Anti-IL-10 antibody [JES5-2A5] (ab189392)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (ab189392)](https://www.abcam.com/ps/products/189/ab189392/Images/ab189392-224167-anti-il-10-antibody-jes5-2a5-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (ab189392)")
Key features and details
- Rat monoclonal [JES5-2A5] to IL-10
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG1
Overview
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Product name
Anti-IL-10 antibody [JES5-2A5]
See all IL-10 primary antibodies -
Description
Rat monoclonal [JES5-2A5] to IL-10 -
Host species
Rat -
Specificity
ab189392 recognizes mouse IL-10. IL-10 is expressed by Ly-1 (B-1) B cells that express CD5 (Ly-1) and a subset of lectin stimulated T cells. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment corresponding to Mouse IL-10.
Database link: P18893 -
Positive control
- Mouse small intestine and spleen tissues. Recombinant mouse IL-10.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
JES5-2A5 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 21 kDa.
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| IHC-P | (2) |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000. Predicted molecular weight: 21 kDa. |
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 16153 Mouse
- SwissProt: P18893 Mouse
- Unigene: 874 Mouse
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Alternative names
- CSIF antibody
- Cytokine synthesis inhibitory factor antibody
- GVHDS antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (ab189392)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse small intestine tissue, labeling IL-10 using ab189392 at 10 µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (ab189392)](https://www.abcam.com/ps/products/189/ab189392/Images/ab189392-224168-anti-il-10-antibody-jes5-2a5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (ab189392)Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse spleen tissue, labeling IL-10 using ab189392 at 10 µg/ml.
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![Western blot - Anti-IL-10 antibody [JES5-2A5] (ab189392)](https://www.abcam.com/ps/products/189/ab189392/Images/ab189392-224169-anti-il-10-antibody-jes5-2a5-western-blot.jpg)
Western blot - Anti-IL-10 antibody [JES5-2A5] (ab189392)Anti-IL-10 antibody [JES5-2A5] (ab189392) at 1/1000 dilution + recombinant mouse IL-10
Secondary
F(ab)2 rabbit anti-rat IgG:HRP
Developed using the ECL technique.
Predicted band size: 21 kDa
Protocols
Datasheets and documents
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Datasheet download
References (33)
ab189392 has been referenced in 33 publications.
- Chen Y et al. PP1A prevents ROS-induced pyroptosis by inhibiting MAPK/caspase-3 in mouse adipose tissue. FEBS J 289:3839-3853 (2022). PubMed: 35080339
- Zeng P et al. Qing Zao Fang (QZF) Alleviates the Inflammatory Microenvironment of the Submandibular Gland in Sjögren's Syndrome Based on the PI3K/Akt/HIF-1α/VEGF Signaling Pathway. Dis Markers 2022:6153459 (2022). PubMed: 35140821
- Huang C et al. Up-Regulated Expression of Interferon-Gamma, Interleukin-6 and Tumor Necrosis Factor-Alpha in the Endolymphatic Sac of Meniere's Disease Suggesting the Local Inflammatory Response Underlies the Mechanism of This Disease. Front Neurol 13:781031 (2022). PubMed: 35280304
- Li J et al. S100A9-CXCL12 activation in BRCA1-mutant breast cancer promotes an immunosuppressive microenvironment associated with resistance to immunotherapy. Nat Commun 13:1481 (2022). PubMed: 35304461
- Wang G et al. Icariin alleviates uveitis by targeting peroxiredoxin 3 to modulate retinal microglia M1/M2 phenotypic polarization. Redox Biol 52:102297 (2022). PubMed: 35334248
