Recombinant Anti-IGF1 Receptor + Insulin Receptor antibody [EPR2334(2)] (ab172965)
![Immunoprecipitation - Anti-IGF1 Receptor + Insulin Receptor antibody [EPR2334(2)] (ab172965)](https://www.abcam.com/ps/products/172/ab172965/Images/ab172965-196304-anti-igf1-receptor-insulin-receptor-antibody-epr23342-immunoprecipitation.jpg "Immunoprecipitation - Anti-IGF1 Receptor + Insulin Receptor antibody [EPR2334(2)] (ab172965)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2334(2)] to IGF1 Receptor + Insulin Receptor
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IGF1 Receptor + Insulin Receptor antibody [EPR2334(2)]
See all IGF1 Receptor+Insulin Receptor primary antibodies -
Description
Rabbit monoclonal [EPR2334(2)] to IGF1 Receptor + Insulin Receptor -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 293T, HeLa, SK-OV-3, A549 cell lysates, fetal brain lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2334(2) -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 156 kDa.
|
| IP |
1/10 - 1/100.
|
| Notes |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 156 kDa. |
|
IP
1/10 - 1/100. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
Target
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Cellular localization
IGF1 Receptor: Cell membrane. Insulin Receptor: Cell membrane. -
Database links
- Entrez Gene: 3480 Human
- Entrez Gene: 3643 Human
- Entrez Gene: 16001 Mouse
- Entrez Gene: 16337 Mouse
- Entrez Gene: 24954 Rat
- Entrez Gene: 25718 Rat
- Omim: 147370 Human
- Omim: 147670 Human
see all
Images
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Immunoprecipitation - Anti-IGF1 Receptor + Insulin Receptor antibody [EPR2334(2)] (ab172965)
Western blot analysis on immunoprecipitation pellet from 293T cell lysate using ab172965.
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![Western blot - Anti-IGF1 Receptor + Insulin Receptor antibody [EPR2334(2)] (ab172965)](https://www.abcam.com/ps/products/172/ab172965/Images/ab172965-196302-anti-igf1-receptor-insulin-receptor-antibody-epr23342-western-blot.jpg)
Western blot - Anti-IGF1 Receptor + Insulin Receptor antibody [EPR2334(2)] (ab172965)All lanes : Anti-IGF1 Receptor + Insulin Receptor antibody [EPR2334(2)] (ab172965) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SK-OV-3 cell lysate
Lane 4 : A459 cell lysate
Lane 5 : Fetal brain lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 156 kDa -
![Anti-IGF1 Receptor + Insulin Receptor antibody [EPR2334(2)] (ab172965)](https://www.abcam.com/ps/products/172/ab172965/Images/ab172965-3-benefits-of-recombinant-antibodies.png)
Anti-IGF1 Receptor + Insulin Receptor antibody [EPR2334(2)] (ab172965)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab172965 has been referenced in 2 publications.
- Yang P et al. Liraglutide ameliorates nonalcoholic fatty liver disease in diabetic mice via the IRS2/PI3K/Akt signaling pathway. Diabetes Metab Syndr Obes 12:1013-1021 (2019). PubMed: 31308717
- Tang J et al. Continuous exposure of non-small cell lung cancer cells with wild-type EGFR to an inhibitor of EGFR tyrosine kinase induces chemoresistance by activating STAT3. Int J Oncol 46:2083-95 (2015). PubMed: 25695284