Recombinant Anti-IL-18BP antibody [EP1088Y] (ab52914)
![Western blot - Anti-IL-18BP antibody [EP1088Y] (ab52914)](https://www.abcam.com/ps/products/52/ab52914/Images/ab52914-253948-anti-il-18bp-antibody-ep1088y-western-blot.jpg "Western blot - Anti-IL-18BP antibody [EP1088Y] (ab52914)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1088Y] to IL-18BP
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-IL-18BP antibody [EP1088Y]
See all IL-18BP primary antibodies -
Description
Rabbit monoclonal [EP1088Y] to IL-18BP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide within Human IL-18BP aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: O95998 -
Positive control
- WB: HeLa and K562 cell lysates and rat kidney and heart tissue lysates. IHC-P: human tonsil tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1088Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 22 kDa.
|
| IHC-P |
1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 22 kDa. |
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IHC-P
1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response. -
Tissue specificity
Strongly expressed in heart, lung, placenta and spleen. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 10068 Human
- Entrez Gene: 84388 Rat
- Omim: 604113 Human
- SwissProt: O95998 Human
- Unigene: 591967 Human
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Alternative names
- I18BP_HUMAN antibody
- IL-18BP antibody
- IL18 binding protein antibody
see all
Images
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Western blot - Anti-IL-18BP antibody [EP1088Y] (ab52914)All lanes : Anti-IL-18BP antibody [EP1088Y] (ab52914) at 1/2000 dilution (purified)
Lane 1 : Rat kidney tissue lysate
Lane 2 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 22 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 1% BSA/TBST.
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![Western blot - Anti-IL-18BP antibody [EP1088Y] (ab52914)](https://www.abcam.com/ps/products/52/ab52914/Images/ab52914-253947-anti-il-18bp-antibody-ep1088y-western-blot.jpg)
Western blot - Anti-IL-18BP antibody [EP1088Y] (ab52914)All lanes : Anti-IL-18BP antibody [EP1088Y] (ab52914) at 1/1000 dilution (purified)
Lane 1 : HeLa whole cell lysate
Lane 2 : K562 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 22 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 1% BSA/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18BP antibody [EP1088Y] (ab52914)](https://www.abcam.com/ps/products/52/ab52914/Images/ab52914-253896-anti-il-18bp-antibody-ep1088y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18BP antibody [EP1088Y] (ab52914)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling IL-18BP with purified ab52914 at 1/400. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Western blot - Anti-IL-18BP antibody [EP1088Y] (ab52914)](https://www.abcam.com/ps/products/52/ab52914/Images/ab52914-65404-anti-il-18bp-antibody-ep1088y-western-blot.jpg)
Western blot - Anti-IL-18BP antibody [EP1088Y] (ab52914)Anti-IL-18BP antibody [EP1088Y] (ab52914) at 1/500 dilution (unpurified) + HeLa cell lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 22 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18BP antibody [EP1088Y] (ab52914)](https://www.abcam.com/ps/products/52/ab52914/Images/ab52914-65403-anti-il-18bp-antibody-ep1088y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18BP antibody [EP1088Y] (ab52914)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling IL-18BP with unpurified ab52914 at a dilution of 1/50.
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![Anti-IL-18BP antibody [EP1088Y] (ab52914)](https://www.abcam.com/ps/products/52/ab52914/Images/ab52914-6-benefits-of-recombinant-antibodies.png)
Anti-IL-18BP antibody [EP1088Y] (ab52914)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab52914 has been referenced in 9 publications.
- Meng Q et al. IL-18BP Improves Early Graft Function and Survival in Lewis-Brown Norway Rat Orthotopic Liver Transplantation Model. Biomolecules 12:N/A (2022). PubMed: 36551229
- Nakanishi Y et al. IL-18 binding protein can be a prognostic biomarker for idiopathic pulmonary fibrosis. PLoS One 16:e0252594 (2021). PubMed: 34086758
- Zhou T et al. IL-18BP is a secreted immune checkpoint and barrier to IL-18 immunotherapy. Nature 583:609-614 (2020). PubMed: 32581358
- Huang C et al. Chromatin Accessibility Regulates Gene Expression and Correlates With Tumor-Infiltrating Immune Cells in Gastric Adenocarcinoma. Front Oncol 10:609940 (2020). PubMed: 33469515
- Sun H et al. MicroRNA-124 regulates cell pyroptosis during cerebral ischemia-reperfusion injury by regulating STAT3. Exp Ther Med 20:227 (2020). PubMed: 33193841
- Gu C et al. Role of the thioredoxin interacting protein in diabetic nephropathy and the mechanism of regulating NOD-like receptor protein 3 inflammatory corpuscle. Int J Mol Med 43:2440-2450 (2019). PubMed: 31017263
- Lin C et al. Melatonin attenuates traumatic brain injury-induced inflammation: a possible role for mitophagy. J Pineal Res 61:177-86 (2016). PubMed: 27117839
- Lin L et al. Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma. Sci Rep 6:26757 (2016). IHC . PubMed: 27225603
- Carbotti G et al. The IL-18 antagonist IL-18-binding protein is produced in the human ovarian cancer microenvironment. Clin Cancer Res 19:4611-20 (2013). IHC ; Human . PubMed: 23873689