110 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-4: 5 seconds; Lanes 5/6: 1 minute.
The observed molecular weight is consistent with the literature (PMID: 1712683).
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![Anti-ICAM1 antibody [EPR19938] (ab206398)](https://www.abcam.com/ps/products/206/ab206398/Images/ab206398-5-benefits-of-recombinant-antibodies.png)
Anti-ICAM1 antibody [EPR19938] (ab206398)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab206398 has been referenced in 3 publications.
- Liu MM et al. Diammonium glycyrrhizinate lipid ligand ameliorates lipopolysaccharide-induced acute lung injury by modulating vascular endothelial barrier function. Exp Ther Med 21:303 (2021). PubMed: 33717246
- Xue J et al. The role of dendritic cells regulated by HMGB1/TLR4 signalling pathway in myocardial ischaemia reperfusion injury. J Cell Mol Med 23:2849-2862 (2019). PubMed: 30784177
- Haihua C et al. Cobra Venom Factor-induced complement depletion protects against lung ischemia reperfusion injury through alleviating blood-air barrier damage. Sci Rep 8:10346 (2018). PubMed: 29985461