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    products/primary-antibodies/il-10-antibody-epr1114-ab133575.pdf

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Immunology Innate Immunity Cytokines Interleukins
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RecombinantRabMAb

Recombinant Anti-IL-10 antibody [EPR1114] (ab133575)

  • Datasheet
  • SDS
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Flow Cytometry (Intracellular) - Anti-IL-10 antibody [EPR1114] (ab133575)
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-10 antibody [EPR1114] (ab133575)
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-10 antibody [EPR1114] (ab133575)
  • Western blot - Anti-IL-10 antibody [EPR1114] (ab133575)
  • Flow Cytometry (Intracellular) - Anti-IL-10 antibody [EPR1114] (ab133575)
  • OI-RD Scanning - Anti-IL-10 antibody [EPR1114] (ab133575)
  • Flow Cytometry (Intracellular) - Anti-IL-10 antibody [EPR1114] (ab133575)
  • Anti-IL-10 antibody [EPR1114] (ab133575)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1114] to IL-10
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 APC Carrier Free PE PerCP

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Overview

  • Product name

    Anti-IL-10 antibody [EPR1114]
    See all IL-10 primary antibodies
  • Description

    Rabbit monoclonal [EPR1114] to IL-10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IL-10 aa 1-100. The exact sequence is proprietary.
    Database link: P22301
    (Peptide available as ab169704)

  • Positive control

    • Flow Cyt (intra): HeLa cells; Human PBMCs. WB: recombinant IL-10 protein. ICC/IF: Human peripheral blood mononuclear cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 3.70 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1114
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • PE Anti-IL-10 antibody [EPR1114] (ab209930)
    • Anti-IL-10 antibody [EPR1114] - BSA and Azide free (ab215975)
    • PerCP Anti-IL-10 antibody [EPR1114] (ab220518)
    • APC Anti-IL-10 antibody [EPR1114] (ab221277)
    • Anti-IL-10 antibody [EPR1114] - Low endotoxin, Azide free (ab246688)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Ramos whole cell lysate (ab3955)
    • THP-1 whole cell lysate (ab7913)
  • Recombinant Protein

    • Recombinant human IL-10 protein (Active) (ab259402)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF (1)
1/50 - 1/250.
WB (1)
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).

This application will not work using endogenous samples. It reacts only with the recombinant fragment.

Notes
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/50 - 1/250.
WB
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).

This application will not work using endogenous samples. It reacts only with the recombinant fragment.

Application notes
Is unsuitable for IHC-P.

Target

  • Function

    Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Tissue specificity

    Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • Sequence similarities

    Belongs to the IL-10 family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P22301 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3586 Human
    • Omim: 124092 Human
    • SwissProt: P22301 Human
    • Unigene: 193717 Human
    • Alternative names

      • CSIF antibody
      • Cytokine synthesis inhibitory factor antibody
      • GVHDS antibody
      • IL 10 antibody
      • IL-10 antibody
      • IL10 antibody
      • IL10_HUMAN antibody
      • IL10A antibody
      • Interleukin 10 antibody
      • Interleukin-10 antibody
      • MGC126450 antibody
      • MGC126451 antibody
      • T-cell growth inhibitory factor antibody
      • TGIF antibody
      see all

    Images

    • Flow Cytometry (Intracellular) - Anti-IL-10 antibody [EPR1114] (ab133575)
      Flow Cytometry (Intracellular) - Anti-IL-10 antibody [EPR1114] (ab133575)

      Overlay histogram showing HeLa cells fixed in 4% PFA and stained with purified ab133575 at a dilution of 1 in 300 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).

       

    • Immunocytochemistry/ Immunofluorescence - Anti-IL-10 antibody [EPR1114] (ab133575)
      Immunocytochemistry/ Immunofluorescence - Anti-IL-10 antibody [EPR1114] (ab133575)This image is courtesy of an anonymous Abreview

      Unpurified ab133575 staining IL-10 in human peripheral blood mononuclear cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Saponin in PBS and blocked with 4% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in blocking buffer) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-IL-10 antibody [EPR1114] (ab133575)
      Immunocytochemistry/ Immunofluorescence - Anti-IL-10 antibody [EPR1114] (ab133575)

      Immunofluorescence staining of Molt-4 cells with purified ab133575 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab133575 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    • Western blot - Anti-IL-10 antibody [EPR1114] (ab133575)
      Western blot - Anti-IL-10 antibody [EPR1114] (ab133575)
      Anti-IL-10 antibody [EPR1114] (ab133575) at 1/1000 dilution (unpurified) + Recombinant IL-10 protein at 0.005 µg

      Secondary
      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 20 kDa
      Observed band size: 18 kDa why is the actual band size different from the predicted?

