Recombinant Anti-IL-12A antibody [EP5737] (ab131039)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] (ab131039)](https://www.abcam.com/ps/products/131/ab131039/Images/ab131039-260497-anti-il-12a-antibody-ep5737-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] (ab131039)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP5737] to IL-12A
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-IL-12A antibody [EP5737]
See all IL-12A primary antibodies -
Description
Rabbit monoclonal [EP5737] to IL-12A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- NIH3T3, HuT-78, and Raw 264.7 cell lysates; Human kidney tissue, Human gastric carcinoma, Human colon tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP5737 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
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| IHC-P | (1) |
1/800. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
| Flow Cyt (Intra) |
1/19000.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa. |
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IHC-P
1/800. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
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Flow Cyt (Intra)
1/19000. |
Target
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Function
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3592 Human
- Entrez Gene: 16159 Mouse
- Omim: 161560 Human
- SwissProt: P29459 Human
- SwissProt: P43431 Mouse
- Unigene: 673 Human
- Unigene: 103783 Mouse
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Alternative names
- CLMF antibody
- CLMF p35 antibody
- Cytotoxic lymphocyte maturation factor 1 p35 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] (ab131039)
Immunohistochemical staining of paraffin embedded human gastric carcinoma tissue section labelling IL-12A with purified ab131039 at dilution of 1/800. The secondary antibody used was HRP conjugated Goat Anti-Rabbit IgG H&L (ab97051) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
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![Flow Cytometry (Intracellular) - Anti-IL-12A antibody [EP5737] (ab131039)](https://www.abcam.com/ps/products/131/ab131039/Images/ab131039-330898-anti-il-12a-antibody-ep5737-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-IL-12A antibody [EP5737] (ab131039)Intracellular Flow Cytometry analysis of Raw264.7 cells treated with 100ng/ml for LPS 24hour cells labelling IL-12A with ab131039 at 1/19000 dilution (1.02 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).
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![Flow Cytometry (Intracellular) - Anti-IL-12A antibody [EP5737] (ab131039)](https://www.abcam.com/ps/products/131/ab131039/Images/ab131039-330897-anti-il-12a-antibody-ep5737-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-IL-12A antibody [EP5737] (ab131039)Intracellular Flow Cytometry analysis of THP-1 cells treated with 100ng/ml PMA for 30min,then 1µg/ml LPS for 24hour cells labelling IL-12A with ab131039 at 1/19000 dilution (1.02µg/ml) (Red). Cells were fixed with 4% paraformaldehyde . Dylight488® (Ab98462) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue). Untreated cells (Green).
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![Western blot - Anti-IL-12A antibody [EP5737] (ab131039)](https://www.abcam.com/ps/products/131/ab131039/Images/ab131039-260484-anti-il-12a-antibody-ep5737-western-blot.jpg)
Western blot - Anti-IL-12A antibody [EP5737] (ab131039)Anti-IL-12A antibody [EP5737] (ab131039) at 1/1000 dilution (purified) + HuT-78 (human Sezary syndrome) whole cell lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer 5% NFDM/TBST -
![Western blot - Anti-IL-12A antibody [EP5737] (ab131039)](https://www.abcam.com/ps/products/131/ab131039/Images/ab131039-260483-anti-il-12a-antibody-ep5737-western-blot.jpg)
Western blot - Anti-IL-12A antibody [EP5737] (ab131039)All lanes : Anti-IL-12A antibody [EP5737] (ab131039) at 1/5000 dilution (purified)
Lane 1 : NIH/3T3 (mouse embryo) whole cell lysate
Lane 2 : Raw264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer 5% NFDM/TBST -
![Western blot - Anti-IL-12A antibody [EP5737] (ab131039)](https://www.abcam.com/ps/products/131/ab131039/Images/ab131039-145254-anti-il-12a-antibody-ep5737-western-blot.jpg)
Western blot - Anti-IL-12A antibody [EP5737] (ab131039)All lanes : Anti-IL-12A antibody [EP5737] (ab131039) at 1/1000 dilution (unpurified)
Lane 1 : NIH3T3 cell lysate
Lane 2 : HuT-78 cell lysate
Lane 3 : Raw 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] (ab131039)](https://www.abcam.com/ps/products/131/ab131039/Images/ab131039-145253-anti-il-12a-antibody-ep5737-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] (ab131039)Immunohistochemical analysis of paraffin-embedded human kidney tissue sections labelling IL-12A with unpurified ab131039 at dilution of 1:250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] (ab131039)](https://www.abcam.com/ps/products/131/ab131039/Images/ab131039-294303-anti-il-12a-antibody-ep5737-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] (ab131039)This image is courtesy of an anonymous Abreview.Formaldehyde-fixed, paraffin-embedded human colon tissue stained for IL-12A using ab131039 at 1/500 dilution in immunohistochemical analysis.
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![Anti-IL-12A antibody [EP5737] (ab131039)](https://www.abcam.com/ps/products/131/ab131039/Images/ab131039-11-benefits-of-recombinant-antibodies.png)
Anti-IL-12A antibody [EP5737] (ab131039)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab131039 has been referenced in 7 publications.
- Luchtel RA et al. High-dose ascorbic acid synergizes with anti-PD1 in a lymphoma mouse model. Proc Natl Acad Sci U S A 117:1666-1677 (2020). PubMed: 31911474
- Zhang GQ et al. High Expression of Interleukin-12A and Its Association with the Clinicopathology and Prognosis of Differentiated Thyroid Cancer. Eur Thyroid J 9:139-147 (2020). PubMed: 32523890
- Larousserie F et al. Evidence for IL-35 Expression in Diffuse Large B-Cell Lymphoma and Impact on the Patient's Prognosis. Front Oncol 9:563 (2019). PubMed: 31316915
- Liu L et al. Periductal Mastitis: An Inflammatory Disease Related to Bacterial Infection and Consequent Immune Responses? Mediators Inflamm 2017:5309081 (2017). PubMed: 28182101
- Zhang Y et al. The Unknown Aspect of BAFF: Inducing IL-35 Production by a CD5+CD1dhiFc?RIIbhiRegulatory B-Cell Subset in Lupus. J Invest Dermatol 137:2532-2543 (2017). WB . PubMed: 28844943
- Sarmento-Cabral A et al. Metformin Reduces Prostate Tumor Growth, in a Diet-Dependent Manner, by Modulating Multiple Signaling Pathways. Mol Cancer Res 15:862-874 (2017). PubMed: 28385910
- Terayama H et al. Contribution of IL-12/IL-35 common subunit p35 to maintaining the testicular immune privilege. PLoS One 9:e96120 (2014). IHC-Fr ; Mouse . PubMed: 24760014