Recombinant Anti-IL-10 antibody [EPR1114] (ab133575)
![Flow Cytometry (Intracellular) - Anti-IL-10 antibody [EPR1114] (ab133575)](https://www.abcam.com/ps/products/133/ab133575/Images/ab133575-253473-anti-il-10-antibody-epr1114-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Anti-IL-10 antibody [EPR1114] (ab133575)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1114] to IL-10
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IL-10 antibody [EPR1114]
See all IL-10 primary antibodies -
Description
Rabbit monoclonal [EPR1114] to IL-10 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
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Positive control
- Flow Cyt (intra): HeLa cells; Human PBMCs. WB: recombinant IL-10 protein. ICC/IF: Human peripheral blood mononuclear cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.70 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1114 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | (1) |
1/50 - 1/250.
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| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).
This application will not work using endogenous samples. It reacts only with the recombinant fragment. |
| Notes |
|---|
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF
1/50 - 1/250. |
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WB
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa). This application will not work using endogenous samples. It reacts only with the recombinant fragment. |
Target
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Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3586 Human
- Omim: 124092 Human
- SwissProt: P22301 Human
- Unigene: 193717 Human
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Alternative names
- CSIF antibody
- Cytokine synthesis inhibitory factor antibody
- GVHDS antibody
see all
Images
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Flow Cytometry (Intracellular) - Anti-IL-10 antibody [EPR1114] (ab133575)
Overlay histogram showing HeLa cells fixed in 4% PFA and stained with purified ab133575 at a dilution of 1 in 300 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
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![Immunocytochemistry/ Immunofluorescence - Anti-IL-10 antibody [EPR1114] (ab133575)](https://www.abcam.com/ps/products/133/ab133575/Images/ab133575-234801-anti-il-10-antibody-epr1114-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IL-10 antibody [EPR1114] (ab133575)This image is courtesy of an anonymous AbreviewUnpurified ab133575 staining IL-10 in human peripheral blood mononuclear cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Saponin in PBS and blocked with 4% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in blocking buffer) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-IL-10 antibody [EPR1114] (ab133575)](https://www.abcam.com/ps/products/133/ab133575/Images/ab133575-253464-anti-il-10-antibody-epr1114-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IL-10 antibody [EPR1114] (ab133575)Immunofluorescence staining of Molt-4 cells with purified ab133575 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab133575 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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![Western blot - Anti-IL-10 antibody [EPR1114] (ab133575)](https://www.abcam.com/ps/products/133/ab133575/Images/ab133575-147357-anti-il-10-antibody-epr1114-western-blot.jpg)
Western blot - Anti-IL-10 antibody [EPR1114] (ab133575)Anti-IL-10 antibody [EPR1114] (ab133575) at 1/1000 dilution (unpurified) + Recombinant IL-10 protein at 0.005 µg
Secondary
Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-IL-10 antibody [EPR1114] (ab133575)](https://www.abcam.com/ps/products/133/ab133575/Images/ab133575-147356-anti-il-10-antibody-epr1114-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-IL-10 antibody [EPR1114] (ab133575)Intracellular Flow Cytometry analysis of permeabilized HeLa cells labelling IL-10 using unpurified ab133575 at a 1/100 dilution (red) or a control rabbit IgG antibody (green).
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![OI-RD Scanning - Anti-IL-10 antibody [EPR1114] (ab133575)](https://www.abcam.com/ps/products/133/ab133575/Images/ab133575-147355-anti-il-10-antibody-epr1114.jpg)
OI-RD Scanning - Anti-IL-10 antibody [EPR1114] (ab133575) -
![Flow Cytometry (Intracellular) - Anti-IL-10 antibody [EPR1114] (ab133575)](https://www.abcam.com/ps/products/133/ab133575/Images/ab133575-294301-anti-il-10-antibody-epr1114-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-IL-10 antibody [EPR1114] (ab133575)This image is courtesy of an anonymous Abreview.Intracellular flow cytometric analysis ofhuman PBMCs (peripheral blood mononuclear cells)treated with PHA labeling IL-10 with ab133575 at 1/100 dilution (green), followed by Goat anti-rabbit Alexa Fluorr® 488.
1 (dashed line) = isotype control, 2 (black line) = PBMCs untreated, 3 (green line) = PBMCs treated with PHA.
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![Anti-IL-10 antibody [EPR1114] (ab133575)](https://www.abcam.com/ps/products/133/ab133575/Images/ab133575-10-benefits-of-recombinant-antibodies.png)
Anti-IL-10 antibody [EPR1114] (ab133575)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab133575 has been referenced in 9 publications.
- Chan L et al. Cytokine Profile in Plasma Extracellular Vesicles of Parkinson's Disease and the Association with Cognitive Function. Cells 10:N/A (2021). PubMed: 33803292
- Zhu H et al. Knockdown of RSAD2 attenuates B cell hyperactivity in patients with primary Sjögren's syndrome (pSS) via suppressing NF-?b signaling pathway. Mol Cell Biochem 476:2029-2037 (2021). PubMed: 33512636
- Zhang T et al. GPR18 Agonist Resolvin D2 Reduces Early Brain Injury in a Rat Model of Subarachnoid Hemorrhage by Multiple Protective Mechanisms. Cell Mol Neurobiol N/A:N/A (2021). PubMed: 34089427
- Zhang P et al. Lactobacillus acidophilus ATCC 4356 Alleviates Renal Ischemia-Reperfusion Injury Through Antioxidant Stress and Anti-inflammatory Responses and Improves Intestinal Microbial Distribution. Front Nutr 8:667695 (2021). PubMed: 34046422
- Toyama N et al. The effect of macrophages on an atmospheric pressure plasma-treated titanium membrane with bone marrow stem cells in a model of guided bone regeneration. J Mater Sci Mater Med 31:70 (2020). PubMed: 32705350
- Chen L et al. IL-10 secreted by cancer-associated macrophages regulates proliferation and invasion in gastric cancer cells via c-Met/STAT3 signaling. Oncol Rep 42:595-604 (2019). PubMed: 31233202
- Zhai GT et al. IgD-activated mast cells induce IgE synthesis in B cells in nasal polyps. J Allergy Clin Immunol 142:1489-1499.e23 (2018). PubMed: 30102935
- Keuper M et al. Activated macrophages control human adipocyte mitochondrial bioenergetics via secreted factors. Mol Metab 6:1226-1239 (2017). PubMed: 29031722
- Raha AA et al. Neuroprotective Effect of TREM-2 in Aging and Alzheimer's Disease Model. J Alzheimers Dis N/A:N/A (2016). IHC ; Mouse . PubMed: 27662313