Recombinant Anti-IL-2 antibody [EPR16615-341] (ab243650)
![Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [EPR16615-341] (ab243650)](https://www.abcam.com/ps/products/243/ab243650/Images/ab243650-1-anti-il2-antibody-epr16615341-immunocytochemistry-el4il2.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [EPR16615-341] (ab243650)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16615-341] to IL-2
- Suitable for: Flow Cyt (Intra), ICC/IF
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-IL-2 antibody [EPR16615-341]
See all IL-2 primary antibodies -
Description
Rabbit monoclonal [EPR16615-341] to IL-2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: EL4.IL-2 cells. Flow Cyt (intra):EL4.IL-2 (mouse lymphoma T lymphocyte) treated with 80nM PMA + 1.34uM Ionomycin + 10.6uM Brefeldin A + 2uM Monensin for 6 h, Mouse splenocytes treated with 80nM PMA+1.34uM Ionomycin+10.6uM Brefeldin A+2uM Monensin.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16615-341 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/50.
|
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| ICC/IF | (1) |
1/50.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/50. |
|
ICC/IF
1/50. |
Target
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Function
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
Involvement in disease
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
Sequence similarities
Belongs to the IL-2 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 16183 Mouse
- SwissProt: P04351 Mouse
- Unigene: 14190 Mouse
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Alternative names
- Aldesleukin antibody
- IL 2 antibody
- IL-2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [EPR16615-341] (ab243650)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EL4.IL-2 cells labelling IL-2 with ab243650 at 1/50 (9.1 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing cytoplasmic staining in EL4.IL-2 cell line treated with Cell Stimulation Cocktail (80nM PMA + 1.34uM Ionomycin + 10.6uM Brefeldin A + 2uM Monensin) for 6 h. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-IL-2 antibody [EPR16615-341] (ab243650)](https://www.abcam.com/ps/products/243/ab243650/Images/ab243650-3-anti-il2-antibody-epr16615341-flow-cytometry-mouse-splenocytes-treated-with-cell-stimulation-cocktail.jpg)
Flow Cytometry (Intracellular) - Anti-IL-2 antibody [EPR16615-341] (ab243650)Intracellular flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse splenocytes treated with cell stimulation cocktail (80nM PMA+1.34uM Ionomycin+10.6uM Brefeldin A+2uM Monensin) for 6 hours (Right)/ Untreated control (Left). cells labelling IL-2 with ab243650 at 1/500 dilution (1ug)/ Left and Right (Red) compared with a isotype control. A Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with anti-CD3 conjugated to FITC. Then fixed with 2% PFA for 10min followed by intracellularly stained with ab243650. Gated on lymphocytes population. Data was kindly provided by an anonymous collaborator.
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![Flow Cytometry (Intracellular) - Anti-IL-2 antibody [EPR16615-341] (ab243650)](https://www.abcam.com/ps/products/243/ab243650/Images/ab243650-2-anti-il2-antibody-epr16615341-flow-cytometry-el4il2.jpg)
Flow Cytometry (Intracellular) - Anti-IL-2 antibody [EPR16615-341] (ab243650)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized EL4.IL-2 (mouse lymphoma T lymphocyte) treated with cell stimulation cocktail (80nM PMA + 1.34uM Ionomycin + 10.6uM Brefeldin A + 2uM Monensin) for 6 hours (Red)/ Untreated control (Green) cells labelling IL-2 with ab243650 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Anti-IL-2 antibody [EPR16615-341] (ab243650)](https://www.abcam.com/ps/products/243/ab243650/Images/ab243650-4-Benefits-of-Recombinant-antibodies.jpg)
Anti-IL-2 antibody [EPR16615-341] (ab243650)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab243650 has not yet been referenced specifically in any publications.