Recombinant Anti-IGJ antibody [SP105] (ab105229)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229)](https://www.abcam.com/ps/products/105/ab105229/Images/ab105229-4-AntiIGJ-antibody-SP105-IHC-human-stomach.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP105] to IGJ
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IGJ antibody [SP105]
See all IGJ primary antibodies -
Description
Rabbit monoclonal [SP105] to IGJ -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human IGJ aa 50-150 (internal sequence). The exact sequence is proprietary.
Database link: P01591 -
Positive control
- IHC-P: Human tonsil and stomach tissue Flow Cyt: Daudi cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP105 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/20.
|
|
| IHC-P | (1) |
1/100. Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue section in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
|
| Notes |
|---|
|
Flow Cyt
1/20. |
|
IHC-P
1/100. Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue section in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
Target
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Function
Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory component. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3512 Human
- Entrez Gene: 16069 Mouse
- Omim: 147790 Human
- SwissProt: P01591 Human
- SwissProt: P01592 Mouse
- Unigene: 643431 Human
- Unigene: 1192 Mouse
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Alternative names
- IGCJ antibody
- IGJ_HUMAN antibody
- Immunoglobulin J chain antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling IGJ with ab105229 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the stromal cells of human stomach, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab105229 for 10 mins at room temperature. -
![Flow Cytometry - Anti-IGJ antibody [SP105] (ab105229)](https://www.abcam.com/ps/products/105/ab105229/Images/ab105229-2-ab105229FC1.jpg)
Flow Cytometry - Anti-IGJ antibody [SP105] (ab105229)Flow cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) labeling IGJ with purified ab105229 at 1/20 dilution (5.3 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229)](https://www.abcam.com/ps/products/105/ab105229/Images/ab105229-3-AntiIGJ-antibody-SP105-IHC-human-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling IGJ with ab105229 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab105229 for 10 mins at room temperature. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229)](https://www.abcam.com/ps/products/105/ab105229/Images/ab105229-117743-anti-igj-antibody-sp105-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [SP105] (ab105229)ab105229, at 1/100 dilution, staining IGJ in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry. -
![Anti-IGJ antibody [SP105] (ab105229)](https://www.abcam.com/ps/products/105/ab105229/Images/ab105229-5-benefits-of-recombinant-antibodies.png)
Anti-IGJ antibody [SP105] (ab105229)
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab105229 has not yet been referenced specifically in any publications.