Anti-IGLL1 antibody (ab154517)
Key features and details
- Rabbit polyclonal to IGLL1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IGLL1 antibody -
Description
Rabbit polyclonal to IGLL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 130-213 of Human IGLL1 (Uniprot ID: P15814).
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Positive control
- THP1 whole cell lysate; Human colon epithelium lymphocyte tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 23 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 10mM Citrate buffer
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 23 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 10mM Citrate buffer |
Target
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Function
Critical for B-cell development. -
Tissue specificity
Expressed only in pre-B-cells and a special B-cell line (which is surface Ig negative). -
Involvement in disease
Defects in IGLL1 are the cause of agammaglobulinemia type 2 (AGM2) [MIM:613500]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. -
Sequence similarities
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3543 Human
- Omim: 146770 Human
- SwissProt: P15814 Human
- Unigene: 348935 Human
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Alternative names
- 14.1 antibody
- AGM2 antibody
- CD179 antigen-like family member B antibody
see all
Images
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Western blot - Anti-IGLL1 antibody (ab154517)Anti-IGLL1 antibody (ab154517) at 1/1000 dilution + THP1 whole cell lysate at 30 µg
Predicted band size: 23 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGLL1 antibody (ab154517)Immunohistochemical analysis of paraffin-embedded Human colon epithelium lymphocyte tissue labeling IGLL1 with ab154517 at 1/250 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154517 has not yet been referenced specifically in any publications.