Recombinant Anti-IL-10 antibody [EP1115Y] (ab52909)
![Western blot - Anti-IL-10 antibody [EP1115Y] (ab52909)](https://www.abcam.com/ps/products/52/ab52909/Images/ab52909-65396-anti-il-10-antibody-ep1115y-western-blot.jpg "Western blot - Anti-IL-10 antibody [EP1115Y] (ab52909)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1115Y] to IL-10
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IL-10 antibody [EP1115Y]
See all IL-10 primary antibodies -
Description
Rabbit monoclonal [EP1115Y] to IL-10 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human IL-10 aa 100-200 (N terminal). The exact sequence is proprietary.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1115Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52909 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
This has only been tested against the recombinant protein. |
| Notes |
|---|
|
WB
1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). This has only been tested against the recombinant protein. |
Target
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Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3586 Human
- Omim: 124092 Human
- SwissProt: P22301 Human
- Unigene: 193717 Human
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Alternative names
- CSIF antibody
- Cytokine synthesis inhibitory factor antibody
- GVHDS antibody
see all
Images
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Western blot - Anti-IL-10 antibody [EP1115Y] (ab52909)Anti-IL-10 antibody [EP1115Y] (ab52909) at 1/5000 dilution + Recombinant protein (5ng)
Secondary
Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa -
![Anti-IL-10 antibody [EP1115Y] (ab52909)](https://www.abcam.com/ps/products/52/ab52909/Images/ab52909-2-benefits-of-recombinant-antibodies.png)
Anti-IL-10 antibody [EP1115Y] (ab52909)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab52909 has been referenced in 3 publications.
- Fitzpatrick CJ et al. Junin Virus Activates p38 MAPK and HSP27 Upon Entry. Front Cell Infect Microbiol 12:798978 (2022). PubMed: 35463647
- Abbasi M et al. Proteomic Analysis of Cardioembolic and Large Artery Atherosclerotic Clots Using Reverse Phase Protein Array Technology Reveals Key Cellular Interactions Within Clot Microenvironments. Cureus 13:e13499 (2021). PubMed: 33777584
- de la Fuente C et al. Phosphoproteomic analysis reveals Smad protein family activation following Rift Valley fever virus infection. PLoS One 13:e0191983 (2018). PubMed: 29408900