Recombinant Anti-IL-1 alpha antibody [EPR25263-3] (ab300501)
![Western blot - Anti-IL-1 alpha antibody [EPR25263-3] (AB300501)](https://www.abcam.com/ps/products/300/ab300501/Images/AB300501-486411-rabbit-monoclonal-epr25263-3-to-il-1-alpha-western-blot.jpg "Western blot - Anti-IL-1 alpha antibody [EPR25263-3] (AB300501)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25263-3] to IL-1 alpha
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-IL-1 alpha antibody [EPR25263-3]
See all IL-1 alpha primary antibodies -
Description
Rabbit monoclonal [EPR25263-3] to IL-1 alpha -
Host species
Rabbit -
Specificity
Human species reactivity is recommended based on IHC results, we do not guarantee human species reactivity in WB.
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Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details
Unsuitable for: Flow Cyt (Intra) or IP -
Species reactivity
Reacts with: Mouse, Human
Does not react with: Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: RAW264.7 treated with 10µg/ml lipopolysaccharide (LPS) for 7 hours, whole cell lysate IHC-P: Mouse lung treated with lipopolysaccharides (1 µg/ml) for 16 h in vitro, human colon, and human colon cancer. ICC/IF: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 10 ug/ml lipopolysaccharide
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR25263-3 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab300501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
Human species reactivity is recommended based on IHC results, we do not guarantee human species reactivity in WB. |
|
| ICC/IF |
1/50.
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| IHC-P | (1) |
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). Human species reactivity is recommended based on IHC results, we do not guarantee human species reactivity in WB. |
|
ICC/IF
1/50. |
|
IHC-P
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. -
Sequence similarities
Belongs to the IL-1 family. -
Domain
The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function. -
Cellular localization
Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. - Information by UniProt
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Database links
- Entrez Gene: 3552 Human
- Entrez Gene: 16175 Mouse
- Omim: 147760 Human
- SwissProt: P01583 Human
- SwissProt: P01582 Mouse
- Unigene: 1722 Human
- Unigene: 15534 Mouse
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Alternative names
- BAF antibody
- FAF antibody
- Hematopoietin 1 antibody
see all
Images
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Western blot - Anti-IL-1 alpha antibody [EPR25263-3] (AB300501)All lanes : Anti-IL-1 alpha antibody [EPR25263-3] (ab300501) at 1/1000 dilution
Lane 1 : Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 2 : RAW264.7 treated with 10µg/ml lipopolysaccharide (LPS) for 7 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 15 secondsBlocking and dilution buffer and concentration: 5% NFDM/TBST.
The expression level of IL-1 alpha was upregulated by LPS stimulation (PMID: 25870118).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody [EPR25263-3] (AB300501)](https://www.abcam.com/ps/products/300/ab300501/Images/AB300501-486420-YCAImmunohistochemistry-rabbit-monoclonal-epr252633-to-il1-alpha-mouse-lung-lps.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody [EPR25263-3] (AB300501)Immunohistochemical analysis of paraffin-embedded A: Mouse lung treated with lipopolysaccharides (1 μg/ml) for 16 h in vitro. B: Untreated mouse lung labelling IL-1 alpha with ab300501 at 1/5000 dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse lung induced by LPS (A) and no staining on untreated mouse lung (B). The section was incubated with ab300501 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins is used.
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![Immunocytochemistry/ Immunofluorescence - Anti-IL-1 alpha antibody [EPR25263-3] (ab300501)](https://www.abcam.com/ps/products/300/ab300501/Images/AB300501-486433-immunocytochemistry-rabbit-monoclonal-epr252633-to-il1-alpha-raw2647-lps.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IL-1 alpha antibody [EPR25263-3] (ab300501)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized treated or untreated with 10 μg/ml lipopolysaccharide for 24h RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate labeling IL-1 alpha with ab300501 at 1/50 dilution, followed by (ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 dilution (green). Confocal image showing increased nuclear staining in Raw 264.7 cells treated with lipopolysaccharide (10 μg/ml) for 24 h (PMID:25870118). The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594, ab195889 ) at 1/200 dilution (red).
Secondary antibody only controls: PBS was used instead of primary antibody in lipopolysacchride treated or untreated for 24h RAW 264.7 followed by preadsorbed (ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution and nuclear stained with DAPI.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody [EPR25263-3] (AB300501)](https://www.abcam.com/ps/products/300/ab300501/Images/AB300501-486429-immunohistochemistry-rabbit-monoclonal-epr252633-to-il1-alpha-human-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody [EPR25263-3] (AB300501)Immunohistochemical analysis of paraffin-embedded human colon tissue labelling IL-1 alpha with ab300501 at 1/5000 dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Cytoplasmic staining on immune cells of human colon is observed. The section was incubated with ab300501 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins is used.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody [EPR25263-3] (AB300501)](https://www.abcam.com/ps/products/300/ab300501/Images/AB300501-486431-immunohistochemistry-rabbit-monoclonal-epr252633-to-il1-alpha-human-colon-cancer.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody [EPR25263-3] (AB300501)Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labelling IL-1 alpha with ab300501 at 1/5000 dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Cytoplasmic staining on immune cells of human colon cancer is observed. The section was incubated with ab300501 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins is used.
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![Anti-IL-1 alpha antibody [EPR25263-3] (AB300501)](https://www.abcam.com/ps/products/300/ab300501/Images/AB300501-486409-Benefits-of-Recombinant-antibodies.jpg)
Anti-IL-1 alpha antibody [EPR25263-3] (AB300501)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab300501 has not yet been referenced specifically in any publications.