Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-IGF1R (pY1161) + INSR (pY1185) + INSRR (pY1141) antibody [EPR26123-29] (ab314116)

Submit a review Submit a question
Western blot - Anti-IGF1R (pY1161) + INSR (pY1185) + INSRR (pY1141) antibody [EPR26123-29] (ab314116)
  • Western blot - Anti-IGF1R (pY1161) + INSR (pY1185) + INSRR (pY1141) antibody [EPR26123-29] (ab314116)
  • Immunoprecipitation - Anti-IGF1R (pY1161) + INSR (pY1185) + INSRR (pY1141) antibody [EPR26123-29] (ab314116)
  • Dot Blot - Anti-IGF1R (pY1161) + INSR (pY1185) + INSRR (pY1141) antibody [EPR26123-29] (ab314116)
  • Anti-IGF1R (pY1161) + INSR (pY1185) + INSRR (pY1141) antibody [EPR26123-29] (ab314116)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26123-29] to IGF1 Receptor (phospho Y1161) + Insulin Receptor (phospho Y1185) + IRR (phospho Y1141)
  • Suitable for: WB, IP, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-IGF1R (pY1161) + INSR (pY1185) + INSRR (pY1141) antibody [EPR26123-29]

  • Description

    Rabbit monoclonal [EPR26123-29] to IGF1 Receptor (phospho Y1161) + Insulin Receptor (phospho Y1185) + IRR (phospho Y1141)

  • Host species

    Rabbit

  • Specificity

    Based on dot blot data using peptides, this antibody reacts with IGF1 Receptor (phospho Y1161), Insulin receptor (phospho Y1185) and Insulin receptor-related protein (phospho Y1141).

  • Tested applications

    Suitable for: WB, IP, Dot blotmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa starved for 18 hours, then treated with 10ng/ml IGF-1 for 10 minutes whole cell lysate, HeLa starved for 18 hours, then treated with 10ng/ml IGF-1 for 5 minutes and HeLa starved for 18 hours, then treated with 10ng/ml IGF-1 for 20 minutes whole cell lysate IP: HeLa starved for 18 hours, then treated with 10ng/ml IGF-1 for 20 minutes whole cell lysate. Dot blot: IGF1 receptor (phospho Y1161)/Insulin receptor (phospho Y1185) peptide, Insulin receptor-related protein (phospho Y1141) peptide

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab314116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000.
IP
1/30.
Dot blot
1/1000.
Notes
WB
1/1000.
IP
1/30.
Dot blot
1/1000.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

Target

Images

  • Western blot - Anti-IGF1R (pY1161) + INSR (pY1185) + INSRR (pY1141) antibody [EPR26123-29] (ab314116)
    Western blot - Anti-IGF1R (pY1161) + INSR (pY1185) + INSRR (pY1141) antibody [EPR26123-29] (ab314116)
    All lanes : Anti-IGF1R (pY1161) + INSR (pY1185) + INSRR (pY1141) antibody [EPR26123-29] (ab314116) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) starved for 18 hours whole cell lysate (untreated membrane)
    Lane 2 : HeLa starved for 18 hours, then treated with 10ng/ml IGF-1 for 10 minutes whole cell lysate (untreated membrane)
    Lane 3 : HeLa starved for 18 hours whole cell lysate (alkaline phosphatase treated membrane)
    Lane 4 : HeLa starved for 18 hours, then treated with 10ng/ml IGF-1 for 10 minutes whole cell lysate (alkaline phosphatase treated membrane)

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Observed band size: 100 kDa why is the actual band size different from the predicted?


    Exposure time: 81 seconds


    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This blot was developed using a high sensitivity ECL substrate.

    In Western blot, Anti-IGF1 Receptor antibody [EPR19322] (ab182408) staining at 1/1000 dilution

    Exposure time: 81 seconds

  • Western blot - Anti-IGF1R (pY1161) + INSR (pY1185) + INSRR (pY1141) antibody [EPR26123-29] (ab314116)
    Western blot - Anti-IGF1R (pY1161) + INSR (pY1185) + INSRR (pY1141) antibody [EPR26123-29] (ab314116)
    Lanes 1 & 3 : Anti-IGF1R (pY1161) + INSR (pY1185) + INSRR (pY1141) antibody [EPR26123-29] (ab314116) at 1/1000 dilution
    Lane 2 : Anti-IGF1R (pY1161) + INSR (pY1185) + INSRR (pY1141) antibody [EPR26123-29] (ab314116)

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) starved for 18 hours whole cell lysate
    Lane 2 : HeLa starved for 18 hours, then treated with 10ng/ml IGF-1 for 5 minutes whole cell lysate
    Lane 3 : HeLa starved for 18 hours, then treated with 10ng/ml IGF-1 for 20 minutes whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Observed band size: 100 kDa why is the actual band size different from the predicted?


    Exposure time: 92 seconds


    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This blot was developed using a high sensitivity ECL substrate.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-IGF1 Receptor antibody [EPR19322] (ab182408) staining at 1/1000 dilution

    Exposure time: 92 seconds

  • Immunoprecipitation - Anti-IGF1R (pY1161) + INSR (pY1185) + INSRR (pY1141) antibody [EPR26123-29] (ab314116)
    Immunoprecipitation - Anti-IGF1R (pY1161) + INSR (pY1185) + INSRR (pY1141) antibody [EPR26123-29] (ab314116)

    IGF1R (pY1161) + INSR (pY1185) + INSRR (pY1141) was immunoprecipitated from 0.35 mg HeLa starved for 18 hours, then treated with 10ng/ml IGF-1 for 20 minutes whole cell lysate with ab314116 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314116 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa starved for 18 hours, then treated with 10ng/ml IGF-1 for 20 minutes whole cell lysate

    Lane 2: ab314116 IP in HeLa starved for 18 hours, then treated with 10ng/ml IGF-1 for 20 minutes whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab314116 in HeLa starved for 18 hours, then treated with 10ng/ml IGF-1 for 20 minutes whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 180 seconds

  • Dot Blot - Anti-IGF1R (pY1161) + INSR (pY1185) + INSRR (pY1141) antibody [EPR26123-29] (ab314116)
    Dot Blot - Anti-IGF1R (pY1161) + INSR (pY1185) + INSRR (pY1141) antibody [EPR26123-29] (ab314116)

    Dot blot analysis of IGF1R (pY1161) + INSR (pY1185) + INSRR (pY1141) using ab314116 at 1:1000 (0.521 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.

    Lane 1: IGF1 receptor (phospho Y1161)/Insulin receptor (phospho Y1185) peptide

    Lane 2: IGF1 receptor/Insulin receptor non-phospho peptide

    Lane 3: Insulin receptor-related protein (phospho Y1141) peptide

    Lane 4: IGF1 receptor (phospho Y1165) peptide

    Lane 5: IGF1 receptor (phospho Y1166) peptide

    Lane 6: IGF1 receptor (phospho T1163) peptide

    Exposure time: 180 seconds

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

  • Anti-IGF1R (pY1161) + INSR (pY1185) + INSRR (pY1141) antibody [EPR26123-29] (ab314116)
    Anti-IGF1R (pY1161) + INSR (pY1185) + INSRR (pY1141) antibody [EPR26123-29] (ab314116)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab314116? Please let us know so that we can cite the reference in this datasheet.

ab314116 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab314116.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com