Name: Anti-IL-18BP antibody [EP1088Y]
Brand: RabMAb
SKU: ab52914
Rating: 3 (1 reviews)

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Western blot - Anti-IL-18BP antibody [EP1088Y] (ab52914)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1088Y] to IL-18BP
Suitable for: WB, IHC-P
Reacts with: Rat, Human

Carrier Free

Product name

Anti-IL-18BP antibody [EP1088Y]
See all IL-18BP primary antibodies
Description

Rabbit monoclonal [EP1088Y] to IL-18BP
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivity

Reacts with: Rat, Human
Immunogen

Synthetic peptide within Human IL-18BP aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: O95998
Positive control
- WB: HeLa and K562 cell lysates and rat kidney and heart tissue lysates. IHC-P: human tonsil tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1088Y
Isotype

IgG
Research areas

Alternative Versions
- Anti-IL-18BP antibody [EP1088Y] - BSA and Azide free (ab239852)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
Recombinant Protein
- Recombinant Human IL-18BP (ab193078)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(1)	1/1000 - 1/5000. Predicted molecular weight: 22 kDa.
IHC-P		1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.

Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 22 kDa.
IHC-P 1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.

Application notes

Is unsuitable for Flow Cyt or ICC/IF.

Function

Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response.
Tissue specificity

Strongly expressed in heart, lung, placenta and spleen.
Sequence similarities

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Cellular localization

Secreted.
Target information above from: UniProt accession O95998 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 10068 Human
- Entrez Gene: 84388 Rat
- Omim: 604113 Human
- SwissProt: O95998 Human
- Unigene: 591967 Human
Alternative names
- I18BP_HUMAN antibody
- IL-18BP antibody
- IL18 binding protein antibody
- IL18 BP antibody
- IL18 BPa antibody
- IL18BP antibody
- IL18BPa antibody
- Interleukin 18 binding protein antibody
- Interleukin-18-binding protein antibody
- MC51L 53L 54L homolog gene product antibody
- Tadekinig alfa antibody
- Tadekinig-alfa antibody
see all

Western blot - Anti-IL-18BP antibody [EP1088Y] (ab52914)

All lanes : Anti-IL-18BP antibody [EP1088Y] (ab52914) at 1/2000 dilution (purified)

Lane 1 : Rat kidney tissue lysate
Lane 2 : Rat heart tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 22 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 1% BSA/TBST.
Western blot - Anti-IL-18BP antibody [EP1088Y] (ab52914)

All lanes : Anti-IL-18BP antibody [EP1088Y] (ab52914) at 1/1000 dilution (purified)

Lane 1 : HeLa whole cell lysate
Lane 2 : K562 whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 22 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 1% BSA/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18BP antibody [EP1088Y] (ab52914)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling IL-18BP with purified ab52914 at 1/400. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-IL-18BP antibody [EP1088Y] (ab52914)

Anti-IL-18BP antibody [EP1088Y] (ab52914) at 1/500 dilution (unpurified) + HeLa cell lysate at 10 µg

Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 22 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18BP antibody [EP1088Y] (ab52914)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling IL-18BP with unpurified ab52914 at a dilution of 1/50.
Anti-IL-18BP antibody [EP1088Y] (ab52914)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab52914? Please let us know so that we can cite the reference in this datasheet.

ab52914 has been referenced in 9 publications.

Meng Q et al. IL-18BP Improves Early Graft Function and Survival in Lewis-Brown Norway Rat Orthotopic Liver Transplantation Model. Biomolecules 12:N/A (2022). PubMed: 36551229
Nakanishi Y et al. IL-18 binding protein can be a prognostic biomarker for idiopathic pulmonary fibrosis. PLoS One 16:e0252594 (2021). PubMed: 34086758
Zhou T et al. IL-18BP is a secreted immune checkpoint and barrier to IL-18 immunotherapy. Nature 583:609-614 (2020). PubMed: 32581358
Huang C et al. Chromatin Accessibility Regulates Gene Expression and Correlates With Tumor-Infiltrating Immune Cells in Gastric Adenocarcinoma. Front Oncol 10:609940 (2020). PubMed: 33469515
Sun H et al. MicroRNA-124 regulates cell pyroptosis during cerebral ischemia-reperfusion injury by regulating STAT3. Exp Ther Med 20:227 (2020). PubMed: 33193841
Gu C et al. Role of the thioredoxin interacting protein in diabetic nephropathy and the mechanism of regulating NOD-like receptor protein 3 inflammatory corpuscle. Int J Mol Med 43:2440-2450 (2019). PubMed: 31017263
Lin C et al. Melatonin attenuates traumatic brain injury-induced inflammation: a possible role for mitophagy. J Pineal Res 61:177-86 (2016). PubMed: 27117839
Lin L et al. Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma. Sci Rep 6:26757 (2016). IHC . PubMed: 27225603
Carbotti G et al. The IL-18 antagonist IL-18-binding protein is produced in the human ovarian cancer microenvironment. Clin Cancer Res 19:4611-20 (2013). IHC ; Human . PubMed: 23873689

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1-2 of 2 Abreviews or Q&A

Application

Western blot

Sample

Rat Cell lysate - whole cell (Kidney)

Gel Running Conditions

Reduced Denaturing (gel 12 %)

Loading amount

15 µg

Specification

Kidney

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 29 2014

Question

thank you for your answer, even if it was not satisfactory.
I know the basics of immunofluorescence and flow cytometry. What I need to know is the protocol that you optimized for using EP1088Y in flow cytometry, if there is one, so that I do not have to do all the optimization myself. If you state in the datasheet that EP1088Y is FC+, did you try it? Is the epitope resistant to PFA or to methanol or to both? Do you have a profile to show? No mention to flow cytometry is made in the references cited in the datasheet.
Thank you for your attention, sincerely

Abcam community

Verified customer

Asked on Apr 05 2013

Answer

Thank you for contacting us.

The recommended protocol can be found at the following link;

http://www.epitomics.com/products/support/fc

The antibody is guaranteed for Flow Cytometry and it would work. if it does not we will refund your money.

We always recommend our customers protocol optimization so these are necessary for best results.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam Scientific Support

Answered on Apr 05 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (9)

Customer reviews and Q&As

Western blot abreview for Anti-IL-18BP antibody [EP1088Y]