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RecombinantRabMAb

Recombinant Anti-IL-17F antibody [EPR17830-169] (ab187059)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Flow Cytometry (Intracellular) - Anti-IL-17F antibody [EPR17830-169] (ab187059)
  • Western blot - Anti-IL-17F antibody [EPR17830-169] (ab187059)
  • Immunoprecipitation - Anti-IL-17F antibody [EPR17830-169] (ab187059)
  • Western blot - Anti-IL-17F antibody [EPR17830-169] (ab187059)
  • Anti-IL-17F antibody [EPR17830-169] (ab187059)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17830-169] to IL-17F
  • Suitable for: Flow Cyt (Intra), IP, WB
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
Anti-KLRG1 antibody [2F1] (ab25054)

View more associated products

Overview

  • Product name

    Anti-IL-17F antibody [EPR17830-169]
    See all IL-17F primary antibodies
  • Description

    Rabbit monoclonal [EPR17830-169] to IL-17F
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), IP, WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse IL17F recombinant protein (aa29-161) ; EL4 cell lysate (treated with PMA, Ionomycin calcuim salt and Brefeldin A). Flow Cyt (intra): EL4 cells treated with PMA, Ionomycin calcuim salt and Brefeldin A. IP: EL4 cells treated with PMA, Ionomycin calcuim salt and Brefeldin A.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17830-169
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Inhibitors
    • Cardiovascular
    • Angiogenesis
    • Cytokines
    • Interleukin
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic inhibitory factors

Associated products

  • Alternative Versions

    • Anti-IL-17F antibody [EPR17830-169] - BSA and Azide free (ab234532)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    • Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab187059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/60.
IP
1/30.
WB
1/1000. Predicted molecular weight: 18 kDa.
Notes
Flow Cyt (Intra)
1/60.
IP
1/30.
WB
1/1000. Predicted molecular weight: 18 kDa.

Target

  • Function

    Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover (PubMed:11591732, PubMed:11591768, PubMed:11574464). Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (PubMed:11591732). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.
  • Tissue specificity

    Expressed in activated, but not resting, CD4+ T-cells and activated monocytes.
  • Involvement in disease

    Candidiasis, familial, 6
  • Sequence similarities

    Belongs to the IL-17 family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q96PD4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 257630 Mouse
    • SwissProt: Q7TNI7 Mouse
    • Unigene: 222807 Mouse
    • Alternative names

      • CANDF6 antibody
      • Cytokine ML 1 antibody
      • Cytokine ML-1 antibody
      • IL 17F antibody
      • IL 24 antibody
      • IL-17F antibody
      • IL-24 antibody
      • Il17f antibody
      • IL17F_HUMAN antibody
      • Interleukin 17F antibody
      • Interleukin 24 antibody
      • Interleukin-17F antibody
      • Interleukin-24 antibody
      • ML 1 antibody
      • ML1 antibody
      • Mutant IL 17F antibody
      • OTTHUMP00000016602 antibody
      see all

    Images

    • Flow Cytometry (Intracellular) - Anti-IL-17F antibody [EPR17830-169] (ab187059)
      Flow Cytometry (Intracellular) - Anti-IL-17F antibody [EPR17830-169] (ab187059)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol-fixed EL4 (mouse lymphoma T lymphocyte) treated with 50ng/ml Phorbol-12-myristate-13-acetate (PMA) and 500ng/ml Ionomycin calcium salt for 24 hours, and 500ng/ml Brefeldin A (BFA) was added for the last 20 hours (Right) / Untreated control (Left) labeling IL17F with ab187059 at 1/60 dilution. 

      Secondary antibody used Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1/2000 dilution. 

       

       

    • Western blot - Anti-IL-17F antibody [EPR17830-169] (ab187059)
      Western blot - Anti-IL-17F antibody [EPR17830-169] (ab187059)
      All lanes : Anti-IL-17F antibody [EPR17830-169] (ab187059) at 1/5000 dilution

      Lane 1 : Mouse IL17A recombinant protein (aa26-158) 10 ng
      Lane 2 : Mouse IL17F recombinant protein (aa29-161) 10 ng

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 18 kDa
      Observed band size: 18 kDa


      Exposure time: 26 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST

    • Immunoprecipitation - Anti-IL-17F antibody [EPR17830-169] (ab187059)
      Immunoprecipitation - Anti-IL-17F antibody [EPR17830-169] (ab187059)

      IL17F was immunoprecipitated from 0.35 mg of EL4 (mouse lymphoma T lymphocyte) treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate with ab187059 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187059 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5,000 dilution.

      Lane 1: EL4 (mouse lymphoma T lymphocyte) treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate 10 μg (Input).
      Lane 2: EL4 treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187059 in EL4 treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: 10 seconds

    • Western blot - Anti-IL-17F antibody [EPR17830-169] (ab187059)
      Western blot - Anti-IL-17F antibody [EPR17830-169] (ab187059)
      All lanes : Anti-IL-17F antibody [EPR17830-169] (ab187059) at 1/1000 dilution

      Lane 1 : Untreated EL4 (mouse lymphoma T lymphocyte), whole cell lysate
      Lane 2 : EL4 treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added to the treated cells last 20 hours, whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 18 kDa
      Observed band size: 16-18 kDa why is the actual band size different from the predicted?


      Exposure time: 48 seconds


      Blocking/Diluting buffer: 5% NFDM/TBST

      The molecular mass observed is consistent with the literature (PMID 2212322).

      Expression of IL-17F in EL4 cells is increased by PMA and Ionomycin treatment (PMID 28382171).

    • Anti-IL-17F antibody [EPR17830-169] (ab187059)
      Anti-IL-17F antibody [EPR17830-169] (ab187059)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab187059? Please let us know so that we can cite the reference in this datasheet.

    ab187059 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Mass Cytometry abreview for Anti-IL-17F antibody [EPR17830-169]

    Below Average
    Abreviews
    Abreviews
    Application
    Mass Cytometry
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 01 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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