Anti-IgA Secretory Component antibody [SC-05] (ab3924)

I have had a look at the nature of this protein: Extracellular matrix protein 1 (http://www.uniprot.org/uniprot/Q16610)

Unfortunately, I do not think it is scientifically to detect only ECM1b without detecting ECM1a.

The difference between these two isoforms is that ECM1b lacks amino acids 237-361. Therefore, if you had an antibody targeting this region, it would detect ECM1a but not ECM1b. You however wouldn't be able to specifically detect ECM1b as all the amino acids present in this isoform are also present in ECM1a.

It appears that there is a nomenclature difference between your target of interest (secretory component) and our antibodies that are anti "secretory component glycoprotein" which is also referred to as "Extracellular matrix protein 1" and "secretory component p85"
Thus, it seems an anti-PIGR antibody would be more applicable for your needs. Even though an anti-PIGR antibody that only detects the cleaved secretory component without detecting uncleaved PIGR won't exist, we can find one that detects both cleaved secretory component and the uncleaved, full-length PIGR by choosing an antibody that recognizes aa 19-603 of PIGR. Our rabbit polyclonal anti-PIGR antibody that meets this requirement is listed below:
ab96196
https://www.abcam.com/pigr-antibody-ab96196.html

For ab3924, the links to the databases and target information mention "Extracellular matrix protein 1" which is an alternative name for "secretory component p85" and "secretory component glycoprotein" according to the uniprot file below:
http://www.uniprot.org/uniprot/Q16610

Thank you for contacting us.

In general, the selection of the immunogen varies from antibody to antibody. Some antibodies are raised against synthetic peptides (based ona protein sequence which was derived originally from a DNA sequence), some against protein fragments and others against the full length protein (either purified or overexpressed in another organism) or a cell preparation.

Which specific antibody or protein target do you have in mind?

As for the anti-IgA antibodies - the immunogendepends on the antibody.

Please let me know more details about the products of your interest and I'd be happy to assist you further.

Thank you for taking the time to respond and for contacting Abcam.

With over 70,000 products it is logistically impossible for us to offer free samples. I hope you understand.

Please let me know if you have any questions.

Thank you for contacting Abcam.

I am sorry to hear that Ab55676 has not performed well for you. Although the peptide sequence has excellent identity with the mouse homolog, this product is not guaranteed to work in mouse tissues. High background is a very common issue when working in tissues of the same species as the host speciesof the primary anti-body. These may be reduced by using a secondary antibody that has been pre-absorbed or by direct conjugation of your label to the primary antibody.

Our EasyLink conjugation kits offer an effective and simple way to directly conjugate labels to your primary antibody. More information may be found at the following link:

https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink

We have 12 secondary antibodies against mouse IgG1, they may be viewed at the following link:

https://www.abcam.com/index.html?pageconfig=searchresults&search=IgG1&pt=7&sk=targsp&sv=2&sn=Mouse&l=2&fViewMore=1

Please let me know if you have any questions.

Thank you for contacting us. Unfortunately we do not have any specific information about the epitope of Anti-Secretory Component Glycoprotein antibody [SC-05] (ab3924). We do however know that the epitope recognised is reduction-resistant and present in both the free and Ig-bound secretory component. More precise information regarding the epitope is not known.   I hope this information has been of help. If you need any further information please let us know and we will try to assist you. 

Thank you for your enquiry. Unfortunately the only information the originator of the antibody was able to provide was mostly on the datasheet: The antibody reacts with 80 kDa human secretory component glycoprotein (both free and bound in secretory IgA). Recognized glycoprotein is specific membrane marker of glandular carcinomas. I am sorry I could not be more helpful. Please do not hesitate to contact us if you need anything further.

Thank you for your enquiry. There is no information currently available about the working dilution for this antibody in IHC and Western blot. For a purified antibody, we recommend starting dilutions for Western blot to be 1 ug/mL and for IHC, 10 ug/mL. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.