Recombinant Anti-IL-17F antibody [EPR17830-169] (ab187059)
![Flow Cytometry (Intracellular) - Anti-IL-17F antibody [EPR17830-169] (ab187059)](https://www.abcam.com/ps/products/187/ab187059/Images/ab187059-308549-anti-il-17f-antibody-epr17830-169-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Anti-IL-17F antibody [EPR17830-169] (ab187059)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17830-169] to IL-17F
- Suitable for: Flow Cyt (Intra), IP, WB
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-IL-17F antibody [EPR17830-169]
See all IL-17F primary antibodies -
Description
Rabbit monoclonal [EPR17830-169] to IL-17F -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IP, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse IL17F recombinant protein (aa29-161) ; EL4 cell lysate (treated with PMA, Ionomycin calcuim salt and Brefeldin A). Flow Cyt (intra): EL4 cells treated with PMA, Ionomycin calcuim salt and Brefeldin A. IP: EL4 cells treated with PMA, Ionomycin calcuim salt and Brefeldin A.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17830-169 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/60.
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| IP |
1/30.
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| WB |
1/1000. Predicted molecular weight: 18 kDa.
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| Notes |
|---|
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Flow Cyt (Intra)
1/60. |
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IP
1/30. |
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WB
1/1000. Predicted molecular weight: 18 kDa. |
Target
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Function
Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover (PubMed:11591732, PubMed:11591768, PubMed:11574464). Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (PubMed:11591732). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation. -
Tissue specificity
Expressed in activated, but not resting, CD4+ T-cells and activated monocytes. -
Involvement in disease
Candidiasis, familial, 6 -
Sequence similarities
Belongs to the IL-17 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 257630 Mouse
- SwissProt: Q7TNI7 Mouse
- Unigene: 222807 Mouse
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Alternative names
- CANDF6 antibody
- Cytokine ML 1 antibody
- Cytokine ML-1 antibody
see all
Images
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Flow Cytometry (Intracellular) - Anti-IL-17F antibody [EPR17830-169] (ab187059)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol-fixed EL4 (mouse lymphoma T lymphocyte) treated with 50ng/ml Phorbol-12-myristate-13-acetate (PMA) and 500ng/ml Ionomycin calcium salt for 24 hours, and 500ng/ml Brefeldin A (BFA) was added for the last 20 hours (Right) / Untreated control (Left) labeling IL17F with ab187059 at 1/60 dilution.
Secondary antibody used Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1/2000 dilution.
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![Western blot - Anti-IL-17F antibody [EPR17830-169] (ab187059)](https://www.abcam.com/ps/products/187/ab187059/Images/ab187059-308546-anti-il-17f-antibody-epr17830-169-western-blot.jpg)
Western blot - Anti-IL-17F antibody [EPR17830-169] (ab187059)All lanes : Anti-IL-17F antibody [EPR17830-169] (ab187059) at 1/5000 dilution
Lane 1 : Mouse IL17A recombinant protein (aa26-158) 10 ng
Lane 2 : Mouse IL17F recombinant protein (aa29-161) 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 26 secondsBlocking/Dilution buffer: 5% NFDM/TBST
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![Immunoprecipitation - Anti-IL-17F antibody [EPR17830-169] (ab187059)](https://www.abcam.com/ps/products/187/ab187059/Images/ab187059-308548-anti-il-17f-antibody-epr17830-169-immunoprecipitation.jpg)
Immunoprecipitation - Anti-IL-17F antibody [EPR17830-169] (ab187059)IL17F was immunoprecipitated from 0.35 mg of EL4 (mouse lymphoma T lymphocyte) treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate with ab187059 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187059 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5,000 dilution.
Lane 1: EL4 (mouse lymphoma T lymphocyte) treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate 10 μg (Input).
Lane 2: EL4 treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187059 in EL4 treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate.Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds
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![Western blot - Anti-IL-17F antibody [EPR17830-169] (ab187059)](https://www.abcam.com/ps/products/187/ab187059/Images/ab187059-308547-anti-il-17f-antibody-epr17830-169-western-blot.jpg)
Western blot - Anti-IL-17F antibody [EPR17830-169] (ab187059)All lanes : Anti-IL-17F antibody [EPR17830-169] (ab187059) at 1/1000 dilution
Lane 1 : Untreated EL4 (mouse lymphoma T lymphocyte), whole cell lysate
Lane 2 : EL4 treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added to the treated cells last 20 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 18 kDa
Observed band size: 16-18 kDa why is the actual band size different from the predicted?
Exposure time: 48 secondsBlocking/Diluting buffer: 5% NFDM/TBST
The molecular mass observed is consistent with the literature (PMID 2212322).
Expression of IL-17F in EL4 cells is increased by PMA and Ionomycin treatment (PMID 28382171).
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![Anti-IL-17F antibody [EPR17830-169] (ab187059)](https://www.abcam.com/ps/products/187/ab187059/Images/ab187059-5-benefits-of-recombinant-antibodies.png)
Anti-IL-17F antibody [EPR17830-169] (ab187059)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab187059 has not yet been referenced specifically in any publications.