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  1. Link

    products/primary-antibodies/il-1ra-antibody-epr6483-ab124962.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-IL-1RA antibody [EPR6483] (ab124962)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (23)

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Western blot - Anti-IL-1RA antibody [EPR6483] (ab124962)
  • OI-RD Scanning - Anti-IL-1RA antibody [EPR6483] (ab124962)
  • Anti-IL-1RA antibody [EPR6483] (ab124962)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6483] to IL-1RA
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-IL-1RA antibody [EPR6483]
    See all IL-1RA primary antibodies
  • Description

    Rabbit monoclonal [EPR6483] to IL-1RA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IL-1RA aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P18510

  • Positive control

    • WB: A431 cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 8.40 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6483
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins

Associated products

  • Alternative Versions

    • Anti-IL-1RA antibody [EPR6483] - BSA and Azide free (ab226101)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab30132)
    • NIH/3T3 whole cell lysate (ab7179)
    • RAW 264.7 whole cell lysate (ab7187)
    • A-431 whole cell lysate (ab7909)
    • MOLT-4 whole cell lysate (ab7912)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/10000 - 1/50000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).

Observed band at >20 kDa is likely an unrelated protein. 

Notes
WB
1/10000 - 1/50000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).

Observed band at >20 kDa is likely an unrelated protein. 

Application notes
Is unsuitable for IHC-P.

Target

  • Function

    Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
  • Tissue specificity

    The intracellular form of IL1RN is predominantly expressed in epithelial cells.
  • Involvement in disease

    Microvascular complications of diabetes 4
    Interleukin 1 receptor antagonist deficiency
  • Cellular localization

    Cytoplasm and Secreted.
  • Target information above from: UniProt accession P18510 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3557 Human
    • Omim: 147679 Human
    • SwissProt: P18510 Human
    • Unigene: 81134 Human
    • Alternative names

      • DIRA antibody
      • F630041P17Rik antibody
      • ICIL 1RA antibody
      • ICIL-1RA antibody
      • ICIL1RA antibody
      • IL-1ra antibody
      • IL-1ra3 antibody
      • IL-1RN antibody
      • IL1 inhibitor antibody
      • IL1F3 antibody
      • IL1RA antibody
      • IL1RA_HUMAN antibody
      • IL1RN (IL1F3) antibody
      • IL1RN antibody
      • Interleukin 1 receptor antagonist antibody
      • Interleukin-1 receptor antagonist protein antibody
      • Intracellular IL 1 receptor antagonist type II antibody
      • Intracellular interleukin 1 receptor antagonist (icIL 1ra) antibody
      • IRAP antibody
      • MGC10430 antibody
      • MVCD4 antibody
      • Type II interleukin 1 receptor antagonist antibody
      see all

    Images

    • Western blot - Anti-IL-1RA antibody [EPR6483] (ab124962)
      Western blot - Anti-IL-1RA antibody [EPR6483] (ab124962)
      All lanes : Anti-IL-1RA antibody [EPR6483] (ab124962) at 1/10000 dilution

      Lane 1 : Wild-type A431 cell lysate
      Lane 2 : IL-1RA knockout A431 cell lysate

      Performed under reducing conditions.

      Predicted band size: 20 kDa
      Observed band size: 19 kDa why is the actual band size different from the predicted?



      False colour image of Western blot: Anti-IL-1RA antibody [EPR6483] staining at 1/10000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab124962 was shown to bind specifically to IL-1RA. A band was observed at 19 kDa in wild-type A431 cell lysates with no signal observed at this size in IL1RN knockout cell line ab273379 (knockout cell lysate ab275530). To generate this image, wild-type and IL1RN knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

    • OI-RD Scanning - Anti-IL-1RA antibody [EPR6483] (ab124962)
      OI-RD Scanning - Anti-IL-1RA antibody [EPR6483] (ab124962)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-IL-1RA antibody [EPR6483] (ab124962)
      Anti-IL-1RA antibody [EPR6483] (ab124962)

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (23)

    Publishing research using ab124962? Please let us know so that we can cite the reference in this datasheet.

    ab124962 has been referenced in 23 publications.

    • Zhang L  et al. Intravenous transplantation of olfactory ensheathing cells reduces neuroinflammation after spinal cord injury via interleukin-1 receptor antagonist. Theranostics 11:1147-1161 (2021). PubMed: 33391526
    • Gajawada P  et al. Myocardial Accumulations of Reg3A, Reg3? and Oncostatin M Are Associated with the Formation of Granulomata in Patients with Cardiac Sarcoidosis. Int J Mol Sci 22:N/A (2021). PubMed: 33923774
    • Chu LW  et al. Loganin prevents chronic constriction injury-provoked neuropathic pain by reducing TNF-a/IL-1ß-mediated NF-?B activation and Schwann cell demyelination. Phytomedicine 67:153166 (2020). PubMed: 31955133
    • Peng YJ  et al. Decoy Receptor 3 Promotes Preosteoclast Cell Death via Reactive Oxygen Species-Induced Fas Ligand Expression and the IL-1a/IL-1 Receptor Antagonist Pathway. Mediators Inflamm 2020:1237281 (2020). PubMed: 32587467
    • Liu J  et al. Inhibition of GSK3ß and RIP1K Attenuates Glial Scar Formation Induced by Ischemic Stroke via Reduction of Inflammatory Cytokine Production. Front Pharmacol 11:812 (2020). PubMed: 32595496
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-IL-1RA antibody [EPR6483]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver)
    Gel Running Conditions
    Non-reduced Denaturing (15%)
    Loading amount
    30 µg
    Specification
    liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 17 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-IL-1RA antibody [EPR6483]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (rat ligament)
    Permeabilization
    No
    Specification
    rat ligament
    Blocking step
    Biocare Rodent Block R as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 28 2014

    ELISA abreview for Anti-IL-1RA antibody [EPR6483]

    Poor
    Abreviews
    Abreviews
    Application
    ELISA
    Sample
    Human Recombinant protein (St. protein from Peprotech ELISA development kit ,)
    Specification
    St. protein from Peprotech ELISA development kit ,
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Type
    Sandwich (Capture)
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Toomas Neuman

    Verified customer

    Submitted Jan 29 2014

    ELISA abreview for Anti-IL-1RA antibody [EPR6483]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Recombinant protein (St. protein from Peprotech ELISA development kit ,)
    Specification
    St. protein from Peprotech ELISA development kit ,
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Toomas Neuman

    Verified customer

    Submitted Jan 29 2014

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