Recombinant Anti-IL-12B antibody [EPR5739] (ab133752)
![Immunocytochemistry/ Immunofluorescence - Anti-IL-12B antibody [EPR5739] (ab133752)](https://www.abcam.com/ps/products/133/ab133752/Images/ab133752-5-ab133752ICCIF1.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IL-12B antibody [EPR5739] (ab133752)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5739] to IL-12B
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IL-12B antibody [EPR5739]
See all IL-12B primary antibodies -
Description
Rabbit monoclonal [EPR5739] to IL-12B -
Host species
Rabbit -
Specificity
This antibody is unsuitable to detect endogenous IL-12B in WB.
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Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human IL-12B aa 300-400. The exact sequence is proprietary.
Database link: P29460 -
Positive control
- WB: Human IL-12B recombinant protein. ICC/IF: MOLT-4 cells Flow Cyt (intra): Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.30 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5739 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/300.
|
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| WB |
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).
This antibody is unsuitable to detect endogenous IL12B in WB |
|
| ICC/IF |
1/100 - 1/250.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/300. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa). This antibody is unsuitable to detect endogenous IL12B in WB |
|
ICC/IF
1/100 - 1/250. |
Target
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Function
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. -
Involvement in disease
Mendelian susceptibility to mycobacterial disease
Psoriasis 11 -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 3 subfamily.
Contains 1 fibronectin type-III domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Post-translational
modificationsKnown to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3593 Human
- Omim: 161561 Human
- SwissProt: P29460 Human
- Unigene: 674 Human
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Alternative names
- CLMF antibody
- CLMF p40 antibody
- CLMF2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-IL-12B antibody [EPR5739] (ab133752)
Immunocytochemistry/ Immunofluorescence analysis of MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling IL-12B with Purified ab133752 at 1:100 dilution (3.3 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were not counterstained. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Western blot - Anti-IL-12B antibody [EPR5739] (ab133752)](https://www.abcam.com/ps/products/133/ab133752/Images/ab133752-339736-ab133752WB1.jpg)
Western blot - Anti-IL-12B antibody [EPR5739] (ab133752)All lanes : Anti-IL-12B antibody [EPR5739] (ab133752) at 1/1000 dilution (Purified)
All lanes : Human IL-12 recombinant protein
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?The recombinant protein contains Human IL-12B (Ile23-Ser328) and Human IL-12A (Arg23-Ser219) connected by disulfide bond to be heterodimer. Under reducing conditions, the observed bands will be at 40 KD for IL-12B and 29 KD for IL-12A.
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![Western blot - Anti-IL-12B antibody [EPR5739] (ab133752)](https://www.abcam.com/ps/products/133/ab133752/Images/ab133752-147842-anti-il-12b-antibody-epr5739-western-blot.jpg)
Western blot - Anti-IL-12B antibody [EPR5739] (ab133752)Anti-IL-12B antibody [EPR5739] (ab133752) at 1/1000 dilution + Human IL-12B recombinant protein at 0.01 µg
Secondary
Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?This image was generated using the unpurified version of the product.
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![Flow Cytometry (Intracellular) - Anti-IL-12B antibody [EPR5739] (ab133752)](https://www.abcam.com/ps/products/133/ab133752/Images/ab133752-280383-anti-il-12b-antibody-epr5739-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-IL-12B antibody [EPR5739] (ab133752)Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling IL-12B (red) with ab133752 at a 1/2500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
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![OI-RD Scanning - Anti-IL-12B antibody [EPR5739] (ab133752)](https://www.abcam.com/ps/products/133/ab133752/Images/ab133752-147841-anti-il-12b-antibody-epr5739.jpg)
OI-RD Scanning - Anti-IL-12B antibody [EPR5739] (ab133752) -
![Anti-IL-12B antibody [EPR5739] (ab133752)](https://www.abcam.com/ps/products/133/ab133752/Images/ab133752-6-benefits-of-recombinant-antibodies.png)
Anti-IL-12B antibody [EPR5739] (ab133752)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab133752 has been referenced in 4 publications.
- Bohnacker S et al. Influence of glycosylation on IL-12 family cytokine biogenesis and function. Mol Immunol 126:120-128 (2020). PubMed: 32823236
- Meier S et al. The molecular basis of chaperone-mediated interleukin 23 assembly control. Nat Commun 10:4121 (2019). PubMed: 31511508
- Reitberger S et al. Assembly-induced folding regulates interleukin 12 biogenesis and secretion. J Biol Chem 292:8073-8081 (2017). PubMed: 28325840
- Marks E et al. Regulation of IL-12p40 by HIF controls Th1/Th17 responses to prevent mucosal inflammation. Mucosal Immunol 10:1224-1236 (2017). PubMed: 28120851