Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)](https://www.abcam.com/ps/products/245/ab245687/Images/ab245687-336943-anti-il-2-receptor-alpha-antibody-basiliximab-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)")
Key features and details
- Rabbit monoclonal [Basiliximab] to IL-2 Receptor alpha
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Rat, Human, Cynomolgus monkey, Rhesus monkey
- Isotype: IgG
Overview
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Product name
Anti-IL-2 Receptor alpha antibody [Basiliximab]
See all IL-2 Receptor alpha primary antibodies -
Description
Rabbit monoclonal [Basiliximab] to IL-2 Receptor alpha -
Host species
Rabbit -
Specificity
This antibody recognizes human IL-2R and binds to the epitope (116)ERIYHFV(122) within the extracellular domain of the CD25 subunit. Binding is abolished when 2 or more residues within the epitope are mutated. This epitope overlaps with the interaction site of CD25 and IL-2. CD25 is found in the high-affinity IL-2R (αβγc) – T cells only express CD25 upon activation. IL-2 binding to IL-2R on T cells modulates proliferation and differentiation.
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Tested applications
Suitable for: Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human, Cynomolgus monkey, Rhesus monkey -
Immunogen
Tissue, cells or virus corresponding to IL-2 Receptor alpha. CTC 16 cells.
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Positive control
- IHC-P: Rat spleen and thymus tissue. Flow Cyt: Human, rhesus monkey and cynomolgus monkey lymphocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Basiliximab -
Isotype
IgG -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use a concentration of 1 µg/ml.
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| IHC-P |
Use a concentration of 5 µg/ml.
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| Notes |
|---|
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Flow Cyt
Use a concentration of 1 µg/ml. |
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IHC-P
Use a concentration of 5 µg/ml. |
Target
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Function
Receptor for interleukin-2. -
Involvement in disease
Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
Sequence similarities
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3559 Human
- Entrez Gene: 25704 Rat
- Entrez Gene: 574300 Rhesus monkey
- Omim: 147730 Human
- SwissProt: P01589 Human
- SwissProt: P26897 Rat
- SwissProt: Q5MNY4 Rhesus monkey
- Unigene: 231367 Human
see all -
Alternative names
- Interleukin 2 receptor alpha chain antibody
- CD25 antibody
- CD25 antigen antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)
Formalin-fixed rat spleen tissue stained for IL-2 Receptor alpha with ab245687 at 5 µg/ml in immunohistochemical analysis. ab245687 also shows some cross-reactivity with rat in IHC.
This data was generated using the original Human IgG1 kappa version of this clone.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)](https://www.abcam.com/ps/products/245/ab245687/Images/ab245687-336944-anti-il-2-receptor-alpha-antibody-basiliximab-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)Formaldehyde-fixed rat thymus tissue stained for IL-2 Receptor alpha with ab245687 at 5 µg/ml in immunohistochemical analysis. Detected with an FITC-conjugated secondary antibody.
This data was generated using the original Human IgG1 kappa version of this clone.
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![Flow Cytometry - Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)](https://www.abcam.com/ps/products/245/ab245687/Images/ab245687-336945-anti-il-2-receptor-alpha-antibody-basiliximab-flow-cytometry.jpg)
Flow Cytometry - Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)Human lymphocytes were stained with an isotype control (Panel A) or ab245687 (Panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an AF488 conjugated donkey anti-rabbit antibody (ab150073).
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![Flow Cytometry - Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)](https://www.abcam.com/ps/products/245/ab245687/Images/ab245687-336946-anti-il-2-receptor-alpha-antibody-basiliximab-flow-cytometry.jpg)
Flow Cytometry - Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)Rhesus monkey lymphocytes were stained with an isotype control (Panel A) or ab245687 (Panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an AF488 conjugated donkey anti-rabbit antibody (ab150073).
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![Flow Cytometry - Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)](https://www.abcam.com/ps/products/245/ab245687/Images/ab245687-336947-anti-il-2-receptor-alpha-antibody-basiliximab-flow-cytometry.jpg)
Flow Cytometry - Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)Cynomolgus monkey lymphocytes were stained with an isotype control (Panel A) or ab245687 (Panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an AF488 conjugated donkey anti-rabbit antibody (ab150073).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab245687 has not yet been referenced specifically in any publications.