Anti-IL-10 antibody [4A7-25-17] (ab134742)
![Sandwich ELISA - Anti-IL-10 antibody [4A7-25-17] (ab134742)](https://www.abcam.com/ps/products/134/ab134742/Images/ab134742-453245-anti-il-10-antibody-4a7-25-17-sandwich-elisa.jpg "Sandwich ELISA - Anti-IL-10 antibody [4A7-25-17] (ab134742)")
Key features and details
- Mouse monoclonal [4A7-25-17] to IL-10
- Suitable for: Sandwich ELISA, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-IL-10 antibody [4A7-25-17]
See all IL-10 primary antibodies -
Description
Mouse monoclonal [4A7-25-17] to IL-10 -
Host species
Mouse -
Tested applications
Suitable for: Sandwich ELISA, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human IL-10.
-
Positive control
- WB: Recombinant Human IL-10 protein.
-
General notes
Although some customers have been successful in IHC we no longer batch test in this application.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Lyophilized:This product can be reconstituted to a final concentration of 1 mg/ml in water. -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
4A7-25-17 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA |
Use a concentration of 8 - 10 µg/ml. Can be paired for Sandwich ELISA with Biotin Rabbit polyclonal to IL-10 (ab84031).
8.0-10.0 µg/ml of this antibody will detect at least 1000 pg/well of recombinant Human IL-10. As the detection antibody at a concentration of approximately 0.5-1.0 µg/ml. |
|
| IHC-P | (2) |
Use at an assay dependent concentration.
Customer feedback suggests heat-mediated antigen retrieval with Tris-EDTA pH9.0 is effective. |
| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 20 kDa.
Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions. We recommend 3% Nonfat Dry Milk in diH2O as a blocking solution |
| Notes |
|---|
|
Sandwich ELISA
Use a concentration of 8 - 10 µg/ml. Can be paired for Sandwich ELISA with Biotin Rabbit polyclonal to IL-10 (ab84031). 8.0-10.0 µg/ml of this antibody will detect at least 1000 pg/well of recombinant Human IL-10. As the detection antibody at a concentration of approximately 0.5-1.0 µg/ml. |
|
IHC-P
Use at an assay dependent concentration. Customer feedback suggests heat-mediated antigen retrieval with Tris-EDTA pH9.0 is effective. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 20 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions. We recommend 3% Nonfat Dry Milk in diH2O as a blocking solution |
Target
-
Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 3586 Human
- Omim: 124092 Human
- SwissProt: P22301 Human
- Unigene: 193717 Human
-
Alternative names
- CSIF antibody
- Cytokine synthesis inhibitory factor antibody
- GVHDS antibody
see all
Images
-
Sandwich ELISA - Anti-IL-10 antibody [4A7-25-17] (ab134742)
In a sandwich ELISA (assuming 100 μL/well), a concentration of 8.0-10.0 μg/mL of ab134742 will detect at least 2000 pg/mL of Recombinant Human IL-10 when used with Biotin Anti-IL-10 antibody (ab84031) as the detection antibody at a concentration of approximately 0.05-0.10 μg/mL.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (11)
ab134742 has been referenced in 11 publications.
- Zhao M et al. Cancer-Associated Fibroblast-Derived Exosomal miRNA-320a Promotes Macrophage M2 Polarization In Vitro by Regulating PTEN/PI3Kγ Signaling in Pancreatic Cancer. J Oncol 2022:9514697 (2022). PubMed: 35813857
- Liu J et al. Prediction and identification of CD4+ T cell epitope for the protective antigens of Mycobacterium tuberculosis. Medicine (Baltimore) 100:e24619 (2021). PubMed: 33578573
- De Luccia TPB et al. The effect of Gestational Diabetes Mellitus on the fetal compartment. J Reprod Immunol 145:103314 (2021). PubMed: 33836321
- Bi CS et al. The relationship between T-helper cell polarization and the RANKL/OPG ratio in gingival tissues from chronic periodontitis patients. Clin Exp Dent Res 5:377-388 (2019). PubMed: 31944625
- Zhao Y et al. In vitro neutralization of autocrine IL-10 affects Op18/stathmin signaling in non-small cell lung cancer cells. Oncol Rep 41:501-511 (2019). PubMed: 30320402