    • Flow Cytometry (Intracellular) - Anti-IL-10 antibody [EPR1114] (ab133575)
      Flow Cytometry (Intracellular) - Anti-IL-10 antibody [EPR1114] (ab133575)

      Intracellular Flow Cytometry analysis of permeabilized HeLa cells labelling IL-10 using unpurified ab133575 at a 1/100 dilution (red) or a control rabbit IgG antibody (green). 

       

       

    • OI-RD Scanning - Anti-IL-10 antibody [EPR1114] (ab133575)
      OI-RD Scanning - Anti-IL-10 antibody [EPR1114] (ab133575)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Flow Cytometry (Intracellular) - Anti-IL-10 antibody [EPR1114] (ab133575)
      Flow Cytometry (Intracellular) - Anti-IL-10 antibody [EPR1114] (ab133575)This image is courtesy of an anonymous Abreview.

      Intracellular flow cytometric analysis ofhuman PBMCs (peripheral blood mononuclear cells)treated with PHA labeling IL-10 with ab133575 at 1/100 dilution (green), followed by Goat anti-rabbit Alexa Fluorr® 488. 

      1 (dashed line) = isotype control, 2 (black line) = PBMCs untreated, 3 (green line) = PBMCs treated with PHA. 

       

       

      See Abreview

    • Anti-IL-10 antibody [EPR1114] (ab133575)
      Anti-IL-10 antibody [EPR1114] (ab133575)

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (9)

    Publishing research using ab133575? Please let us know so that we can cite the reference in this datasheet.

    ab133575 has been referenced in 9 publications.

    • Chan L  et al. Cytokine Profile in Plasma Extracellular Vesicles of Parkinson's Disease and the Association with Cognitive Function. Cells 10:N/A (2021). PubMed: 33803292
    • Zhu H  et al. Knockdown of RSAD2 attenuates B cell hyperactivity in patients with primary Sjögren's syndrome (pSS) via suppressing NF-?b signaling pathway. Mol Cell Biochem 476:2029-2037 (2021). PubMed: 33512636
    • Zhang T  et al. GPR18 Agonist Resolvin D2 Reduces Early Brain Injury in a Rat Model of Subarachnoid Hemorrhage by Multiple Protective Mechanisms. Cell Mol Neurobiol N/A:N/A (2021). PubMed: 34089427
    • Zhang P  et al. Lactobacillus acidophilus ATCC 4356 Alleviates Renal Ischemia-Reperfusion Injury Through Antioxidant Stress and Anti-inflammatory Responses and Improves Intestinal Microbial Distribution. Front Nutr 8:667695 (2021). PubMed: 34046422
    • Toyama N  et al. The effect of macrophages on an atmospheric pressure plasma-treated titanium membrane with bone marrow stem cells in a model of guided bone regeneration. J Mater Sci Mater Med 31:70 (2020). PubMed: 32705350
    • Chen L  et al. IL-10 secreted by cancer-associated macrophages regulates proliferation and invasion in gastric cancer cells via c-Met/STAT3 signaling. Oncol Rep 42:595-604 (2019). PubMed: 31233202
    • Zhai GT  et al. IgD-activated mast cells induce IgE synthesis in B cells in nasal polyps. J Allergy Clin Immunol 142:1489-1499.e23 (2018). PubMed: 30102935
    • Keuper M  et al. Activated macrophages control human adipocyte mitochondrial bioenergetics via secreted factors. Mol Metab 6:1226-1239 (2017). PubMed: 29031722
    • Raha AA  et al. Neuroprotective Effect of TREM-2 in Aging and Alzheimer's Disease Model. J Alzheimers Dis N/A:N/A (2016). IHC ; Mouse . PubMed: 27662313

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-IL-10 antibody [EPR1114]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Permeabilization
    Yes - 0.1 M PBS with 3% Triton X
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Aug 20 2015

    Flow Cytometry abreview for Anti-IL-10 antibody [EPR1114]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (PBMCs (peripheral blood mononuclear cells))
    Permeabilization
    Yes - 0,1% Saponin /1% FCS/ PBS
    Gating Strategy
    no gate
    Specification
    PBMCs (peripheral blood mononuclear cells)
    Preparation
    Cell harvesting/tissue preparation method: isolated by gradient centrifugation
    Sample buffer: 0,1% Saponin /1% FCS/ PBS
    Fixation
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 27 2014

    Western blot abreview for Anti-IL-10 antibody [EPR1114]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Recombinant protein (see below)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (5-16% gel)
    Loading amount
    10 µg
    Specification
    see below
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 27 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-IL-10 antibody [EPR1114]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (PBMCs (peripheral blood mononuclear cells))
    Permeabilization
    Yes - 0,1% Saponin/PBS
    Specification
    PBMCs (peripheral blood mononuclear cells)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 22 2014

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